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Smc1a (NM_019710) Mouse Untagged Clone
CAT＃: MC224011
Smc1a (untagged) - Mouse structural maintenance of chromosomes 1A (Smc1a), (10ug)
CNY 15,770.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5830426I24Rik; mKIAA0178; Sb1.8; SMC-1A; Smc1; Smc1alpha; Smc1l1; Smcb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224011 representing NM_019710
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTCCTGAAACTGATTGAGATCGAGAACTTTAAATCGTACAAGGGTCGACAGATTATCGGACCAT TTCAGAGGTTTACCGCCATCATTGGACCCAATGGCTCTGGTAAGTCAAATCTCATGGATGCCATCAGCTT TGTGCTTGGTGAAAAGACCAGCAACCTGAGGGTAAAGACCCTGCGGGACCTGATACATGGAGCACCTGTG GGCAAGCCGGCTGCCAACCGAGCCTTTGTCAGCATGGTCTACTCGGAGGAGGGTGCTGAGGACCGTACCT TTGCCCGAGTCATTGTAGGGGGCTCTTCTGAGTACAAGATCAACAACAAAGTTGTCCAGCTACATGAATA CAGTGAAGAGTTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTCTTCCAGGGTGCAGTG GAGTCTATTGCCATGAAGAACCCCAAGGAGAGGACAGCTCTGTTTGAAGAAATTAGTCGCTCTGGGGAAC TTGCACAGGAGTATGACAAGCGAAAGAAAGAAATGGTGAAGGCTGAAGAAGACACACAGTTTAATTATCA TCGCAAGAAAAATATTGCAGCTGAACGAAAGGAGGCCAAACAAGAAAAGGAAGAGGCGGACCGCTATCAG CGCCTGAAGGATGAGGTAGTACGAGCACAGGTACAGTTGCAGCTCTTTAAACTCTACCACAACGAAGTGG AGATTGAGAAGCTCAACAAGGAACTGGCCTCGAAGAACAAGGAAATTGAGAAGGACAAGAAGCGGATGGA CAAGGTAGAGGATGAGCTGAAGGAGAAGAAGAAAGAGCTGGGAAAAATGATGCGGGAACAGCAGCAGATT GAGAAGGAGATCAAGGAAAAGGACTCGGAATTGAATCAGAAGAGGCCTCAGTACATCAAAGCAAAAGAGA ATACATCCCACAAAATCAAGAAGCTCGAAGCAGCCAAGAAGTCTCTTCAAAATGCTCAGAAACATTATAA GAAACGTAAAGGTGACATGGATGAGCTGGAGAAGGAGATGCTGTCAGTGGAGAAGGCCCGACAGGAGTTT GAAGAACGGATGGAGGAAGAGAGTCAGAGTCAGGGCAGAGACTTGACCCTGGAGGAGAACCAGGTAAAGA AATACCACCGATTGAAAGAAGAAGCCAGCAAGAGAGCAGCTACTTTGGCTCAGGAGCTGGAGAAGTTCAA TCGAGACCAGAAAGCTGACCAGGACCGTCTAGATCTGGAAGAGCGGAAGAAAGTAGAAACAGAGGCCAAG ATCAAGCAAAAACTGCGGGAGATTGAAGAGAATCAGAAACGGATTGAGAAGCTAGAAGAATATATTACCA CTAGCAAGCAGTCTCTAGAGGAGCAGAAGAAGCTAGAGGGAGAACTGACGGAAGAAGTAGAGATGGCCAA GCGTCGTATTGATGAGATCAATAAGGAGCTGAACCAGGTGATGGAGCAATTAGGGGATGCACGAATTGAC CGACAGGAGAGTAGCCGCCAGCAGCGAAAGGCAGAAATAATGGAAAGCATCAAGCGTCTCTACCCTGGCT CTGTGTATGGCCGCCTCATTGACCTCTGCCAGCCTACACAAAAGAAGTATCAGATTGCTGTGACCAAGGT TTTAGGGAAGAACATGGATGCTATTATTGTAGACTCAGAGAAGACAGGTCGGGACTGTATTCAGTATATC AAGGAGCAACGTGGAGAGCCTGAGACCTTCTTGCCTCTTGACTACCTGGAGGTGAAACCTACTGATGAGA AACTCCGGGAGCTGAAGGGGGCCAAGCTAGTGATTGATGTAATTCGTTATGAACCACCTCACATTAAAAA AGCTCTGCAGTATGCTTGTGGCAATGCCCTTGTTTGTGATAATGTGGAGGATGCTCGACGCATTGCCTTT GGAGGTCACCAACGCCACAAGACAGTGGCGTTGGATGGCACCCTGTTCCAGAAGTCAGGAGTTATCTCTG GTGGAGCCAGTGATCTCAAGGCAAAAGCACGGCGCTGGGATGAGAAAGCAGTAGACAAACTGAAAGAAAA AAAAGAACGTTTGACAGAGGAGCTGAAAGAGCAGATGAAGGCAAAACGGAAAGAGGCAGAACTGCGTCAA GTGCAGTCTCAGGCCCACGGGCTTCAAATGCGGCTGAAGTACTCACAGAGTGACCTAGAACAGACTAAGA CAAGGCATCTTGCCCTGAATCTCCAGGAAAAGTCTAAGCTGGAGAGTGAACTAGCCAACTTCGGGCCTCG CATTAATGATATCAAGAGAATCATACAGAGCCGAGAGAGGGAAATGAAAGACTTGAAGGAGAAGATGAAC CAGGTGGAAGATGAGGTGTTTGAAGAGTTTTGTCGGGAAATTGGTGTGCGTAACATCCGAGAATTTGAGG AAGAAAAGGTGAAGCGGCAGAATGAAATTGCCAAGAAACGCTTGGAATTTGAGAATCAGAAGACTCGCTT GGGCATCCAATTGGATTTTGAAAAGAATCAGTTGAAGGAGGACCAGGATAAAGTACACATGTGGGAACAG ACGGTGAAAAAGGATGAAAATGAGATAGAAAAGCTTAAGAAGGAGGAACAAAGACACATGAAGATAATAG ATGAAACCATGGCTCAGCTTCAAGACCTTAAGAATCAGCACCTGGCCAAAAAGTCAGAAGTAAACGACAA GAATCATGAGATGGAAGAGATTCGCAAGAAGCTTGGGGGTGCCAACAAGGAAATGACTCATTTACAGAAG GAAGTGACAGCCATTGAGACCAAACTTGAACAGAAGCGCAGTGACCGCCACAACCTGCTACAGGCCTGTA AGATGCAGGACATCAAACTGCCACTGTCAAAGGGCACCATGGATGATATTAGTCAGGAAGAAGGTAGCTC CCAGGGAGAAGAGTCAGTGAGTGGTTCCCAGAGAACATCCAGTATCTATGCTCGAGAGGCCCTCATTGAG ATTGACTACGGTGACCTGTGTGAAGATCTGAAGGATGCACAGGCGGAGGAAGAAATCAAGCAAGAAATGA ACACACTCCAGCAAAAGCTGAATGAGCAGCAGAGTGTGCTTCAGCGGATTGCTGCCCCTAACATGAAGGC CATGGAAAAGCTGGAGAGTGTCCGAGACAAGTTCCAGGAGACCTCAGATGAGTTTGAAGCAGCACGAAAG CGAGCAAAGAAGGCCAAGCAGGCATTTGAACAGATAAAGAAGGAGCGCTTCGACCGATTCAATGCTTGCT TTGAATCTGTGGCTACCAACATTGATGAGATCTATAAGGCTCTGTCCCGTAACAGCAGTGCCCAGGCATT CTTGGGCCCTGAGAATCCTGAAGAGCCCTATTTGGATGGCATCAACTACAACTGTGTAGCTCCTGGCAAA CGTTTCCGGCCTATGGACAATTTGTCAGGCGGGGAGAAGACAGTTGCAGCTCTGGCCTTGCTTTTTGCCA TCCATAGCTACAAGCCAGCCCCCTTCTTCGTCCTGGATGAGATTGACGCTGCCTTGGATAACACCAACAT TGGCAAGGTGGCAAATTACATCAAGGAACAGTCAACTTGCAACTTCCAGGCCATTGTTATCTCTCTCAAG GAGGAATTCTACACCAAGGCTGAGAGCCTCATTGGAGTCTATCCTGAGCAAGGGGACTGTGTGATCAGCA AAGTCCTGACTTTCGACCTCACCAAGTACCCAGATGCCAACCCCAACCCCAATGAACAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019710 |
| Insert Size | 3702 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019710.2, NP_062684.2 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 3702 bp |
| Locus ID | 24061 |
| UniProt ID | Q9CU62 |
| Gene Summary | Involved in chromosome cohesion during cell cycle and in DNA repair. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint (By similarity). Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221566 | Smc1a (tGFP-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a), (10ug) |
CNY 13,490.00 |
|
| MR221566 | Smc1a (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a) |
CNY 12,260.00 |
|
| MR221566L3 | Lenti ORF clone of Smc1a (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a) |
CNY 14,160.00 |
|
| MR221566L4 | Lenti ORF clone of Smc1a (mGFP-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a) |
CNY 14,160.00 |
