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Ythdc2 (NM_001163013) Mouse Untagged Clone
CAT＃: MC224492
Ythdc2 (untagged) - Mouse YTH domain containing 2 (Ythdc2), (10ug)
CNY 18,620.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 3010002F02Rik; BC037178 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224492 representing NM_001163013
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCCGAGCAGCGTCTCGCCGCGTCCGCCGGCTCCTAGCGGAGGCGGCACGGGCGGCGGTGGAG GTGGCAGTGGCGGCGGCGGTGGCGGTGGCGGCGGCGGCCCAGCTTCGTGTGGTCCTGGAGGCGGTGGCCG GGCCAAGGGCCTCAAAGACATTCGTATCGACGAGGAGGTGAAGATCGCCGTCAACATCGCTCTGGAGCGC TTCCGCTACGGTGACCAGAGAGAAATGGAATTCCCTTCTTCTTTGACCAGTACGGAAAGAGCCTTTATTC ACCGACTAAGTCAATCTCTTGGTTTAGTTTCTAAAAGTAAAGGAAAGGGAGCAAATAGATACCTAACTGT GAAGAAGAAAGATGGATCAGAAACAGCTCATGCAATGATGACCTGTAATTTGACTCATAACACAAAACAT GCTGTTAGGAGCCTAATTCAGAGATTTCCTGTCACCAATAAAGAACGTACTGAACTCCTACCTAAAACAG AAAGAGGAAATGTGTTTGCAGTTGAAGCTGAAAACCGAGAAATGAGCAAGACAAGCGGGCGACTCAACAA TGGCATTCCTCAAGTTCCTGTGAAAAGGGGAGAGTCTGAATTTGACTCATTCAGGCAGTCTTTACCAGTT TTTGAGAAACAAGAAGAAATTGTTAAAATAATTAAGGAAAATAAAGTAGTTTTGATAGTAGGAGAAACTG GGTCTGGAAAGACCACACAGATTCCTCAGTTCCTGTTAGATGATTGCTTTAAAAATGGCATCCCCTGCCG AATATTTTGTACTCAACCAAGACGACTAGCAGCGATTGCTGTGGCTGAAAGAGTTGCTGCAGAGAGAAGA GAAAGAATTGGTCAAACAATTGGTTATCAGATCCGATTAGAAAGCAGGGTTTCGCCAAAGACACTTCTGA CATTTTGCACTAATGGGGTATTGCTTCGTACATTGATGGCAGGTGATAGCACATTGTCAACTGTGACCCA TGTTATTGTGGATGAAGTGCATGAAAGGGATCGATTCAGTGATTTTTTGCTTACTAAGTTAAGAGATTTG TTGCAAAAGCACCCAACTTTGAAACTAATTCTTTCGAGTGCTGCCTTGGATGTGAACCTCTTCATAAGAT ATTTTGGAAGTTGTCCAGTGATATATATACAGGGAAGACCATTTGAAGTAAAAGAAATGTTTCTGGAAGA TATTTTAAGAACTACAGGATATACCAACAAAGAAATGTTAAAGTACAAAAAGGAAAAGCAACGAGAGGAG AAACAACAGACCACCCTCACAGAGTGGTACTCAGCTCAAGAGAATACCTTCAAGCCTGAGTCTCAGAGGC AGAGAGCTGTTGCTAGTGTGTCTGAAGAGTATGACTTATTGGATGATGGAGGTGATGCTGTCTTCAGTCA GCTGACAGAAAAAGATGTGAATTGCCTTGAACCATGGTTAATCAAGGAGATGGATGCCTGCCTTTCTGAC ATATGGCTGCATAAAGATGTTGATGCTTTTGCTCAGGTCTTTCATCTTATTTTAACTGAAAATGTTAGTG TTGATTATAGGCATAGTGAGACCAGTGCAACAGCTCTGATGGTTGCTGCAGGACGGGGCTTTACGAGCCA AGTAGAGCAGTTAATTAGTATGGGAGCCAATGTCCACAGTAAAGCATCAAATGGCTGGATGGCTTTAGAT TGGGCTAAGCACTTTGGACAGACTGAAATTGTGGATCTTCTAGAATCTTACAGTGCTTCATTGGAATTTG GAAATCTAGATGAAAGTTCCTTGGTTCAGACAAATGGGAATGACCTCAGTGCAGAAGACAGAGAGCTGTT GAAAGCTTACCATCATAGTTTTGATGATGAGAAAGTAGACTTGGATTTGATCATGCATCTCCTATATAAT ATCTGCCATAGCTGTGATGCTGGTGCAATATTAATTTTTCTACCTGGATATGATGAAATTGTTGGTTTGA GGGATCGTATCCTCTTTGATGACAAGCGGTTTGCTGACAATACACATAGGTATCAAGTCTTTATGCTTCA TTCAAATATGCAGACATCTGATCAAAAGAAGGTATTAAAAAATCCACCTGCAGGTGTCCGAAAAATAATC CTTTCCACCAATATTGCTGAGACGAGCATCACTGTCAATGATGTTGTGTTTGTTATTGATTCTGGTAAGG TGAAAGAGAAATCCTTTGATGCACTGAATTTTGTTACAATGTTAAAAATGGTATGGATTTCCAAAGCTAG TGCAATACAGCGCAAAGGCAGAGCGGGACGGTGTAGACCTGGAATTTGTTTCCGTCTGTTTAGTAGACTT CGATTTCAGAACATGCTGGAATTTCAGACGCCAGAACTCTTGAGAATGCCATTGCAGGAACTTTGTTTAC ATACCAAGCTGTTAGCCCCAGTTAACTGCACCATTGCCGACTTCCTTATGAAAGCCCCTGAACCGCCTCC AGCTTTAATTGTGAGAAATGCTGTGCAGATGCTTAAGACAATAGATGCAATGGATGCATGGGAAGATCTG ACTGAACTTGGGTATCATTTGGCTGATTTGCCAGTAGAACCGCATCTTGGGAAAATGGTCCTGTGTGCAG TGGTTTTGAAGTGTCTGGACCCCATCCTTACAATTGCCTGCACGCTCGCTTACCGTGACCCTTTTGTCCT GCCTACCCAGGCCTCTCAGAAACGGGCAGCTATGCTTTGTAGGAAACGCTTCACTGCAGGGACTTTCAGT GACCACATGGCACTTCTGAGAGCATTTCAGGCATGGCAGAAAGCACGAAGTGATGGGTGGGAGCGAGCCT TTTGTGAAAAGAATTTTCTTTCACAAGCTACTATGGAAATCATTATAGGCATGAGAACACAGTTGCTTGG TCAGCTTAGAGCATCAGGTTTTGTTAGAGCACGAGGTGGTGGTGACATTCGAGATGTTAATACAAACTCC GAGAACTGGGCTGTTGTTAAAGCTGCATTGGTAGCAGGCATGTATCCTAATTTAGTCCACGTGGACAGAG AGAATGTAATATTGACAGGGCCAAAGGAAAAGAAAGTACGCTTTCATCCCACTTCAGTTCTCAGTCAACC TCAATACAAAAAGATTCCTCCCGCCAATGGTCAGGCTGCAGCGATTCAGGCATTGCCCACAGATTGGCTT ATTTATGATGAAATGACCAGAGCCCATCGAATTGCTAATATCAGATGTTGTTCAGCAGTGACACCTGTTA CTGTCCTGGTGTTCTGTGGACCAGCAAGACTGGCAAGTAACGCTCTTCAGGAACCTTCATCCTTTAGAGC GGATGGTATTCCCAATGACAGCAGTGACAGTGAGATGGAAGACAGAACTACTGCCAATTTGGCTGCTTTG AAGCTTGATGAATGGCTTAACTTCAAACTAGAGCCAGAGGCTGCCAGTTTATTGCTACAGCTCAGACAGA AGTGGCATAGTTTGTTTTTACGCCGAATGAGAGCGCCATCTAAACCTTGGTCTCAAGTCGATGAAGCTAC CATACGAGCAATTATAGCTGTTTTAAGCACTGAAGAACAGTCTGCAGGTTTACAGCAGCCATCTGGAATT GGCCAGCGGCCAAGACCCATGTCTTCAGAAGAACTTCCTTTGGCTTCATCTTGGAGATCCAATAATAGTA GGAAAAGTACAGCTGACACTGAGTTTGCGGATGGATCTACTACTGGAGAAAGAGTACTGATGAAATCTCC ATCTCCAGCCTTACATCCACCTCAGAAGTACAAAGATAGAGGAATTTTACATCCTAAGCGGAGCACCGAT GACCGGTCCGATCAATCATCTGTGAAATCCACAGACAGCAGCAGTTACCCAAGTCCTTGTGCCAGCCCCT CTCCTCCATCCTCAGGGAAGGGATCAAAATCTCCTTCACCAAGACCAAACATGCCTATTCGTTACTTCAT AATGAAGAGTAGCAATCTGAGAAACCTTGAAATTTCTCAACAGAAGGGTATCTGGTCTACAACCCCTAGT AATGAGCGGAAGCTAAATCGAGCGTTTTGGGAGAGCAGCATGGTTTACTTGGTATTTTCTGTTCAAGGAT CTGGACATTTCCAGGGATTTTCTAGGATGTCTTCTGAGATAGGAAGGGAGAAGAGCCAGGACTGGGGGTC AGCTGGACTAGGTGGAGTATTTAAAGTGGAGTGGATACGCAAAGAAAGCCTTCCCTTTCAGTTTGCACAC CATTTACTCAATCCTTGGAACGACAACAAGAAAGTACAGATAAGCAGAGATGGGCAGGAGCTAGAACCTC AGGTTGGGGAACAGTTGCTCCAGTTATGGGAACGCCTTCCATTGGGAGAAAAAACAACTTCTGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001163013 |
| Insert Size | 4338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001163013.1, NP_001156485.1 |
| RefSeq Size | 6299 bp |
| RefSeq ORF | 4338 bp |
| Locus ID | 240255 |
| UniProt ID | B2RR83 |
| Gene Summary | 3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells (PubMed:28380054, PubMed:28809393, PubMed:29087293, PubMed:29033321, PubMed:29360036). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability (PubMed:29360036). Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (PubMed:29033321). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease (PubMed:29033321). Required for both spermatogenesis and oogenesis (PubMed:28809393, PubMed:29033321).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226474 | Ythdc2 (tGFP-tagged) - Mouse YTH domain containing 2 (Ythdc2), (10ug) |
CNY 11,880.00 |
|
| MR226474 | Ythdc2 (Myc-DDK-tagged) - Mouse YTH domain containing 2 (Ythdc2) |
CNY 9,888.00 |
|
| MR226474L3 | Lenti ORF clone of Ythdc2 (Myc-DDK-tagged) - Mouse YTH domain containing 2 (Ythdc2) |
CNY 12,730.00 |
|
| MR226474L4 | Lenti ORF clone of Ythdc2 (mGFP-tagged) - Mouse YTH domain containing 2 (Ythdc2) |
CNY 12,730.00 |
