Nfat5 (NM_133957) Mouse Untagged Clone
CAT#: MC224498
Nfat5 (untagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a, (10ug)
CNY 18,720.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI225870; B130038B15Rik; CAG-8; CAG80; mKIAA0827; NFATL1; nfatz; OREBP; TonEBP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224498 representing NM_133957
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACA GCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCA GTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCACCA CCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGC AGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGA TAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGAT TGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGC TTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGAGT AAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGGTA CAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCTGTAAAAGACAGAACAC AGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTTGCAGGTATTTGTGGGCAA TGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACTGGGCGAAATACAACTCCC TGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATCCTAGCAACAACATGACAC TGGCGGTGGACTGTGTTGGAATATTGAAGCTGAGGAATGCCGATGTTGAAGCCAGAATTGGAATTGCTGG ATCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACAAGGAAAGATGGCTCTACT CTGACATTGCAGACACCTTCTTCCCCCATTTTATGCACTCAGCCAGCAGGAGTTCCTGAGATCTTAAAGA AAAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTATTTTTAATTGGGAAAAACTTTCTGAAAGG AACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCAGAAGCTGAAATTGACATG GAGTTATTCCATCAGAACCATCTTATTGTGAAGGTTCCTCCGTATCATGACCAACATATAACTTTGCCTG TATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCAGCCATTTACGTACACTCC GGATCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAAAAGGAGATATCTAGTCCAGCAAGACCTTGCTCT TTTGAAGAAGCCATGAAAGCAATGAAAACGACTGGATGTAACGTAGATAAGGTGACTATCCTTCCTAATG CCCTGATCACTCCACTCATATCAAGCAGTATGATTAAGACTGAAGATGTTACTCCAATGGAAGTAACTTC AGAAAAAAGATCTTCCCCAATTTTTCAGACTACAAAGAGCATTGGATCAACCCAGCAAACCTTAGAAACT ATTTCTAACATAGCGGGGGGTGCACCCTTCTCCTCACCGTCGTCATCTTCCCACTTAACTCCTGAAAGTG AGAATCAGCAGCAGCTTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATCCAGACACAGGACAT CTCACAGCCCGGGACCTTTCCCGCAGTTTCTGCTGCTAGTCAGCTGCCCAGCAGCGATGCACTACTGCAG CAGGCAACACAGTTTCAGACAAGAGAAGCTCAGTCCAGAGACACAATACAGTCAGATACAGTGGTTAACT TGTCACAGTTAACTGAAGCATCACAGCAGCAGCAATCCCCACTACAAGAACAAGCACAAACGTTACAGCA GCAGATACCATCAAATATTTTTCCATCGCCAAGCAGTGTCAGCCAGCTCCAGAGTACTATTCAGCAGCTG CAAGCAGGAAGTTTCACAGGCAGCGCTGCTGGCGGCAGGAGTGGAAGCGTTGATTTGGTCCAGCAGGTTT TAGAGGCACAACAGCAGTTATCGTCTGTTCTGTTTTCTACTCCAGATGGGAATGAGAATGTTCAAGAACA GCTTAATGCAGACATTTTCCAAGTCAGTCAAATTCAAAATAGTGTAAGCCCTGGAATGTTTTCTTCAGCG GAGTCTGCAGTTCACACTAGACCAGATAACTTACTACCTGGGAGGGCTGACAGCGTCCATCAACAGACTG AAAATACACTGTCTAATCAGCAGCAGCAGCAACAGCAGCAGCAGCAAGTGATGGAGTCATCAGCTGCAAT GGTGATGGAGATGCAGCAGAGCATTTGCCAAGCAGCTGCCCAGATCCAGTCAGAGCTGTTTCCTTCAGCT GCTTCAGCAAGTGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTCACATGATGAGTGCACTGC CTACCAACGAGGACATGCAGATGCAGTGTGAGCTGTTCTCGTCTCCCCCTGCAGCTTCTGGAAACGAAAC TTCCACAACCACCACGCCACAGGTGGCAACCCCTGGCTCCACCATGTTTCAGACACCAAGTTCAGGAGAT GGAGAAGAAACTGGAGCACAAGCAAAACAGATTCAGAACAGCGTCTTTCAGACAATGGTCCAAATGCAGC GCAGTGGTGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAATATAATGAGTGTTCAGAATAA TGGTACCCAGCAACAAGGGAATAGCTTGTTCCAGCAAGGGAGCGAGATGATGTCACTTCAGTCTGGGAAC TTTTTGCAGCAGTCCTCTCATTCACAGGCTCAGCTCTTTCATCCTCAAAATCCTATTGCTGATGCTCAGA ACCTTTCCCAGGAAACTCAAGGTTCAATTTTTCATAGTCCAAATCCTATTGTCCACAGTCAAACTTCCAC AGCATCCTCTGAACAACTGCAGCCTTCAATGTTTCACTCTCAGAATACCATTGCTGTGTTACAGGGCTCT TCAGTTCCTCAAGACCAGCAGTCACCCAACATATTTCTTTCCCAAAGTTCTATCAATAATCTTCAAACTA ACACAGTAGCCCAAGAAGAGCAGATTTCATTTTTTGCAGCACAGAACTCAATTTCTCCACTTCAGTCAAC ATCAAACACTGAGCAGCAAGCTGCTTTCCAACAGCAGCCTCCAATCTCACACATCCAGACCCCTATTCTT TCCCAGGAACAGGCACAACCCTCTCAGCAAGGGTTATTTCAGCCTCAGGTGGCCCTTGGCTCCCTGCCTC CTAATCCAATGCCTCAAAACCAGCAAGGTCCAATTTTCCAAACACAGCGCCCAATAGTTGGCATGCAGAG TAACTCTCCATCCCAGGAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAGCAGCAACAGCAACAACAA CAGCAACAGAGCATTTTATTCAGTAATCAGAATGCCATGGCTACAATGGCCTCCCAGAAGCAGCCACCAC CAAACATGATGTTTAGCCCAAACCAGAACCCAATGGCTAGTCAGGAGCAGCAGAACCAGTCAATTTTTCA CCAGCAAAGTAATATGGCCCCAATGAACCAAGAGCAGCAGCCCATGCAATTTCAGAATCAGCCTACGGTT TCCTCACTTCAGAACCCAGGTCCTACGCAGTCTGAGTCACCACAGACCTCCTTGTTCCATAGCTCTCCTC AGATCCAGTTGGTTCAAGGGTCACCTAGTTCTCAAGATCAGCAAGTAACACTCTTCCTCTCTCCAGCATC CATGTCTGCATTGCAAACCAGTATAAACCAACCAGACATGCAGCAGTCTCCTCTTTATTCCCCGCAGAAC AACATCCCTGGAATCCAAGGAAGCACTTCTTCACCCCAGCCACAGGCTACTTTATTTCACAACACTACAG GAGGCACAATAAACCAAATACAAAATTCTCCTGGCTCATCTCAGCAGACTTCAGGAATGTTCTTATTTGG CATTCAAAATAATTGTAGTCAGCTTTTAACCTCCGGACCAGCTACATTGCCAGATCAGCTGATGGCCATA AATCAGCAGGGCCAACCACAAAATGAGGGCCAATCTTCTGTGACAACACTTCTTTCTCAGCAAATGCCAG AGACTTCCCCACTGGCCTCCTCTGTGAACAGCAGTCAGAACATGGAAAAGATTGATTTGCTTGTTTCATT GCAAAGCCAAGGGAACAATTTAACCGGCTCCTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_133957 |
Insert Size | 4377 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_133957.3, NP_598718.2 |
RefSeq Size | 13451 bp |
RefSeq ORF | 4377 bp |
Locus ID | 54446 |
UniProt ID | Q9WV30 |
Gene Summary | Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (a) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon. This results in a protein (isoform a) with a shorter N-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG223442 | Nfat5 (tGFP-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5) transcript variant a, (10ug) |
CNY 15,960.00 |
|
MR223442 | Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a |
CNY 14,540.00 |
|
MR223442L3 | Lenti ORF clone of Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a |
CNY 16,440.00 |
|
MR223442L4 | Lenti ORF clone of Nfat5 (mGFP-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a |
CNY 16,440.00 |