Kdm3a (NM_173001) Mouse Untagged Clone

CAT#: MC224246

Kdm3a (untagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1, (10ug)



  "NM_173001" in other vectors (5)

CNY 17,010.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224246 representing NM_173001
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTCACGCTCGGAGAAAGTTGGCCAGTATTGGTGGGGAAGCGATTCCTCAGTCTGTCCGCAGCCG
AAGGCAACGAAGGCGGCCAGGACAACTGGGACTTGGAGCGCGTTGCCGAGTGGCCCTGGCTGTCGGGGAC
CATTCGAGCTGTTTCCCACACCGACGTTACTAAGAAAGACTTGAAGGTGTGTGTGGAGTTTGATGGGGAG
TCTTGGAGAAAGAGAAGATGGATAGATGTCTACAGCCTTCAGAGAAAAGCATTTTTAGTAGAGCATAACC
TGGTTTTGGCAGAACGAAAATCACCTGAAGTTCCTGAGCAAGTTATTCAGTGGCCTGCAATAATGTACAA
ATCTCTTCTAGACAAAGCTGGCTTGGGAGCCATAACTTCTGTTCGGTTTCTTGGAGATCAACAAAGTGTA
TTTGTTTCCAAAGACCTTTTGAAACCTATACAGGATGTTAACAGTCTTCGGCTTTCCCTTACTGATAATC
AGACAGTCAGTAAGGAATTTCAAGCTTTGATTGTAAAACATTTGGATGAAAGCCATCTTTTACAAGGTGA
CAAGAACCTTGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACT
GTTGTACATGGAAACCCATCATCCAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG
TCGACCCAGCACTGATTCATGTTGAAGTTGTACATGACAACTTTGTGACATGTGGTAATTCTACAAGAAC
TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAACGGAAGTTCGGTTTCTAAACAAGCAAAATCTTGTTCT
GAGGCCTCTCCCAGTATGTGTCCTGTACAGTCTGTTCCCACAACAGTGTTTAAGGAGATCCTGCTTGGCT
GTACTGCAGCAACTCCATCTAGCAAGGACCCAAGACAGCAAAATACTCCCCAGGCAGCCAATTCTCCACC
TAACATTGGAGCAAAACTTCCTCAAGGATGTCATAAGCAGAACTTACCAGAAGAACTTTCTTCCTGTCTA
AACACAAAACCTGAAGTACCGAGAACAAAACCAGATGTCTGCAAAGAAGGATTACTTTCTTCAAAATCTT
CTCAGGTTGGAGCTGGAGACTTGAAAATTCTGAGTGAGCCCAAAGGTAGCTGTATCCAGCCTAAAACAAA
CACTGATCAGGAGAGCAGACTGGAGTCTGCTCCACAGCCAGTCACTGGCCTTCCAAAGGAGTGCTTGCCT
GCAAAGACTTCCTCTAAGGCAGAACTGGACATTGCCACCACTCCTGAACTGCAGAAGCATCTAGAACATG
CAGCTTCCACATCCGATGACCTTTCAGATAAGCCAGAAGTGAAAGCAGGTGTCACTAGCCTTAATAGTTG
TGCAGAAAAGAAGGTCGAACCTTCACATTTAGGTTCCCAGTCACAGAATTTAAAGGAAACTTCAGTAAAA
GTAGATAATGAAAGCTGTTGTACAAGAAGCAGTAATAAAACCCAGACTCCCCCAGCCCGGAAGTCAGTTT
TGACAGACCCAGATAAAGTCAGGAAGCTGCAGCAGAGCGGAGAGGCCTTTGTTCAGGATGACTCCTGTGT
TAACATCGTGGCACAGCTGCCCAAGTGTCGGGAGTGTCGACTAGACAGCCTGCGCAAGGATAAGGACCAG
CAGAAGGACTCTCCTGTGTTTTGTCGCTTTTTCCACTTCAGGAGATTACAATTCAACAAGCATGGTGTGT
TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAGTGAAGCGATTGGCTTGTGGCTGCCTTTGAC
CAAAAATGTTGTGGGGACTGATTTGGACACAGCAAAATATATCCTGGCCAATATTGGAGACCACTTCTGT
CAAATGGTGATTTCTGAGAAGGAAGCTATGTCGACTATTGAGCCACACAGACAGGTTGCTTGGAAACGAG
CTGTCAAAGGAGTTAGAGAAATGTGTGATGTGTGTGACACAACCATTTTCAACCTGCACTGGGTGTGCCC
TCGGTGTGGGTTTGGAGTATGTGTAGATTGCTACCGGATGAAGAGGAAGAACTGCCAACAGGGTGCTGCC
TACAAGACTTTCTCTTGGATAAGGTGTGTGAAGAGTCAGATACATGAGCCTGAGAACCTGATGCCCACAC
AGATTATTCCTGGCAAAGCCCTCTACGATGTTGGAGACATTGTGCATTCTGTCAGAGCAAAATGGGGCAT
AAAGGCCAATTGTCCCTGCTCCAACAGGCAGTTCAAGCTCTTCTCAAAGCCAGCCTTAAAGGAAGACCTG
AAACAGACATCCTTGTCTGGAGAAAAACCAACTCTTGGGACCATGGTCCAGCAAAGTTCCCCTGTTTTGG
AGCCAGTGGCTGTGTGCGGGGAAGCAGCCTCCAAGCCAGCCAGCAGCGTGAAGCCCACCTGTCCCACCAG
CACTTCACCTTTAAACTGGCTAGCTGACCTTACCAGTGGGAATGTCAACAAGGAGAATAAGGAAAAACAG
CTGACTATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCTTGCCCCCTCTGAACAAGCCCAGCA
CAGTCCTCCATACTTTTAACAGCACCATCTTGACACCTGTGAGCAACAATAATTCAGGTTTCCTTAGAAA
TCTTTTGAATTCATCCACAGCAAAGACAGAAAATGGATTGAAAAACACACCCAAAATTCTTGATGACATC
TTTGCCTCTTTGGTGCAAAACAAGACTTCTTCTGATTCATCCAAGAGGCCTCAAGGACTGACAATCAAGC
CTAGCATTCTTGGCTTTGACACTCCTCACTACTGGCTGTGTGACAACCGCCTGCTGTGCTTGCAAGACCC
CAACAATAAGAGCAATTGGAATGTTTTTAGGGAATGCTGGAAACAAGGGCAGCCAGTGATGGTGTCGGGC
GTGCATCATAAATTAAACACTGAACTCTGGAAACCCGAGTCCTTCAGAAAAGAGTTTGGTGAGCAGGAAG
TAGACCTAGTCAATTGTAGGACCAATGAAATCATCACTGGAGCCACAGTCGGAGACTTCTGGGATGGATT
TGAAGATGTTCCAAACCGTTTGAAAAACGACAAAGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGG
CCGCCAGGAGAAGACTTCAGAGACATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGC
CTGAGTACACCAGGCGAGATGGCAAACTGAACCTGGCTTCCAGACTGCCAAACTACTTTGTACGGCCAGA
CCTGGGCCCCAAGATGTACAATGCTTATGGATTGATCACTCCAGAGGATCGGAAATATGGGACCACAAAT
CTTCACTTAGATGTATCTGATGCAGCCAATGTCATGGTTTATGTGGGAATTCCCAAAGGACAGTGTGAAC
AAGAAGAAGAAGTCCTTAGAACCATCCAAGATGGAGATTCTGATGAACTCACAATCAAGAGATTTATTGA
AGGAAAAGAGAAGCCAGGAGCCCTTTGGCACATATATGCTGCTAAAGACACAGAGAAGATAAGAGAATTC
CTTAAAAAGGTATCAGAGGAGCAGGGTCAAGACAACCCTGCAGACCATGACCCTATCCACGATCAGAGCT
GGTATTTAGACCGATCGCTGAGAAAGCGCCTCTATCAAGAGTACGGCGTGCAAGGCTGGGCTATTGTACA
GTTTCTTGGGGATGTGGTGTTTATCCCAGCAGGAGCGCCACATCAGGTTCATAACTTATACAGCTGTATC
AAAGTGGCTGAAGACTTTGTGTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGTT
ACTTGTCACAGACTCATACCAACCATGAAGATAAATTGCAGGTGAAAAATGTTATCTACCATGCAGTGAA
AGATGCAGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTGGGCAAACCTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_173001
Insert Size 3972 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173001.3, NP_766589.1
RefSeq Size 4857 bp
RefSeq ORF 3972 bp
Locus ID 104263
UniProt ID Q6PCM1
Gene Summary Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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