Rock2 (NM_009072) Mouse Untagged Clone

CAT#: MC224381

Rock2 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug)



  "NM_009072" in other vectors (4)

CNY 17,860.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms B230113H15Rik; mKIAA0619; Rho-kinase; Rock-II; Rock2m; ROKalpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224381 representing NM_009072
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCGGGCG
CCGGCCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAGAGTCTGCT
GGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGCTTTGAGGAAAAATAAAAATATAGATAAC
TTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCGGAAGACTATGATG
TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCATAAGGCATCACAGAAGGT
TTATGCAATGAAGCTTCTTAGTAAATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAA
AGAGATATTATGGCCTTTGCCAACAGTCCCTGGGTAGTTCAGCTCTTCTGTGCCTTTCAAGATGACAGGT
ACCTGTACATGGTGATGGAGTACATGCCAGGCGGAGACCTCGTCAACCTTATGAGTAATTATGATGTACC
TGAAAAGTGGGCCAAGTTTTACACTGCTGAAGTAGTGCTTGCACTGGATGCAATACACTCCATGGGCTTA
ATACACAGAGATGTGAAGCCTGATAACATGCTATTGGATAAACATGGACATCTAAAGTTAGCAGATTTTG
GCACATGTATGAAAATGGATGAAACAGGCATGGTGCATTGCGACACAGCAGTTGGAACTCCCGACTACAT
ATCGCCCGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGGCGAGAATGTGATTGGTGGTCTGTA
GGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTTTACGCAGATTCACTTGTAGGAACCTACA
GCAAAATTATGGATCATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAA
TCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGGGTAGAAGAAATCAAACAG
CATCCTTTCTTTAAGAATGATCAATGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTACCTG
AGCTCAGCAGTGACATAGACAGCAGCAACTTTGACGACATTGAGGATGACAAAGGCGATGTAGAGACCTT
CCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTATAGGATTTACCTACTTTAGAGAAAATTTG
TTATTAAGTGACTCTCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGG
AGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTGCAAGCCAAGGAGGAGCTGGA
ACAGAAGTGCAAATCTATTAATACTCGCCTAGAGAAAACAGCAAAGGAGCTAGAAGAAGAGATTACCTTA
CGGAAAAGTGTGGAATCAACATTAAGACAGCTGGAAAGAGAAAAGGCCCTTCTTCAGCACAAAAATGCAG
AGTATCAGCGGAAAGCTGATCATGAAGCCGACAAGAAGCGGAACTTGGAAAATGATGTTAACAGCTTAAA
AGATCAACTTGAAGATCTGAAGAAAAGGAACCAGAGCTCTCAGATATCCACTGAGAAGGTCAATCAGCTC
CAGAAACAACTGGATGAAGCTAATGCTTTGCTGAGAACAGAGTCTGATACTGCAGCACGGTTAAGAAAAA
CCCAGGCAGAAAGTTCCAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG
CCTGCTTGAGACTGCCAAGTTAAAACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTTTAGAATCTGAA
AGAAGGGACCGGACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATCTGGGTTGGAAGAAG
ATTTGAAGACTGGCAAAGCCTTACTAGCAAAAGTAGAGCTGGAGAAGAGACAGCTGCAGGAGAAGCTCAC
TGACTTAGAGAAGGAAAAAAGCAACATGGAAATAGATATGACATATCAACTGAAAGTTATACAGCAGAGT
TTAGAACAAGAAGAAGCTGAACACAAGACCACAAAAGCACGGCTAGCGGATAAAAATAAGATCTATGAAT
CCATTGAAGAAGCTAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTGTTGGAAGAGAGGTCTTTAAA
GCAGAAAGTGGAGAACCTGCTCCTGGAAGCCGAGAAAAGATGCTCTATATTGGACTGTGACCTCAAACAG
TCTCAGCAGAAGCTAAATGAGCTGCTGAAGCAGAAAGATGTGCTGAACGAGGATGTTAGAAATTTGACAT
TAAAAATAGAGCAGGAAACTCAGAAGCGTTGTCTTATGCAAAATGACCTGAAGATGCAGACCCAACAAGT
TAACACCCTAAAAATGTCAGAAAAGCAAATAAAACAAGAAAATAATCATCTTATGGAAATGAAAATGAAC
TTGGAAAAGCAAAATACTGAACTTCGAAAAGAGCGGCAGGATGCAGATGGGCAGATGAAGGAACTGCAAG
ACCAACTTGAAGCAGAGCAGTATTTCTCAACCCTCTATAAAACACAAGTTAGAGAACTTAAAGAAGAAAA
TGAAGAGAAGACAAAACTTTGTAAAGAGTTGCAACAGAAGAAGCAGGATTTACAGGATGAAAGAGACTCT
TTGGCTGCCCAGCTGGAAATTACCCTTACCAAAGCAGATTCTGAGCAGCTGGCTCGTTCCATTGCTGAAG
AACAATACTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAGATCAAAGAGATGATGGCTAGACA
CAAACAAGAACTTACTGAAAAGGATACTACAATTGCATCTCTTGAAGAAACAAATAGGACACTAACTAGC
GATGTTGCAAATCTTGCAAATGAGAAAGAAGAATTAAACAACAAGCTGAAAGATAGCCAAGAACAACTGT
CCAAGTTGAAAGATGAAGAGATGAGTGCAGCAGCTATTAAAGCACAGTTTGAGAAGCAGCTTCTGAATGA
GAGAACTCTCAAGACTCAAGCTGTGAATAAGTTGGCGGAGATCATGAATCGAAAAGAACCTGTCAAGCGT
GGTAGTGACACAGATGTGCGAAGAAAAGAGAAGGAAAACAGAAAGCTACATATGGAGCTTAAGTCTGAAC
GTGAAAAATTGACACAACAGATGATCAAATATCAGAAAGAACTGAATGAAATGCAGGCTCAAATAGCTGA
AGAGAGCCAAATTCGAATAGAACTCCAGATGACCCTGGACAGTAAAGACAGCGACATTGAACAGCTGCGG
TCACAGCTCCAGGCCTTGCATATTGGTATGGACAGTTCCAGTATAGGCAGTGGACCAGGCGATGCTGAGC
CTGATGATGGATTTCCAGAATCAAGATTAGAAGGATGGTTGTCATTGCCTGTGCGCAACAACACTAAAAA
GTTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGCAAGAAGATTCTTTTTTATGACAGCGAACAAGAT
AAAGAGCAGTCCAACCCTTACATGGTGTTAGACATAGACAAGCTATTTCACGTCCGACCTGTTACCCAGA
CAGATGTATACAGAGCAGATGCTAAAGAAATTCCAAGGATATTTCAGATTCTATATGCCAATGAAGGAGA
AAGTAAGAAGGAACCAGAGTTTCCAGTGGAACCAGTGGGAGAAAAATCTAATTATATTTGTCACAAAGGA
CATGAGTTTATTCCTACTCTTTATCACTTCCCAACCAACTGTGAGGCATGTATGAAGCCGCTGTGGCACA
TGTTTAAGCCTCCTCCTGCTCTAGAGTGCCGTAGGTGCCACATTAAATGTCATAAAGATCACATGGACAA
GAAGGAAGAGATTATAGCGCCCTGCAAAGTTTATTATGATATATCGTCGGCAAAGAATCTGTTGTTATTA
GCAAATTCAACAGAAGAACAGCAGAAATGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCTCCAG
CTCCAGACCCTTTTGCCCGATCATCCCCTAGAACTTCAATGAAAATACAACAAAACCAGTCCATTCGGCG
GCCCAGTCGACAGCTTGCTCCAAACAAGCCAAGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009072
Insert Size 4167 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009072.2, NP_033098.2
RefSeq Size 8012 bp
RefSeq ORF 4167 bp
Locus ID 19878
UniProt ID P70336
Gene Summary Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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