United States
Germany
Japan
United Kingdom
China
Drosha (NM_001130149) Mouse Untagged Clone
CAT＃: MC224355
Drosha (untagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1, (10ug)
CNY 17,580.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224355 representing NM_001130149
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGCAATACGTGTCATAGAATGTCGTACCACCCAGGACGAGGGTGTCCCCGGGGCCGAGGAGGAC ACGGAGCCAGACCTTCAGCACCAGCTTTCAGACCCCAAAACCTGCGACTTCTTCATCCCCAGCAGCCGCC TGCGCAGTATCAATATGAGCCTCCGAGCGCCCCCTCTTCCTCCTACTCGAACTCTCAGGCCCCCAGCTTT ATGCCCCCACGGCCAGACTTTGTCCCTTACCCTCCCCCAGCGGCGCCGTCTGCCCAAGGGCCTCTCCCTC CCTGCCCAGTGAGGCCGCCTTACCCCAACCACCAGATGAGACACCCTTTCCCGGTGCCTCCCTGTTTTCC ACCCATGCCCCCTCCGATGCCTTGTCCCAATAACCCGCCTGCCTCCGGAGCACCTCCCGGACAAGGCACT TTCCCCTTCATGGTGCCCCCTCCTTCCATGCCCCACCCTCCGCCCCCGCCCGTCATGCCGCAGCAGGTTA ATTACCAGTACCCCCCTGGGTACTCGCACAGCTTCCCACCGCCCGGCTTCAACAGTTACCAGAACAACTC CAGCTCTTTCCCACCCAGTGCTAACAGCAGCAGCACTCCTCATTTTCGACACCTCCCACCATACTCACTC CCAAAGGCTCAGAATGAGAGGCGGTCCCCAGAAAGGCTCAAGCACTACGACGACCACAGGCACCGAGATC ACAGTCACGGGCGAGGCGAGAGGCATCGGTCCCTGGAGCGCAGGGAGCGCGGCCGCAGCCCTGAAAGGAG AAGACCTGAGAGCCGCTACCGCTCAGACTATGATCGGGGGAGAACGCCACCGCCTCGCCACCGCAGCTAT GAAAGGAGCAGAGAGCGGGATCGAGAGAGACACAGGCACCGGGAGGCCCGCAGATCACCGTCTCTAGAAA GGTCCTACAAGAAAGAGTATAAGAGATCTGGAAGGAGTTACGCTTTACCAGTTGCTCCTGAGCCCGCTGG GTGCACACCAGAGTTGCCTGGGGAGATGATTAAAACTACAGAGTCTTGGGCCCCGCCCCCGGAGAATGTG AATCATCGTTCTCCAAGCAGGGAGAAGAAGAGAGCTCGTTGGGAGGAGGAAAAAGACAGATGGAGCGACA GCCAGGGCTCTGGCAAAGAGAAGAACTACACGTCCATCAAAGAGAAAGAGGCAGAGGAGGTGCCTCCAGA GAAGACGGAGGAGGAGGAGGAAGAGCTCCTTAAGCCTGTGTGGATTCGCTGTACACATTCGGAAAGCTAT TACTCCAGTGACCCAATGGATCAGGTGGGAGACTCGACTGTCGTAGGGACAAGCAGGCTCCGTGATTTGT ATGACAAATTTGAGGAAGAATTGGGGAATAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCGTGGGAGCC TCCGAAGACAAAGCTGGATGAAGATTTAGAGAGTTCCAGTGAGTCTGAATGTGAGACTGACGACGACAGC ACCTGTTCGAGCAGCTCGGACTCGGAGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACC CTGACCGGCTTCATGATGAACTCTGGTACAACGACCCAGGCCAGATGAACGATGGACCGCTTTGCAAATG CAGTGCAAAAGCCAGACGCACAGGAATCCGCCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGC CGTCCGATGACCAACAACGCTGGCCGGCTTTTCCACTATCGGATCACCGTCTCCCCGCCTACCAACTTCT TAACTGACAGGCCAACAGTCATAGAATATGATGACCACGAGTATATTTTTGAAGGATTTTCTATGTTTGC ACATGCTCCTCTGACCAATATTCCACTGTGTAAAGTGATTCGATTCAACATAGACTACACGATTCATTTT ATCGAAGAGATGATGCCTGAGAATTTTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAG ATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCGAG ATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGCAAAGAAGTGTTATCCATGCACCAG ATCCTTCTCTACCTGCTGCGCTGCAGCAAGGCTCTGGTGCCCGAGGAGGAGATTGCCAACATGCTCCAGT GGGAGGAGCTCGAGTGGCAGAAATACGCAGAGGAGTGCAAAGGCATGATCGTTACCAACCCTGGGACGAA ACCGAGCTCTGTCCGCATTGATCAGCTGGATCGTGAACAGTTCAACCCCGAAGTGATCACTTTCCCGATT ATCGTTCACTTTGGGATCCGCCCCGCACAGTTGAGTTATGCAGGAGACCCACAGTATCAGAAATTATGGA AAAGCTATGTGAAACTTCGCCACCTCCTAGCAAACAGTCCCAAAGTCAAACAAACTGACAAGCAGAAGCT GGCTCAGAGGGAGGAAGCGCTCCAGAAAATACGGCAGAAGAACACGATGCGGCGAGAAGTCACCGTGGAG CTGAGTAGCCAAGGATTCTGGAAAACTGGCATTCGTTCTGATGTCTGTCAGCATGCAATGATGCTGCCTG TTCTGACCCATCACATCCGGTACCATCAATGCTTAATGCACCTGGACAAGTTGATAGGATATACTTTCCA AGACCGTTGTCTGCTCCAGCTTGCCATGACTCATCCGAGTCACCATTTAAATTTTGGAATGAATCCTGAC CATGCCAGGAATTCTTTGTCTAACTGTGGAATTCGACAACCCAAATATGGAGACAGAAAAGTTCATCACA TGCACATGCGGAAAAAAGGAATTAATACCCTAATAAATATCATGTCACGCCTTGGCCAAGATGATCCAAC TCCTTCAAGGATTAATCACAACGAAAGGTTGGAGTTCCTGGGGGATGCTGTTGTTGAGTTTCTGACCAGT GTCCACCTGTACTACCTGTTTCCTAGCTTGGAGGAAGGGGGCTTGGCGACCTATCGGACCGCCATTGTTC AGAATCAGCACCTCGCCATGCTTGCAAAGAAACTTGAACTGGATCGATTTATGCTATATGCCCATGGGCC CGACCTATGTAGAGAATCAGATCTCCGCCATGCGATGGCCAATTGTTTTGAAGCCTTGATAGGAGCTGTT TACTTGGAGGGGAGCCTAGAGGAAGCCAAACAGTTATTTGGACGCTTACTCTTTAATGATCCGGACCTTC GAGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACGGATCGGCAACTTAT TGAAACTTCCCCAGTTCTACAGAAACTTACGGAGTTTGAAGAAGCAATTGGAGTGATCTTCACTCACGTC CGGCTTCTGGCGAGGGCGTTCACACTGAGAACCGTGGGCTTTAACCACCTGACCCTAGGCCACAATCAGA GGATGGAATTTCTGGGCGACTCCATAATGCAGCTGGTGGCCACAGAGTACTTGTTCATTCATTTCCCGGA CCATCACGAAGGACACTTGACGTTGTTACGAAGTTCCTTAGTGAACAACAGAACGCAAGCCAAGGTAGCA GAGGAACTGGGCATGCAGGAGTATGCCATCACCAACGACAAAACCAAGAGACCCGTGGCCCTAAGAACCA AGACTTTGGCAGACCTTTTGGAATCATTTATCGCAGCGCTGTACATCGACAAGGACCTGGAATATGTCCA CACTTTCATGAACGTCTGCTTCTTTCCCCGGCTGAAGGAGTTCATTCTGAATCAGGATTGGAACGACCCC AAGTCGCAGCTGCAGCAGTGTTGTCTGACCCTGAGGACAGAAGGGAAAGAGCCTGACATCCCCTTATACA AGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTACACTGTGGCTGTTTATTTCAAGGGAGAAAG GATAGGCTGTGGGAAAGGACCAAGCATTCAGCAGGCGGAGATGGGAGCAGCAATGGATGCACTGGAGAAA TATAACTTTCCCCAGATGGCCCATCAGAAGCGGTTCATTGAGCGGAAATACAGACAAGAGTTAAAGGAAA TGAGGTGGGAAAGAGAGCATCAGGAGAGAGAGCCGGAGGAGGCTGAAGACATCAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130149 |
| Insert Size | 4122 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130149.1, NP_001123621.1 |
| RefSeq Size | 4584 bp |
| RefSeq ORF | 4122 bp |
| Locus ID | 14000 |
| UniProt ID | Q5HZJ0 |
| Gene Summary | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227001 | Drosha (tGFP-tagged) - Mouse ribonuclease III nuclear (Rnasen) transcript variant 1, (10ug) |
CNY 13,300.00 |
|
| MR227001 | Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 9,392.00 |
|
| MR227001L3 | Lenti ORF clone of Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 13,970.00 |
|
| MR227001L4 | Lenti ORF clone of Drosha (mGFP-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 13,970.00 |
