Mcm9 (NM_027830) Mouse Untagged Clone

CAT#: MC224169

Mcm9 (untagged) - Mouse minichromosome maintenance complex component 9 (Mcm9), (10ug)



  "NM_027830" in other vectors (4)

CNY 16,630.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9030408O17Rik; Gm235; Mcmdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224169 representing NM_027830
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCAGAGAACTACACGAAATGGAAAATATTGTGACGTGGAACCGGTGTCCCGCTCAAACCCCGCCC
CATGCCTCCGAGACCCGCCCCTCAGACGTCTTGTCCGGCCCAAACCCCGCCTCCAGCTCCCCGAGTCCCG
CCTCTCTCCCTGCTCCCGCCTCCCGCTCGCAGACTCCAGCGTCCGTCCTGGCGCGCGCCCGCCTGCGTCC
GCACCCGGAAGAAGCCCCAGCGGCCGGAAAGTTGAGGCAGTGCGCGGCTCGGGGTCCGCGGGAAGCTCAA
GTCCTTCAGAGGCCGAGCGAGAGCAGCGCGAAGAAGCCTGCGCGCCCCCGCGCAAGGCGGCCCCCTCGAG
CGGCCGCGCGCACGCCCCGCCCCCTCCAACGCCGCGCGGGTCGGGCTGGGGCGACCACGGCCGCAGCGCG
GTCCCGGCGACCAAGACAGTGCGTGTTGAGCCCTACCCACCCTTCAAGATGAATAGTGAGCAGGTCACCC
TGGTGGGTCAGGTGTTTGAGTCCTATGTTTCAGAGTACCATAAGAACGATATTCTTCTGATCCTGAAAGA
AAGAGATGAAGATGCTCACTACCCGGTTGTGGTTAATGCTATGAGCCTTTTCGAGACCAACATGGAAATT
GGGGACTATTTCACCGTGTTCCCCAATGAAGTACTAACAGTTTTTGACAGTGCACTTCGAAGGTCAGCCT
TGGCAATTCTGCAGTCCCTTCCTGAGACGGAGGGGTTATCCATGAAGCAGAATCTTCATGCCAGGATATC
AGGTTTGCCTGTTTGTCCAGAACTGGTCAGGGAACACATTCCCAAAACCAAGGATGTGGGACACTTCTTA
TCTGTCACTGGGACAGTGATCCGAACGAGTCTGGTGAAGGTCTTGGAGTTCGAGCGGGATTACATGTGTA
ACAAATGCAAGCATGTGTTCATGGTGGAGGCAGACTTCGAGCAGTATTACACCTTCAGTCGGCCATCGTC
ATGTCCAAGTTTAGCCAGCTGTGACTCCTCAAAATTCTCTTGCCTCTCAGACTTGTCTTCATCTCCAGCC
AGATGTCGGGATTACCAGGAAATCAAAATTCAGGAGCAGGTGCAAAGGCTGTCTGTTGGAAGTATCCCAC
GGTCTATGAAAGTTATTCTGGAAGATGACCTAGTTGACAGTTGCAAATCTGGAGATGACCTCACCATCTA
TGGGGTTGTAATGCAACGGTGGAAACCCTTTCAGCGAGATGTACGCTGTGAAGTTGAGATTGTCTTGAAA
GCCAACTATGTCCAAGTGAATAATGAGCAATCCTCGGGGATGGTCATGGATGAGGACACTCGAAAAGAAT
TTGAAGACTTCTGGGAACACTATAAGAGTGACCCCTTTGCAGGGAGGAATGAAATATTGGCCAGCTTGTG
TCCTCAAGTTTTTGGGATGTATCTAGTGAAGCTTGCTGTGGCCATGGTACTGGCTGGTGGAATTCAAAGA
ACTGATGCTGCAGGAACCAGGGTTAGAGGGGAATCTCACCTTTTATTGGTTGGGGATCCTGGCACAGGGA
AATCACAATTCCTTAAATATGCAGCAAAGATTACCCCAAGGTCCGTTTTGACCACAGGAATTGGATCTAC
TAGTGCAGGTCTGACCGTGACAGCTGTCAAAGACTCAGGAGAATGGAATCTAGAGGCTGGGGCTTTGGTG
CTTGCAGATGCTGGTCTCTGCTGTATTGACGAATTTAACAGCCTCAAAGAACATGACAGGACAAGCATCC
ATGAAGCAATGGAGCAACAAACCATAAGTGTTGCTAAGGCTGGCCTTGTTTGTAAGCTGAACACAAGGAC
CACCATCCTGGCAGCAACTAACCCCAAAGGCCAGTATGACCCCAAGGAGTCTGTGTCTGTGAACATTGCC
CTCGGGAGCCCACTCTTAAGTCGATTTGACCTTGTCCTGGTTTTGCTCGACACTAGGAATGAAGACTGGG
ATCGTATCATTTCTTCCTTTATCTTAGAAAATAAAGGTTATCCAAGCAAATCAGAGAATCTGTGGAGCAT
GGAGAAGATGAAAACCTACTTCTGCCTCATTCGGAATCTCCACCCCACACTGTCTGAAGTGAGCAATCAA
GTCCTCCTTCGATACTACCAAATGCAAAGGCAGAGTGATTCCCGGAATGCAGCCCGGACAACCATCCGCC
TGTTAGAAAGCTTGATCCGATTAGCAGAAGCTCACGCTCGCCTGATGTTCCGCAGTGCCGTGACTCTGGA
AGATGCCGTTACAGCTGTATCTGTGATGGAGTCTTCAATGCAGGGAGGTGCTCTGCTAGGAGGTGTGAAT
GCTCTCCACACTTCCTTCCCTGAAAACCCCCGTGCACAGTACCAGAGGCAGTGTGAACTCATTCTGGAAA
AGCTGGAACTCCAGGGCCTCTTGCAGGAAGAACTTCGAAGACTGGAAAGGTTACAGAATGAGAGTGTACA
CCAATGCCAGTCACATTCACTAGAGGAGGAGGTGGCTCCAGGTTCCTGCAGAAATGATCCCAGGGACAAG
CCAAGGCTCAGGACTTCAACACAGCAGGAACAGAGCTGTAGCTGGAGTTCCACAGAGAGATCTGGTGCAG
ACTCCCCGCCTGGTCCAGGGCTTAATAGACCAACAAGTTGTAACAACTCAGCTGAGAACAGAGATGGCAG
AGGTGACGGTTTAGACTGGTTGGACCCCACATCAAGTCCTGAGATTGCACCAGAAAGCACTATTGTGTCT
CCCAATGTGAAAACAACTGAGAAAAATGTGAATTTGAAAATCTCCAACAATAAATCTCAGGGCAAGGAGA
AGCATGGGCCACAGCAAAGAAGCAAATTATTAGAAGCTGGACATCTTCCATCATCAGGAGCCATGAATGC
CCCCTTACGGTCTCACGGTGTTAAGCGTACAAAGGCAAGTCAGGCAGTGGTTGTATCTGAAGCAGGACGG
GGTGATGAAGAAGACTCTGTGCCCCGAAGACTCCCCAAGCTGCTGAAGGAGGGGTCACAGAATGTGTGCA
GAAGCACAACCAGAGTGCGACCACTGCCACCCACTGTCCCTCTGTCCCTGTCTATCCCTTCACCTGGGTC
AGGAAAAAGATCAGGAACACCCAAAAGAAAGAGACGGAAATCTGCTCAGGTGGAAGAGCCTGAACCTGAA
GGTATGGAGACTCCAACAGTAAAGCTGGCCAAATTCACATTTAAACAGAAGACAAAACTGACCCACTCCC
CCGAAGGCCAAGGCCCCATACCTCCCAGTGCTAGTGAAATAGCAGTTGACAGTTCTAAAATTCCCCAACA
AAGAACAAGAAGAGAAGCAGCTGTGCCTGTGGTAGCGCCAGGAAAGTCGACATCCACCTCAGGAGACAGA
TGCTCTGACCAGCTGCACGGAAAGACAAAGGAGCTCTCAAGGCAGCCACCAGACAGTAATCCACCAAGAG
AGGAGAGGGAACAGGGCCCCAAAAGAAGGGTTATTCAGCCCAAACCGGAGCTTGGGAACCAGGCTGGGCA
TTCGCATCTAGCCTGTGAGAAAGACAGAAAGGAAGGGGTTTCCTGCGGTAATAAAAGCAGCAAGGTTCAT
GCTGGCACCATAGCCAGACTGGCAAGCTTCTCCTTCACTTCCCCATCAGAATCCAAATCGGAATCCCTCC
CTCCTGAAAGGAAGGACAGCAGGGACAGCAGGGACAGCAGGGACAGCAGGGACAGGTGCCACAGCCCTCC
TGCGACCACAGCTCCAGTGCTCGGCCAGCAAAGGCAGACTTTCCAGCTGCAGCAGCCCACAGAGAGAGCG
AATCTTTCCACCCTCTCCCTCTTCACTCTGTCGGAACTAGATGACGAAGCATTAGATTTTGACTGGGAGG
AAGAGATGAGGAAAAAGCCATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027830
Insert Size 3873 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027830.2, NP_082106.2
RefSeq Size 3873 bp
RefSeq ORF 3873 bp
Locus ID 71567
UniProt ID Q2KHI9
Gene Summary Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855, PubMed:22771120). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex at double-stranded DNA breaks to generate ssDNA by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (By similarity). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:22771120, PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:22771120, PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (By similarity). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:21987787). Probably by regulating HR, plays a key role during gametogenesis (PubMed:21987787, PubMed:22771120).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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