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Trpm2 (NM_138301) Mouse Untagged Clone
CAT＃: MC224620
Trpm2 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 2 (Trpm2), (10ug)
CNY 15,464.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9830168K16Rik; C79133; LTRPC2; Trp7; TRPC7; Trrp7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224620 representing NM_138301
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCTTGGACCGGAGAAGAACTGGCTCTGAGCAGGAGGAGGGCTTTGGGGTGCAGTCAAGGAGGG CCACTGACCTGGGCATGGTCCCCAATCTCCGACGAAGCAATAGCAGCCTTTGCAAGAGCAGGAGATTTCT GTGCTCTTTCAGCAGTGAGAAGCAAGAAAACCTTAGCTCATGGATTCCCGAGAATATCAAGAAGAAGGAG TGTGTGTACTTCGTGGAAAGTTCCAAACTCTCGGACGCAGGGAAGGTAGTGTGTGCGTGTGGTTATACCC ACGAGCAACACTTGGAGGTGGCCATCAAGCCACACACCTTCCAGGGCAAGGAGTGGGATCCAAAGAAACA CGTCCAAGAGATGCCCACAGATGCCTTTGGTGACATCGTTTTCACAGACCTGAGCCAGAAAGTGGGGAAG TATGTCCGGGTCTCCCAGGACACGCCCTCCAGTGTCATCTACCAGCTCATGACCCAGCACTGGGGCCTAG ATGTCCCCAACCTCCTCATCTCCGTGACCGGTGGGGCCAAGAACTTCAACATGAAGCTGAGGCTGAAGAG CATCTTCCGGAGAGGCTTGGTCAAGGTGGCTCAAACCACGGGGGCCTGGATCATCACTGGAGGATCCCAC ACAGGCGTGATGAAGCAGGTGGGCGAAGCGGTACGGGACTTCAGTCTGAGCAGCAGCTGCAAAGAAGGTG AAGTCATCACTATTGGCGTAGCCACGTGGGGCACCATCCACAACCGCGAGGGACTGATCCATCCCATGGG AGGCTTCCCCGCCGAGTACATGCTGGATGAGGAAGGCCAAGGGAACCTGACCTGCTTGGACAGCAACCAT TCCCACTTCATCTTGGTGGATGATGGGACCCACGGGCAATATGGTGTGGAGATTCCGCTGAGGACTAAGC TGGAAAAGTTCATCTCAGAGCAAACGAAGGAAAGGGGAGGTGTGGCCATCAAGATCCCCATTGTCTGCGT GGTGTTGGAGGGTGGCCCTGGCACTCTGCATACAATCTACAATGCCATCAACAATGGCACACCCTGCGTG ATAGTGGAGGGCTCTGGCCGAGTGGCTGACGTCATCGCTCAGGTAGCTACTCTGCCTGTCTCTGAGATCA CCATCTCCTTGATCCAGCAGAAGCTCAGCATATTCTTCCAGGAGATGTTTGAGACTTTCACCGAAAACCA GATTGTGGAATGGACCAAAAAGATCCAAGACATTGTCCGGAGGCGGCAGCTGCTGACGATCTTCCGGGAA GGCAAGGATGGTCAGCAGGATGTGGATGTGGCCATTCTGCAGGCGTTACTGAAAGCCTCTCGAAGCCAAG ACCACTTTGGCCACGAGAACTGGGACCACCAACTGAAGTTGGCTGTGGCCTGGAACCGCGTGGACATCGC TCGCAGTGAGATCTTCACCGATGAATGGCAGTGGAAGCCTGCAGATCTGCATCCCATGATGACGGCCGCT CTCATCTCCAACAAGCCTGAGTTCGTGAGGCTCTTTCTGGAGAATGGGGTGCGGCTCAAGGAGTTTGTCA CTTGGGATACTCTTCTCTGCCTGTACGAGAACCTGGAGCCGTCCTGTCTCTTCCACAGCAAGCTACAGAA GGTGCTGGCCGAAGAACAGCGCTTAGCCTATGCATCTGCAACACCCCGCCTGCACATGCACCATGTGGCC CAGGTGCTTCGTGAACTCCTGGGGGACTCCACGCAGCTGCTGTACCCCCGGCCCCGGTACACTGACAGGC CACGGCTCTCGATGACCGTGCCACACATCAAGCTGAACGTGCAGGGAGTGAGCCTCCGGTCCCTCTATAA GCGATCAACAGGCCACGTTACCTTCACCATTGACCCAGTCCGTGACCTTCTCATTTGGGCCGTTATCCAG AACCACAGGGAGCTGGCAGGCATCATCTGGGCTCAGAGTCAGGACTGTACTGCCGCAGCACTGGCCTGTA GCAAGATCCTGAAGGAGCTGTCCAAGGAGGAGGAAGATACAGACAGCTCTGAGGAGATGCTGGCACTGGC AGACGAGTTTGAGCACAGAGCTATAGGCGTCTTCACTGAGTGCTACAGGAAGGATGAGGAAAGAGCCCAG AAGCTGCTTGTCCGTGTGTCTGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTGGCCCTAGAGGCCAAGG ACATGAAATTCGTGTCTCATGGAGGCATCCAGGCTTTCCTAACCAAGGTGTGGTGGGGCCAGCTCTGTGT GGACAATGGCCTGTGGAGGATCATCCTGTGCATGCTGGCCTTCCCGCTGCTCTTCACCGGCTTCATCTCC TTCAGGGAAAAGAGGCTGCAGGCACTGTGTCGCCCAGCCCGCGTCCGCGCCTTCTTCAATGCGCCTGTGG TCATCTTCCACATGAATATCCTCTCCTACTTTGCCTTCCTCTGCCTGTTCGCCTACGTGCTCATGGTGGA CTTCCAGCCTTCTCCATCCTGGTGCGAGTACCTCATCTACCTGTGGCTCTTCTCCCTGGTGTGCGAAGAG ACTCGGCAGCTATTCTATGATCCTGATGGCTGTGGACTAATGAAGATGGCGTCCCTGTACTTCAGTGACT TCTGGAACAAACTGGACGTTGGGGCCATTCTGCTCTTCATAGTAGGACTGACCTGTCGGCTCATCCCAGC GACGCTGTACCCTGGGCGCATCATCCTGTCTTTGGACTTCATCATGTTCTGTCTCCGTCTCATGCACATC TTCACTATTAGCAAGACACTGGGGCCCAAGATAATCATCGTGAAGCGGATGATGAAGGACGTCTTCTTCT TCCTCTTTCTCCTGGCGGTGTGGGTGGTGTCCTTCGGCGTAGCTAAGCAGGCCATTCTCATACATAACGA GAGCCGCGTGGACTGGATCTTCCGTGGGGTTGTCTATCACTCTTACCTGACCATCTTTGGCCAGATCCCA ACCTACATTGACGGTGTGAATTTCAGCATGGACCAGTGCAGCCCCAATGGCACGGACCCCTACAAGCCTA AGTGTCCTGAGAGCGACTGGACGGGACAGGCACCTGCCTTCCCCGAGTGGCTGACTGTCACCCTGCTCTG CCTCTACCTGCTCTTTGCCAACATCCTGCTGCTTAACCTGCTCATCGCCATGTTCAACTACACCTTCCAG GAGGTGCAGGAACACACAGACCAGATCTGGAAATTCCAGCGCCACGACCTGATCGAGGAGTACCATGGCC GTCCCCCGGCACCTCCCCCACTCATCCTCCTCAGCCACCTGCAGCTCCTGATCAAGAGGATTGTCCTGAA GATCCCTGCCAAGAGGCATAAGCAGCTCAAGAACAAGCTGGAGAAGAACGAGGAGACAGCGCTCCTGTCT TGGGAACTGTACCTGAAGGAGAACTACCTGCAGAACCAGCAGTACCAGCAGAAACAGCGTCCAGAGCAGA AAATCCAAGACATCAGTGAGAAAGTGGACACCATGGTGGATCTGCTGGACATGGACCAGGTGAAGAGGTC AGGCTCCACAGAGCAGAGACTGGCTTCCCTGGAGGAACAGGTGACTCAGGTGACCAGAGCTTTGCACTGG ATCGTGACAACCCTGAAGGACAGTGGCTTTGGCTCAGGAGCAGGTGCGCTGACCCTGGCACCCCAGAGGG CCTTCGATGAGCCAGATGCTGAGCTGAGTATCAGGAGGAAAGTAGAGGAACCAGGAGATGGTTACCACGT GAGCGCCCGGCATCTCCTCTATCCCAATGCCCGCATCATGCGCTTCCCCGTGCCTAACGAGAAGGTGCCT TGGGCGGCAGAGTTTCTGATCTACGATCCTCCCTTTTACACCGCTGAGAAGGATGTGGCTCTCACAGACC CCGTGGGAGACACTGCAGAACCTCTGTCTAAGATCAGTTACAACGTCGTGGATGGACCGACGGACCGTCG CAGCTTCCATGGAGTCTACGTGGTCGAGTATGGGTTCCCGTTGAACCCCATGGGCCGCACAGGGTTGCGT GGTCGTGGGAGCCTCAGCTGGTTTGGTCCCAACCACACTCTGCAGCCAGTTGTCACCCGGTGGAAGAGGA ACCAGGGTGGAGCCATCTGCCGGAAGAGTGTCAGGAAGATGCTGGAGGTGCTAGTGATGAAGCTGCCTCG CTCTGAGCACTGGGCCTTGCCTGGGGGCTCTAGGGAGCCAGGGGAGATGCTACCACGGAAGCTGAAACGG GTCCTCCGGCAGGAGTTCTGGGTGGCCTTTGAGACCTTGCTGATGCAAGGTACAGAGGTATACAAAGGGT ACGTGGATGACCCAAGGAACACAGACAATGCCTGGATCGAGACAGTGGCTGTCAGCATCCATTTTCAGGA CCAGAATGATATGGAGCTGAAGAGGCTGGAAGAGAACCTGCACACTCATGATCCAAAGGAGTTGACCCGT GACCTGAAGCTGTCTACTGAATGGCAGGTGGTAGACCGGCGGATCCCTCTGTATGCGAACCACAAGACCA TCCTCCAGAAGGTGGCCTCACTGTTTGGAGCTCACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_138301 |
| Insert Size | 4521 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC141391, AAI41392 |
| RefSeq Size | 7292 bp |
| RefSeq ORF | 4521 bp |
| Locus ID | 28240 |
| UniProt ID | Q91YD4 |
| Gene Summary | Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:11804595, PubMed:19454650, PubMed:21753080, PubMed:22493272). Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity (By similarity). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:21753080). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (PubMed:21753080). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (By similarity). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:27533035, PubMed:27562954). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:19454650, PubMed:21753080, PubMed:22493272). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius) (PubMed:22493272, PubMed:25817999). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis (PubMed:27533035, PubMed:27562954). Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (PubMed:20921208, PubMed:25817999). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection (PubMed:21709234). Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro) (PubMed:22493272). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (PubMed:21753080). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca(2+) and Zn(2+) levels (By similarity). Confers susceptibility to cell death following oxidative stress (PubMed:25562606).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225380 | Trpm2 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 2 (Trpm2), (10ug) |
CNY 16,530.00 |
|
| MR225380 | Trpm2 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 2 (Trpm2) |
CNY 15,464.00 |
|
| MR225380L3 | Lenti ORF clone of Trpm2 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 2 (Trpm2) |
CNY 16,910.00 |
|
| MR225380L4 | Lenti ORF clone of Trpm2 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 2 (Trpm2) |
CNY 16,910.00 |
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