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Crb2 (NM_001163566) Mouse Untagged Clone
CAT＃: MC224155
Crb2 (untagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2), (10ug)
CN¥ 16,530.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5930402A21; BC043114 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224155 representing NM_001163566
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGGTGGGGCCTAGGATTTGGGGCCCTAGACGAGATATTTACCCCCTGTTGCTGCTACTGCTAC TGCTGCTGCTGCTGCTGCTGCCTTGGGTCCCGGCTGGGCTGGTTCCTCCAGAGACTCCAAGTGTCTGTGC CTCAGACCCATGTGCTCCAGGGACCAAGTGCCAGGCTACAGAAAGTGGTGGCTATACCTGTGAGCCCTCA GAGCTTGGAGGCTGTGCTACTCAGCCATGCCACCACGGGGCACTGTGTGTGCCCCAGGGCCCAGATCCTA ACAGCTTCCGTTGCTACTGTGTGCCTGGATTCCAGGGACCCCACTGTGAACTGGACATCGATGAGTGTGC GTCCCGGCCTTGCCAGCATGGGGGCACCTGCCAAAATCTGGCAGATCACTACGAGTGCCACTGTCCCCTG GGATATGCAGGCGTGACCTGTGAGGCCGAGGTGGACGAGTGCTCGTCAGCGCCCTGCCTGCACGGAGGCT CGTGCCTGGACGGTGTGGGTTCCTACCGCTGCGTGTGCGCGCCGGGATACGCCGGTGCGAACTGTCAGCT GGACGTGGACGAGTGCCAGAGCCAGCCGTGCGCGCACGGTGGCGTGTGTCACGACCTGGTCAACGGTTTC CGGTGCGACTGCGCGGACACGGGTTACGAAGGCGCGCGCTGCGAGCAGGAGGTGCTGGAGTGCGCCTCTG CGCCCTGCGCGCACAACGCGTCCTGCCTCGACGGCTTCCGGAGCTTCCGCTGCCTCTGCTGGCCAGGCTT CAGTGGAGAGCGGTGCGAAGTGGATGAGGACGAATGTGCATCGGGCCCCTGTCAAAATGGGGGCCAGTGC TTGCAGCGCTCTGACCCGACCTTGTACGGGGGTGTTCAGGCCATCTTCCCGGGAGCCTTCAGCTTCAGCC ACGCCGCTGGCTTCCTTTGCAGCTGTCCTCTGGGCTTTGCTGGGAATGACTGCAGCATGGATGTGGATGA GTGTGCCTCAGGGCCGTGCCTCAATGGAGGTAGCTGCCAGGACCTGCCCAATGGTTTCCAGTGCTACTGC CAGGATGGATACACAGGCCTGACATGTCAGGAAGATATGGATGAATGCCAGTCGGAACCATGCCTGCATG GTGGAACCTGCAGCGACACTGTAGCAGGCTACATCTGCCAGTGCCCTGAGGCCTGGGGTGGACATGACTG TTCTGTGCAGCTCACAGGCTGCCAGGGCCATACCTGCCCACTGGCTGCCACCTGCATCCCCACCTTCAAG TCTGGCCTCCACGGTTACTTCTGCCGCTGCCCACCGGGGACCTATGGGCCTTTCTGTGGCCAGAATACGA CCTTCTCGGTTGTGTCTGGGAGTTCTGTGTGGGGATTGGTGCCAGCTGCTGCCTCCCTGGGTCTGGCACT GAGATTTCGTACCACCCTGCTTGCCGGAACCCTGGCTACTCTCAAAGACACTCGGGACAGCTTGGAGCTG GTTCTGGTGGGGGCCGTGCTCCAAGCCACACTCTCGAGACATGGCACTGCGGTGCTCATCCTTACACTGC CTGACCTAGCCTTAAATGATGGGCATTGGCACCAGGTGGAAGTGACGCTCCACCTGGGAACCCTGGAGCT GCGGCTCTGGCACGAGGGCTGCCCTGGCCAGCTCTGTGTGGCCTCTGGTCCGGTGGCTACAGGCCCTACA GCCTCGGTGGCCTCTGGGCCTCCGGGATCCTACTCCATCTATCTGGGCGGCGGGGTCTTTGCCGGCTGCT TCCAGGATGTGCGTGTGGAAGGGCACCTTCTGCTGCCTGAGGAGCTCAAGGGAACTGTGCTTCTGGGTTG TGAGCGCAGGGAACCTTGCCAGCCTCTGCCTTGTGCCCATGGAGGGGCCTGCGTGGACCTGTGGACTCAC TTCCGCTGCGACTGCCCGAGACCTTACCGTGGAGCCACATGCACTGATGAGGTTCCTGCTGCCACTTTTG GCTTGGGTGGAGCCACGAGCTCAGCCTCTTTTCTGCTCCACCAACTAGGCCCAAACCTCACCGTGTCATT TTTCCTCCGCACCCGCGAACCTGCCGGCCTGTTGCTCCAGTTTGCCAATGATTCAGTTGCGAGCCTGACT GTGTTCCTGAGTGAGGGCCAGATCCGGGCTGAGGGGCTGGGTCACCCTGCTGTGGTCCTCCCTGGGCGCT GGGATGATGGACTCCCCCACTTGGTGATGCTCAGCTTTGGGCCTGACCAGCTGCAGGACCTGGGCCAGCG GCTGTATGTGGGTGGGAGGTTCTACCCTGATGACACCCAGCTCTGGGGTGGGCCCTTCCGAGGCTGTCTC CAGGACCTACAACTCAACAGCATCCACCTCCCCTTCTTCTCTTCCCCGATGGAGAACTCAAGTTGGCCCA GTGAACTGGAAGCTGGCCAGTCCTCCAACCTCACCCAGGGTTGTGTCTCTGAGGACACGTGCAATCCCAA TCCCTGTTTCAATGGTGGCACGTGCCACGTCACCTGGAATGACTTCTACTGCACCTGCTCCGAGAACTTC ACGGGGCCCACCTGTGCCCAGCAGCGATGGTGCCCCAGGCAGCCATGCCTGCCTCCTGCCACCTGTGAGG AGGTTCCAGATGGCTTTGTGTGTGTGGCCGAGGCCACGTTCCGCGAGGGCCCTCCTGCTGTGTTCACAGG CCACAACGTGTCCTCATCGCTCAGCGGGCTCACCCTGGCCTTCCGCACGCGCGACTCCGAGGCTGGGCTA CTGCGCGCCGTCTCCGCCGCAGGTGCCCACTCCAATATCTGGTTGGCGGTGCGCAACGGCTCGCTGGCAG GAGATGTGGCGGGTTCGGTGCTGCCCGCGCCCGGGCCGCGCGTGGCCGACGGCGCCTGGCATCGCGTGCG CCTAGCCCGGGAGTTCCCACAGGCCGCTGCCTCGCGCTGGCTGCTGTGGCTGGACGGCGCGGCGACACCC GTGGCCTTGCACGGCTTGGGCGGCGACCTGGGCTTTCTGCAGGGTCCGGGTGCAGTGCCTCTGCTACTGG CTGAGAACTTCACGGGCTGCCTGGGCCGCGTGGCACTTGGCGACTTCCCGTTACCCTTGGCGCCACCGCG GTCTGGCACGGTGTCTGGGGCGCGCGAGCACTTCGTGGCCTGGCCCGGGTCTCCGGCCGTGAGTCTCGGC TGCCGTGGCGGACCCGTATGTTCACCCTCGCCCTGCCTGCACGGTGGCGCCTGCCGCGACCTCTTCGACG CCTTTGCTTGCTCCTGCGGCCCCGCCTGGGAAGGACCCCGCTGCGAGATCCGCGCTGACCCATGTCGCTC CACTCCCTGTGTCCGGGGCCAATGCCATGCGCGCCCCGACGGCCGCTTCGAGTGTCGCTGCCCTCCGGGC TTCTCCGGCCCGCGCTGCAGGTTGCCTGTCCTGCCTCAGGGATGCAACCTCAACTCCACATGCAAGGATG GCGCCCCTTGTGAAGGCGGCCCCTTAGGCACCAACTGCAGTTGCCAGGAAGGTCTTGCTGGCCTCAGATG TCAGAGTCTCGACAAACCCTGTGAAGCCAGCCCTTGCTTGAATGGGGGCACTTGCCGGGTGGCCAGTGGC ATATTTGAATGTACTTGCAGTGCAGGATTCTCTGGCCAGTTCTGTGAAGTGGTGAAAACCCTACCTCTGC CTCTGCCGTTCCCACTGCTGGAGGTAGCAGTGCCTGCAGCCTGTGCCTGCCTCCTCCTTCTCCTCCTGGG CCTCCTCTCAGGAATCCTGGCTGCCAGAAAGCGCCGCCAGTCTGAGGGCACTTACAGCCCAAGTCAGCAG GAGGTGGCTGGGGCTCGTCTGGAGATGGACAGTGTCCTCAAGGTGCCACCAGAGGAGAGACTTATTTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001163566 |
| Insert Size | 3849 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001163566.1, NP_001157038.1 |
| RefSeq Size | 6393 bp |
| RefSeq ORF | 3849 bp |
| Locus ID | 241324 |
| UniProt ID | Q80YA8 |
| Gene Summary | Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo (PubMed:26496195, PubMed:27870829). Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer (PubMed:27870829). The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress: cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB (PubMed:27870829). Also required for maintenance of the apical polarity complex during development of the cortex.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222655 | Crb2 (tGFP-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2), (10ug) |
CN¥ 14,992.00 |
|
| MR222655 | Crb2 (Myc-DDK-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) |
CN¥ 13,392.00 |
|
| MR222655L3 | Lenti ORF clone of Crb2 (Myc-DDK-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) |
CN¥ 14,730.00 |
|
| MR222655L4 | Lenti ORF clone of Crb2 (mGFP-tagged) - Mouse crumbs homolog 2 (Drosophila) (Crb2) |
CN¥ 14,730.00 |
