United States
Germany
Japan
United Kingdom
China
Rock2 (NM_009072) Mouse Untagged Clone
CAT＃: MC224381
Rock2 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug)
CNY 17,860.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | B230113H15Rik; mKIAA0619; Rho-kinase; Rock-II; Rock2m; ROKalpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224381 representing NM_009072
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCGGGCG CCGGCCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAGAGTCTGCT GGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGCTTTGAGGAAAAATAAAAATATAGATAAC TTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCGGAAGACTATGATG TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCATAAGGCATCACAGAAGGT TTATGCAATGAAGCTTCTTAGTAAATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAA AGAGATATTATGGCCTTTGCCAACAGTCCCTGGGTAGTTCAGCTCTTCTGTGCCTTTCAAGATGACAGGT ACCTGTACATGGTGATGGAGTACATGCCAGGCGGAGACCTCGTCAACCTTATGAGTAATTATGATGTACC TGAAAAGTGGGCCAAGTTTTACACTGCTGAAGTAGTGCTTGCACTGGATGCAATACACTCCATGGGCTTA ATACACAGAGATGTGAAGCCTGATAACATGCTATTGGATAAACATGGACATCTAAAGTTAGCAGATTTTG GCACATGTATGAAAATGGATGAAACAGGCATGGTGCATTGCGACACAGCAGTTGGAACTCCCGACTACAT ATCGCCCGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGGCGAGAATGTGATTGGTGGTCTGTA GGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTTTACGCAGATTCACTTGTAGGAACCTACA GCAAAATTATGGATCATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAA TCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGGGTAGAAGAAATCAAACAG CATCCTTTCTTTAAGAATGATCAATGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTACCTG AGCTCAGCAGTGACATAGACAGCAGCAACTTTGACGACATTGAGGATGACAAAGGCGATGTAGAGACCTT CCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTATAGGATTTACCTACTTTAGAGAAAATTTG TTATTAAGTGACTCTCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGG AGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTGCAAGCCAAGGAGGAGCTGGA ACAGAAGTGCAAATCTATTAATACTCGCCTAGAGAAAACAGCAAAGGAGCTAGAAGAAGAGATTACCTTA CGGAAAAGTGTGGAATCAACATTAAGACAGCTGGAAAGAGAAAAGGCCCTTCTTCAGCACAAAAATGCAG AGTATCAGCGGAAAGCTGATCATGAAGCCGACAAGAAGCGGAACTTGGAAAATGATGTTAACAGCTTAAA AGATCAACTTGAAGATCTGAAGAAAAGGAACCAGAGCTCTCAGATATCCACTGAGAAGGTCAATCAGCTC CAGAAACAACTGGATGAAGCTAATGCTTTGCTGAGAACAGAGTCTGATACTGCAGCACGGTTAAGAAAAA CCCAGGCAGAAAGTTCCAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG CCTGCTTGAGACTGCCAAGTTAAAACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTTTAGAATCTGAA AGAAGGGACCGGACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATCTGGGTTGGAAGAAG ATTTGAAGACTGGCAAAGCCTTACTAGCAAAAGTAGAGCTGGAGAAGAGACAGCTGCAGGAGAAGCTCAC TGACTTAGAGAAGGAAAAAAGCAACATGGAAATAGATATGACATATCAACTGAAAGTTATACAGCAGAGT TTAGAACAAGAAGAAGCTGAACACAAGACCACAAAAGCACGGCTAGCGGATAAAAATAAGATCTATGAAT CCATTGAAGAAGCTAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTGTTGGAAGAGAGGTCTTTAAA GCAGAAAGTGGAGAACCTGCTCCTGGAAGCCGAGAAAAGATGCTCTATATTGGACTGTGACCTCAAACAG TCTCAGCAGAAGCTAAATGAGCTGCTGAAGCAGAAAGATGTGCTGAACGAGGATGTTAGAAATTTGACAT TAAAAATAGAGCAGGAAACTCAGAAGCGTTGTCTTATGCAAAATGACCTGAAGATGCAGACCCAACAAGT TAACACCCTAAAAATGTCAGAAAAGCAAATAAAACAAGAAAATAATCATCTTATGGAAATGAAAATGAAC TTGGAAAAGCAAAATACTGAACTTCGAAAAGAGCGGCAGGATGCAGATGGGCAGATGAAGGAACTGCAAG ACCAACTTGAAGCAGAGCAGTATTTCTCAACCCTCTATAAAACACAAGTTAGAGAACTTAAAGAAGAAAA TGAAGAGAAGACAAAACTTTGTAAAGAGTTGCAACAGAAGAAGCAGGATTTACAGGATGAAAGAGACTCT TTGGCTGCCCAGCTGGAAATTACCCTTACCAAAGCAGATTCTGAGCAGCTGGCTCGTTCCATTGCTGAAG AACAATACTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAGATCAAAGAGATGATGGCTAGACA CAAACAAGAACTTACTGAAAAGGATACTACAATTGCATCTCTTGAAGAAACAAATAGGACACTAACTAGC GATGTTGCAAATCTTGCAAATGAGAAAGAAGAATTAAACAACAAGCTGAAAGATAGCCAAGAACAACTGT CCAAGTTGAAAGATGAAGAGATGAGTGCAGCAGCTATTAAAGCACAGTTTGAGAAGCAGCTTCTGAATGA GAGAACTCTCAAGACTCAAGCTGTGAATAAGTTGGCGGAGATCATGAATCGAAAAGAACCTGTCAAGCGT GGTAGTGACACAGATGTGCGAAGAAAAGAGAAGGAAAACAGAAAGCTACATATGGAGCTTAAGTCTGAAC GTGAAAAATTGACACAACAGATGATCAAATATCAGAAAGAACTGAATGAAATGCAGGCTCAAATAGCTGA AGAGAGCCAAATTCGAATAGAACTCCAGATGACCCTGGACAGTAAAGACAGCGACATTGAACAGCTGCGG TCACAGCTCCAGGCCTTGCATATTGGTATGGACAGTTCCAGTATAGGCAGTGGACCAGGCGATGCTGAGC CTGATGATGGATTTCCAGAATCAAGATTAGAAGGATGGTTGTCATTGCCTGTGCGCAACAACACTAAAAA GTTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGCAAGAAGATTCTTTTTTATGACAGCGAACAAGAT AAAGAGCAGTCCAACCCTTACATGGTGTTAGACATAGACAAGCTATTTCACGTCCGACCTGTTACCCAGA CAGATGTATACAGAGCAGATGCTAAAGAAATTCCAAGGATATTTCAGATTCTATATGCCAATGAAGGAGA AAGTAAGAAGGAACCAGAGTTTCCAGTGGAACCAGTGGGAGAAAAATCTAATTATATTTGTCACAAAGGA CATGAGTTTATTCCTACTCTTTATCACTTCCCAACCAACTGTGAGGCATGTATGAAGCCGCTGTGGCACA TGTTTAAGCCTCCTCCTGCTCTAGAGTGCCGTAGGTGCCACATTAAATGTCATAAAGATCACATGGACAA GAAGGAAGAGATTATAGCGCCCTGCAAAGTTTATTATGATATATCGTCGGCAAAGAATCTGTTGTTATTA GCAAATTCAACAGAAGAACAGCAGAAATGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCTCCAG CTCCAGACCCTTTTGCCCGATCATCCCCTAGAACTTCAATGAAAATACAACAAAACCAGTCCATTCGGCG GCCCAGTCGACAGCTTGCTCCAAACAAGCCAAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009072 |
| Insert Size | 4167 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009072.2, NP_033098.2 |
| RefSeq Size | 8012 bp |
| RefSeq ORF | 4167 bp |
| Locus ID | 19878 |
| UniProt ID | P70336 |
| Gene Summary | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222994 | Rock2 (tGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug) |
CNY 11,096.00 |
|
| MR222994 | Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 9,496.00 |
|
| MR222994L3 | Lenti ORF clone of Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 14,160.00 |
|
| MR222994L4 | Lenti ORF clone of Rock2 (mGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 14,160.00 |
