Nid1 (NM_010917) Mouse Untagged Clone

CAT#: MC224059

Nid1 (untagged) - Mouse nidogen 1 (Nid1), (10ug)



  "NM_010917" in other vectors (4)

CNY 16,060.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A630025O17; entactin; entactin-1; Nid; nidogen-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224059 representing NM_010917
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGACGCGAGCGGCTGTAGTTGGGCGATGTGGACGTGGGCGCTGTTGCAGCTGCTGCTACTAGTGG
GGCCCGGAGGCTGCCTGAATCGCCAGGAGCTCTTCCCCTTCGGCCCTGGGCAGGGGGACCTAGAGCTGGA
GGCCGGGGACGACGTGGTATCCCCCTCCCTGGAACTGATCGGAGAACTGAGCTTCTATGATCGTACGGAC
ATCACGTCGGTCTATGTCACCACAAATGGTATCATCGCCATGAGCGAACCCCCAGCCACAGAATACCATC
CCGGAACCTTCCCACCCAGCTTCGGCTCAGTAGCGCCTTTCCTGGCTGACTTGGACACAACCGATGGCCT
GGGGAATGTGTATTATCGAGAAGACTTATCCCCGTTCATTATTCAGATGGCAGCAGAGTATGTCCAGAGA
GGCTTCCCGGAGGTCTCTTTCCAGCCCACTAGTGTGGTGGTTGTCACTTGGGAATCTGTGGCCCCTTATG
GAGGGCCCAGCAGCAGCCCTGCCGAGGAAGGCAAGAGAAACACGTTCCAGGCTGTTCTGGCCTCCTCAAA
TTCCAGCTCCTATGCCATTTTCCTTTATCCTGAAGATGGTCTACAGTTCTTTACAACATTCTCGAAGAAG
GATGAAAGCCAAGTACCTGCTGTGGTTGGTTTCAGCAAAGGTCTAGTAGGATTTCTATGGAAGAGCAACG
GAGCCTATAACATATTTGCCAATGACAGAGAATCAATTGAAAATTTGGCTAAGAGCAGCAACGCTGGGCA
CCAGGGTGTGTGGGTGTTTGAGATTGGAAGTCCTGCTACCGCCAAGGGCGTGGTGTCTGCAGATGTGAAC
CTGGACCTGGACGACGATGGAGCAGACTATGAAGATGAAGATTATGACCTAGTAACCTCTCACCTTGGCC
TGGAGGATGTAGCCACTCCCTCCCCCTCCCACAGTCCAAGAAGGGGATACCCTGACCCACACAATGTACC
TAGGATCCTCTCTCCTGGCTATGAGGCTACAGAGAGACCCCGTGGAGTCCCCACTGAGAGGACCAGATCT
TTCCAGCTGCCAGCGGAGAGGTTCCCTCAGCATCACCCCCAGGTCATTGATGTGGATGAAGTAGAGGAAA
CAGGAGTTGTATTCAGCTACAACACAGGTTCCCAGCAGACTTGTGCCAACAATAGACACCAGTGCTCCGT
GCATGCAGAGTGCAGAGACTATGCTACTGGCTTCTGCTGCAGGTGTGTGGCCAACTACACAGGCAATGGC
AGACAGTGCGTGGCAGAAGGCTCTCCACAACGGGTCAATGGCAAGGTGAAGGGAAGGATCTTCGTGGGGA
GCAGCCAGGTCCCCGTGGTGTTTGAGAACACTGACCTGCACTCCTATGTGGTGATGAACCACGGGCGCTC
TTACACAGCCATCAGCACCATCCCTGAAACCGTCGGCTACTCTCTGCTCCCCCTGGCACCCATTGGAGGC
ATCATCGGATGGATGTTTGCAGTGGAGCAGGATGGGTTCAAGAATGGGTTTAGCATCACTGGGGGCGAGT
TTACCCGGCAAGCTGAGGTGACCTTCCTGGGGCACCCAGGCAAGCTGGTCCTGAAGCAGCAGTTCAGCGG
TATTGATGAACATGGACACCTGACCATCAGCACGGAGCTGGAGGGCCGCGTGCCGCAGATCCCCTATGGA
GCCTCGGTGCACATTGAGCCCTACACCGAACTGTACCACTACTCCAGCTCAGTGATCACTTCCTCCTCCA
CCCGGGAGTACACGGTGATGGAGCCTGATCAGGACGGCGCTGCACCCTCACACACCCATATTTACCAGTG
GCGTCAGACCATCACCTTCCAGGAGTGTGCCCACGATGACGCCAGGCCAGCCCTGCCCAGCACCCAGCAG
CTCTCTGTGGACAGCGTGTTTGTCCTGTACAACAAGGAGGAGAGGATCTTGCGCTATGCCCTCAGCAACT
CCATCGGGCCTGTGAGGGATGGCTCCCCTGATGCCCTTCAGAATCCATGCTACATTGGCACCCATGGGTG
TGACAGCAATGCTGCCTGTCGCCCTGGCCCTGGAACACAGTTCACCTGCGAATGCTCCATCGGCTTCCGA
GGAGACGGGCAGACTTGCTATGATATTGATGAGTGTTCAGAGCAGCCTTCCCGCTGTGGGAACCATGCGG
TCTGCAACAACCTCCCAGGAACCTTCCGCTGCGAGTGTGTAGAGGGCTACCACTTCTCAGACAGGGGAAC
ATGTGTGGCTGCCGAGGACCAACGTCCCATCAACTACTGTGAAACTGGTCTCCACAACTGTGATATCCCC
CAGCGAGCCCAGTGCATCTATATGGGTGGTTCCTCCTACACCTGCTCCTGTCTGCCTGGCTTCTCTGGGG
ATGGCAGAGCCTGCCGAGACGTGGATGAATGCCAGCACAGCCGATGTCACCCCGATGCCTTCTGCTACAA
CACACCAGGCTCTTTCACATGTCAGTGCAAGCCTGGCTATCAGGGGGATGGCTTCCGATGCATGCCCGGA
GAGGTGAGCAAAACCCGGTGTCAACTGGAACGAGAGCACATCCTTGGAGCAGCCGGCGGGGCAGATGCAC
AGCGGCCCACCCTGCAGGGGATGTTTGTACCTCAGTGTGATGAATATGGACACTATGTACCCACCCAGTG
TCACCACAGCACTGGCTACTGCTGGTGTGTGGACCGAGATGGTCGGGAGCTGGAGGGTAGCCGTACCCCA
CCTGGGATGAGGCCCCCGTGTCTGAGTACAGTGGCTCCTCCTATTCACCAGGGACCAGTAGTACCTACAG
CTGTCATCCCCCTGCCTCCAGGGACACACTTACTCTTTGCTCAGACTGGAAAGATTGAACGCCTGCCCCT
GGAGAGAAACACCATGAAGAAGACAGAAGCCAAGGCCTTTCTCCATATCCCTGCAAAAGTCATCATTGGA
CTGGCCTTTGACTGCGTGGACAAGGTGGTTTACTGGACAGACATCAGCGAGCCTTCCATTGGGAGAGCCA
GCCTCCACGGTGGAGAGCCAACCACCATCATTCGACAAGATCTTGGAAGCCCTGAAGGCATTGCCCTTGA
CCATCTTGGTCGAACCATCTTCTGGACGGACTCTCAGTTGGATCGAATAGAAGTTGCAAAGATGGATGGC
ACCCAGCGCCGAGTGCTGTTTGACACGGGTTTGGTGAATCCCAGAGGCATTGTGACAGACCCCGTAAGAG
GGAACCTTTATTGGACAGATTGGAACAGAGACAATCCCAAAATTGAGACTTCTCACATGGATGGCACCAA
CCGGAGGATTCTCGCACAGGACAACCTGGGCTTGCCCAATGGTCTGACCTTTGATGCATTCTCATCTCAG
CTTTGCTGGGTGGATGCAGGCACCCATAGGGCAGAATGCCTGAACCCAGCTCAGCCCGGCAGACGCAAAG
TTCTCGAAGGGCTCCAGTATCCTTTCGCTGTGACTAGCTATGGGAAGAATTTGTACTACACAGACTGGAA
GACGAATTCAGTGATTGCCATGGACCTTGCTATATCCAAAGAGATGGATACCTTCCACCCACACAAGCAG
ACCCGGCTATATGGCATCACCATCGCCCTGTCCCAGTGTCCCCAAGGCCACAATTACTGCTCAGTGAATA
ATGGTGGATGTACCCACCTCTGCTTGCCCACTCCAGGGAGCAGGACCTGCCGATGTCCTGACAACACCCT
GGGAGTTGACTGCATTGAACGGAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010917
Insert Size 3738 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010917.2, NP_035047.2
RefSeq Size 6046 bp
RefSeq ORF 3738 bp
Locus ID 18073
UniProt ID P10493
Gene Summary Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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