Prrc2b (NM_172661) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5830434P21Rik; AI173903; Bat2l; Bat2l1; D430039P21; mKIAA0515
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224541 representing NM_172661 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGATCGTTTGGGGCAAATAACCCAGGGCAAGGATGGGAAAAGCAAGTACTCGACTCTCAGCCTGT TTGACAAGTATAAAGGGAGGTCAGTAGGCGCTGTCAGGTCCTCAGTTATTCCTAGACATGGCTTACAGAG TCTCGGGAAAGTTGCCACAGCCCGGCGTATGCCACCGCCTGCAAACCTGCCAAGCCTGAAGTCTGAAAAC AAAGGAAACGACCCCAACATCGTGATAGTTCCCAAGGACGGGACAGGATGGGCCAACAAGCAGGACCAGC AAGACCCAAAGAGTTCCAGTGTGACGGCCTCTCAGCCGCCGGAGTCGCAGCCGCAGCCGGGTTTGCAGAA ATCTGTCTCCAATTTGCAGAAACCGACACAGTCTATCAGTCAGGAGAACACAAATTCAGTGCCAGGTGGA CCAAAGTCATGGGCACAGCTGAGTGGAAAGCCAGTCGGACACGAAGGTGGTTTAAGGGGCTCAAGCCGAC TGTTATCCTTCTCTCCCGAGGAATTTCCGACGCTGAAAGCAGCTGGAGGGCAGGACAAGGCTGGCAAAGA AAAGGGCGCCTTAGATCTGTCGTATGGGCCAGGACCAAGCCTCCGCCCTCAGAATGTGACAAGCTGGAGG GAGGGCGGTGGGCGGAACATCATTTCTGCCGCCTCTCTGAGCGCCTCCCCAACAGAGCTGGGCAGCAGGA ACGCCAGTGGGGCAGACGGAGCCCCTTCCTTGGCATGCACCAGCGATTCTAAGGAGCCCTCTCTCCGCCC AGCTCAGCCCAGCCGCAGAGGGGCCTCCCAATTCATGGGGCACGGTTACCAGCCACCCACGTACCATGAC ATGCTCCCAGCGTTTATGTGTTCACCACAGTCATCAGAGAACCAGACTACGGTGGAACGAAGCTCTTTTC CCCTTCCTCAGCTCCGCCTGGAACCCCGTGTTCCTTTCAGACAGTTCCAGATGAACGACCAAGATGGGAA AGAAAGGCCAGGCGTGGCACGGCCAGTGCGCCCACTGAGGCAGCTGGTGGAGAGGGCACCAAGGCCCACC ATCATCAACGCGGAAAACCTGAAGGGCTTGGATGATCTGGACACTGATGCGGATGATGGGTGGGCAGGCC TCCACGAGGAAGTGGACTATTCCGAGAAACTGAAGTTCAGTGATGATGAAGATGAGGAGGATGTTGTGAA GGATGGCCGGTCAAAGTGGAACAACTGGGATCCAAGGCGGCAGCGGGCGCTGTCATTGAGCTCTGCAGAC AGCACTGACGCCAAGCGCACTCAGGAGGAAGGGAAGGACTGGAGTGGAACAGCAGGTGGATCCCGTGTCA TCCGAAAGGTGCCAGAGCCTCAGCCTCCTTCCAGGAAGCTTCACAGTTGGGCCTCTGGGCCTGACTACCA GAAACCCACAATGGGCAGCATGTTCCGCCAGCACTCCGCTGAGGACAAGGAGGACAAGCCACCCCCACGG CAGAAGTTCATCCAGTCAGAGATGTCCGAGGCCGTGGAACGAGCCCGAAAGCGCAGGGAAGAAGAAGAGC GCCGGGCCCGAGAGGAGAGGCTGGCTGCCTGTGCTGCCAAACTCAAGCAGCTAGACCAGAAGTGCAGGCA GGCTCAGAAGGCCAATGAGACCCCGAAGCCAGTGGAAAAGGAAGTCCCCCGATCCCCGGGCATTGAGAAG GTCTCCCCGCCAGAGAATGGGCCTGTTGTCCGCAAAGGCTCCCCAGAGTTCCCTGTCCAAGAAGCACCCA CCATGTTCTTGGAAGAGACACCTGCAACATCCCCAACAGTCGCTCAGAGCAATAGCAGCAGCAGCAGTAG CAGCAGTAGCAGCATCGAGGAGGAAGTCCGGGAGTCCGGGTCCCCTGCACAGGAGTTCAGCAAGTACCAG AAGTCCCTTCCTCCCCGTTTCCAGCGCCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAGCAGCAGCAGC AGGAGCAGCTGTACAAGATGCAGCACTGGCAGCCGGTCTACCCCCCACCTTCTCACCCCCAGCGCACCTT CTACCCACACCACCCCCAGATGCTGGGCTTTGACCCACGGTGGATGATGATGCCTTCCTACATGGACCCA CGCATCACCCCGACACGGACGCCCGTGGACTTCTACCCCTCAGCCCTGCATCCTTCAGGACTGATGAAGC CCATGATGCCCCAAGAGTCCCTCAGTGGGACTGGCTGTCGCTCTGAAGATCAAAACTGTGTGCCCTCGCT CCAAGAACGGAAAGTGACCGCCCTCGACCCAGCTCCTGTGTGGAGCCCAGAGGGCTACATGGCACTGCAG AACAAAGGCTACTCACTGCCCCACCCGAAGTCTGCTGACACTCTGGCCATGGGCATGCATGTCAGGTCTC CAGATGAGGCCTTGCCTGGAGGCCTTGGCAGCCATTCACCCTATGCCTTGGAGAGAACCACTCACGCCAG CTCTGACGGTCCTGAAACCCCCAGTAAGAAATCAGAGAGAGAGGTTTCTTTGCCTACCCAGAGGGCTAGT GAGCAGGAAGAGGCCCGGAAGCAGTTTGACCTGGGCTATGGAAATGCTCTCATTGACAACTGCGCCTCCA GCCCTGGGGAAGAGAATGAAGCAAGCTCTGTGGTGGGTGAAGGCTTCATTGAAGTCCTGACCAAGAAGCA GCGTCGCCTGCTGGAGGAGGAGAGAAGAAAAAAAGAACAGGCTGCTCAGGTGCCTGTCAAAGGTCGGGGC CTTTCATCCCGGATTCCTCCTCGGTTTGCTAAAAAGCAGAACGGCCTGTGTCTGGAGCAAGATGTGACTG TGCCTGGCAGCAGCCTGGGCACTGAGATCTGGGAGAACAGCAGCCAAGCCCTCCCTGTGCAGGGCGCCGC CAGTGACTCATGGAGGACAGCTGTCACTGCCTTCAGCAGTACTGAGCCTGGCACCTCAGAGCAGGGGTTT AAGAGCAGCCAGGGAGATAGTGGCGTTGACTTGAGTGCTGAGTCTCGGGAGTCGTCTGCGACCTCCTCAC AGCGCAGTTCCCCATATGGCACTCTCAAGCCTGAGGAAATTAGCGGGCCTGGCCTGGCGGAATCTAAGGC CGACAGCCACAAGGACCAAGCTCAGAAGCAGGCGGAGCACAAGGACTCAGAACAAGGCTCTGCACAGAGC AAGGAGCACAGACCAGGACCCATCGGCAACGAGCGGTCTCTGAAAAACAGAAAGGGCTCTGAGGGGGCCG AGCGGCTGCCAGGGGCCGTTGTCCCGCCTGTTAATGGGGTGGAGATTCACGTGGACTCCGTGCTGCCGGT GCCGCCCATTGAGTTCGGAGTCAGTCCAAAAGACTCTGATTTCAGCCTGCCACCCGGGTCTGTTTCTGGT CCTGTAGGGAATCCAGTGGCCAAGCTTCAGGACGTCTTGGCTAGTAATGCAGGACTGACGCAGAGTATTC CCATCCTGCGGCGGGATCACCACATGCAGAGAGCCATCGGCCTCTCTCCCATGTCTTTCCCCACCGCAGA CCTCACACTAAAGATGGAGTCTGCACGCAAGGCTTGGGAAAACTCCCCCAGTTTGCCAGAGCAGAGCTCT CCAGGAGGTGCAGGCTCGGGCATCCAGCCTCCATCCTCTGTGGGCGCCTCCAATGGGGTCAACTACAGCT CCTTTGGTGGAGTGTCGATGCCGCCCATGCCCGTGGCTTCTGTAGCGCCTTCTGCTTCGATACCAGGCAG CCACCTCCCACCTCTGTACCTGGATGGCCACGTGTTTGCAAGTCAGCCCCGACTGGTTCCTCAAACGATA CCTCAGCAGCAGAGCTACCAGCAGGCCGCCACTGCCCAGCAGATCCCGATATCCCTTCACACGTCTCTGC AGGCCCAGGCCCAGCTTGGGCTGAGAGGTGGGCTCCCCGTCTCCCAGTCCCAGGAGATCTTCAGCTCCCT GCAGCCCTTCAGGTCTCAGGTGTACATGCACCCCAGCCTGTCACCACCCAGCACGATGATCCTCTCAGGG GGTACAGCCTTGAAGCCCCCATACTCTGCATTTCCTGGCATACAGCCCTTGGAGATGGTGAAGCCCCAGT CTGGCTCACCCTACCAGCCCATGAGTGGAAACCAGGCCCTGGTCTACGAGGGCCAGCTTGGCCAGGCTGC CGGGCTGGGCACCTCCCAGATGCTGGATTCCCAGCTCCCACAGCTCACTATGCCACTGCCTCGGTATGGC TCCGGGCAGCAGCCACTGATCCTGCCACAGTCCATCCAGCTGCCTCCAGGACAGAGTCTCTCTGTTGGAG CCCCCCGGAGGGTTCCTCCACCTGGCTCCCAACCTCCCGTCCTGAACACCAGCAGAGAGTCCGCTCCGAT GGAGTTGAAAGGCTTCCACTTTGCCGACAGTAAACAGAATGTTCCTACTGGAGGGTCCGCACCATCCCCG CAGGCATACAGGCAAAGCGAGTGGATGAAAAACCCGGCCTGGGAACCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172661
Insert Size	4461 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_172661.3, NP_766249.2
RefSeq Size	8564 bp
RefSeq ORF	4461 bp
Locus ID	227723
UniProt ID	Q7TPM1

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