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Kdm3a (NM_173001) Mouse Untagged Clone
CAT＃: MC224246
Kdm3a (untagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1, (10ug)
CNY 17,010.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700105C21Rik; C230043E16Rik; JHDM2a; Jmjd1; Jmjd1a; KDM2A; TGSA; Tsga |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224246 representing NM_173001
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCTCACGCTCGGAGAAAGTTGGCCAGTATTGGTGGGGAAGCGATTCCTCAGTCTGTCCGCAGCCG AAGGCAACGAAGGCGGCCAGGACAACTGGGACTTGGAGCGCGTTGCCGAGTGGCCCTGGCTGTCGGGGAC CATTCGAGCTGTTTCCCACACCGACGTTACTAAGAAAGACTTGAAGGTGTGTGTGGAGTTTGATGGGGAG TCTTGGAGAAAGAGAAGATGGATAGATGTCTACAGCCTTCAGAGAAAAGCATTTTTAGTAGAGCATAACC TGGTTTTGGCAGAACGAAAATCACCTGAAGTTCCTGAGCAAGTTATTCAGTGGCCTGCAATAATGTACAA ATCTCTTCTAGACAAAGCTGGCTTGGGAGCCATAACTTCTGTTCGGTTTCTTGGAGATCAACAAAGTGTA TTTGTTTCCAAAGACCTTTTGAAACCTATACAGGATGTTAACAGTCTTCGGCTTTCCCTTACTGATAATC AGACAGTCAGTAAGGAATTTCAAGCTTTGATTGTAAAACATTTGGATGAAAGCCATCTTTTACAAGGTGA CAAGAACCTTGTTGGTTCAGAAGTAAAAATTTATAGCTTGGACCCATCTACTCAGTGGTTTTCAGCAACT GTTGTACATGGAAACCCATCATCCAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTG TCGACCCAGCACTGATTCATGTTGAAGTTGTACATGACAACTTTGTGACATGTGGTAATTCTACAAGAAC TGGAGCTGTAAAACGCAAGTCTTCTGAGAATAACGGAAGTTCGGTTTCTAAACAAGCAAAATCTTGTTCT GAGGCCTCTCCCAGTATGTGTCCTGTACAGTCTGTTCCCACAACAGTGTTTAAGGAGATCCTGCTTGGCT GTACTGCAGCAACTCCATCTAGCAAGGACCCAAGACAGCAAAATACTCCCCAGGCAGCCAATTCTCCACC TAACATTGGAGCAAAACTTCCTCAAGGATGTCATAAGCAGAACTTACCAGAAGAACTTTCTTCCTGTCTA AACACAAAACCTGAAGTACCGAGAACAAAACCAGATGTCTGCAAAGAAGGATTACTTTCTTCAAAATCTT CTCAGGTTGGAGCTGGAGACTTGAAAATTCTGAGTGAGCCCAAAGGTAGCTGTATCCAGCCTAAAACAAA CACTGATCAGGAGAGCAGACTGGAGTCTGCTCCACAGCCAGTCACTGGCCTTCCAAAGGAGTGCTTGCCT GCAAAGACTTCCTCTAAGGCAGAACTGGACATTGCCACCACTCCTGAACTGCAGAAGCATCTAGAACATG CAGCTTCCACATCCGATGACCTTTCAGATAAGCCAGAAGTGAAAGCAGGTGTCACTAGCCTTAATAGTTG TGCAGAAAAGAAGGTCGAACCTTCACATTTAGGTTCCCAGTCACAGAATTTAAAGGAAACTTCAGTAAAA GTAGATAATGAAAGCTGTTGTACAAGAAGCAGTAATAAAACCCAGACTCCCCCAGCCCGGAAGTCAGTTT TGACAGACCCAGATAAAGTCAGGAAGCTGCAGCAGAGCGGAGAGGCCTTTGTTCAGGATGACTCCTGTGT TAACATCGTGGCACAGCTGCCCAAGTGTCGGGAGTGTCGACTAGACAGCCTGCGCAAGGATAAGGACCAG CAGAAGGACTCTCCTGTGTTTTGTCGCTTTTTCCACTTCAGGAGATTACAATTCAACAAGCATGGTGTGT TGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAGTGAAGCGATTGGCTTGTGGCTGCCTTTGAC CAAAAATGTTGTGGGGACTGATTTGGACACAGCAAAATATATCCTGGCCAATATTGGAGACCACTTCTGT CAAATGGTGATTTCTGAGAAGGAAGCTATGTCGACTATTGAGCCACACAGACAGGTTGCTTGGAAACGAG CTGTCAAAGGAGTTAGAGAAATGTGTGATGTGTGTGACACAACCATTTTCAACCTGCACTGGGTGTGCCC TCGGTGTGGGTTTGGAGTATGTGTAGATTGCTACCGGATGAAGAGGAAGAACTGCCAACAGGGTGCTGCC TACAAGACTTTCTCTTGGATAAGGTGTGTGAAGAGTCAGATACATGAGCCTGAGAACCTGATGCCCACAC AGATTATTCCTGGCAAAGCCCTCTACGATGTTGGAGACATTGTGCATTCTGTCAGAGCAAAATGGGGCAT AAAGGCCAATTGTCCCTGCTCCAACAGGCAGTTCAAGCTCTTCTCAAAGCCAGCCTTAAAGGAAGACCTG AAACAGACATCCTTGTCTGGAGAAAAACCAACTCTTGGGACCATGGTCCAGCAAAGTTCCCCTGTTTTGG AGCCAGTGGCTGTGTGCGGGGAAGCAGCCTCCAAGCCAGCCAGCAGCGTGAAGCCCACCTGTCCCACCAG CACTTCACCTTTAAACTGGCTAGCTGACCTTACCAGTGGGAATGTCAACAAGGAGAATAAGGAAAAACAG CTGACTATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCTTGCCCCCTCTGAACAAGCCCAGCA CAGTCCTCCATACTTTTAACAGCACCATCTTGACACCTGTGAGCAACAATAATTCAGGTTTCCTTAGAAA TCTTTTGAATTCATCCACAGCAAAGACAGAAAATGGATTGAAAAACACACCCAAAATTCTTGATGACATC TTTGCCTCTTTGGTGCAAAACAAGACTTCTTCTGATTCATCCAAGAGGCCTCAAGGACTGACAATCAAGC CTAGCATTCTTGGCTTTGACACTCCTCACTACTGGCTGTGTGACAACCGCCTGCTGTGCTTGCAAGACCC CAACAATAAGAGCAATTGGAATGTTTTTAGGGAATGCTGGAAACAAGGGCAGCCAGTGATGGTGTCGGGC GTGCATCATAAATTAAACACTGAACTCTGGAAACCCGAGTCCTTCAGAAAAGAGTTTGGTGAGCAGGAAG TAGACCTAGTCAATTGTAGGACCAATGAAATCATCACTGGAGCCACAGTCGGAGACTTCTGGGATGGATT TGAAGATGTTCCAAACCGTTTGAAAAACGACAAAGAAAAAGAACCAATGGTGTTGAAACTTAAGGACTGG CCGCCAGGAGAAGACTTCAGAGACATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACTGC CTGAGTACACCAGGCGAGATGGCAAACTGAACCTGGCTTCCAGACTGCCAAACTACTTTGTACGGCCAGA CCTGGGCCCCAAGATGTACAATGCTTATGGATTGATCACTCCAGAGGATCGGAAATATGGGACCACAAAT CTTCACTTAGATGTATCTGATGCAGCCAATGTCATGGTTTATGTGGGAATTCCCAAAGGACAGTGTGAAC AAGAAGAAGAAGTCCTTAGAACCATCCAAGATGGAGATTCTGATGAACTCACAATCAAGAGATTTATTGA AGGAAAAGAGAAGCCAGGAGCCCTTTGGCACATATATGCTGCTAAAGACACAGAGAAGATAAGAGAATTC CTTAAAAAGGTATCAGAGGAGCAGGGTCAAGACAACCCTGCAGACCATGACCCTATCCACGATCAGAGCT GGTATTTAGACCGATCGCTGAGAAAGCGCCTCTATCAAGAGTACGGCGTGCAAGGCTGGGCTATTGTACA GTTTCTTGGGGATGTGGTGTTTATCCCAGCAGGAGCGCCACATCAGGTTCATAACTTATACAGCTGTATC AAAGTGGCTGAAGACTTTGTGTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTCCGTT ACTTGTCACAGACTCATACCAACCATGAAGATAAATTGCAGGTGAAAAATGTTATCTACCATGCAGTGAA AGATGCAGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTGGGCAAACCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173001 |
| Insert Size | 3972 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173001.3, NP_766589.1 |
| RefSeq Size | 4857 bp |
| RefSeq ORF | 3972 bp |
| Locus ID | 104263 |
| UniProt ID | Q6PCM1 |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin (PubMed:17943087). Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform (a). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211928 | Kdm3a (tGFP-tagged) - Mouse jumonji domain containing 1A (Jmjd1a), transcript variant 1 |
CNY 10,550.00 |
|
| MG222266 | Kdm3a (tGFP-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a) transcript variant 1, (10ug) |
CNY 10,550.00 |
|
| MR222266 | Kdm3a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1 |
CNY 9,216.00 |
|
| MR222266L3 | Lenti ORF clone of Kdm3a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1 |
CNY 11,500.00 |
|
| MR222266L4 | Lenti ORF clone of Kdm3a (mGFP-tagged) - Mouse lysine (K)-specific demethylase 3A (Kdm3a), transcript variant 1 |
CNY 11,500.00 |
