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Rock2 (NM_009072) Mouse Tagged Lenti ORF Clone
CAT＃: MR222994L3
- LentiORF®
Lenti ORF clone of Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2)
"NM_009072" in other vectors (4)
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CNY 14,160.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B230113H15Rik; mKIAA0619; Rho-kinase; Rock-II; Rock2m; ROKalpha |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR222994).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009072 |
| ORF Size | 4164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009072.2, NP_033098.2 |
| RefSeq Size | 8012 bp |
| RefSeq ORF | 4167 bp |
| Locus ID | 19878 |
| UniProt ID | P70336 |
| Gene Summary | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224381 | Rock2 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug) |
CNY 17,860.00 |
|
| MG222994 | Rock2 (tGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug) |
CNY 11,096.00 |
|
| MR222994 | Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 9,496.00 |
|
| MR222994L4 | Lenti ORF clone of Rock2 (mGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 14,160.00 |
