Irs4 (NM_010572) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	IRS-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223965 representing NM_010572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGTTGCTCCTTCTCTGGCCACCAAGCGCTTAGGAGACTGAGAGCCTCAGCAGCAGCAGCAGCCT CCGCAGCTCTAGCAGCAGTGGCGACCACCCCGCTCCTTTCCTCGGGAACCCGGACCGCACTCATTGGGAC CGGGTCGTCCTGTCCGGGAGCCATGTGGCTCTCCACAGCCACTGGCTCCCGGTCAGACTCGGAATCCGAA GAGGAGGACCTCCCCGTCGGGGATGAAGTCTGCAAACGCGGCTACCTGAGGAAGCAGAAGCATGGGCATA GGCGTTACTTCGTGCTCAAACTCGAGACCGCAGACGCTCCAGCTCGACTCGAATACTACCGAAATGCCAG GAAGTTCCGGCACAGTGTCCGCGCCGCGGCGGCTGCAGCAGAGGCGGCCGCCTCCGGTGCTGCGGTCCCC GCGCTCATCCCACCACGGCGCGTGATCATCCTGTACCAATGCTTCTCCGTGAGCCAGCGCGCCGATGCCA GGTACCGACACCTCATTGCCCTTTTTACCCAGGACGAATACTTCGCGATGGTGGCCGAGAACGAGTCGGA ACAAGAAAGCTGGTACTTGCTCCTCAGCCGCCTCATCCTCGAGAGCAAGCGCCGCCGCTGCGGCACGCTA GGCGCACTGCCGGACGGAGAGCCGGCAGCCCTGGCGGCCGCAGCGGCGGCGGAGCCACCCTTCTACAAAG ATGTGTGGCAGGTAGTAGTGAAACCCAGGGGGCTGGGGCACAGAAAAGAGCTGAGCGGCGTGTTCCGGCT GTGTCTTACCGACGAAGAGGTAGTGTTTGTAAGGCTCAATACCGAAGTGGCCAGTGTGGTCGTCCAGCTG CTGAGCATTCGTCGCTGTGGGCACTCGGAGCAGTACTTCTTCTTGGAAGTCGGCAGATCCACTGTCATAG GTCCGGGGGAGCTGTGGATGCAAGTGGATGACTCCGTGGTGGCCCAAAATATGCATGAGCTGTTTCTGGA GAAGATGAGAGCCTTGTGCGCAGATGAATACAGAGCCCGATGCCGCAGCTACAGCATCAGTATTGGCGCC CACCTGTTAACCCTGCTGTCCACTAGGAGGCACCTGGGCTTGCTCCCGCTGGAGCCTGGTGGCTGGCTCA GAAGATACGGCTTGGAGCAGTTTTGCCGCCTCAGAGCTATCCGTGAGAGGGAAGAGATGCTATTCACCAG GCGCTTCATCTCACCCAGAGAGCCTCCGCCTCCCTTTAGGCGAGGAAGAGGGCACCTGCCCAGAGCCCGC AGGTCCAGGAGAGCCGCGTCAGTACCACCCAGCCTTTTCCGACGCTCAGCACCTAGCCCGGGAAGGATCC CACAGCCTGAAGATGTCCCCAACGACAGAGCCCGTGAAGCCTCCGGTTCCAGCTCTGGCAACACTGAAGA AAAAGACAAGGAGGGTGAGGAAGGAAACCGAGGTGATTGCATACCTATGAACAACTGGGGCTCAGGAAAT GGCCGGGGCTCAGGAGGTGGACGGGGCTCAAGTGGCCAAGGTTCCAGTAGCCAGGGCTCAGGTGGGAGAC AGGGATCTGGAGGTGGCCAGGGCTCAGGAGGCCAGGGTGCCGGAGGAAACCAGTGTTCAGGAAATGGCCA GGGCACAGCAGGTGGCCATGGCTCAGGTGGAGGTGGCCATGGCTCAGGCGGTGGCCAGAGACCTGGAGAT GGTCATGGCTCAGGTGGTGGCAAGAACTCAGGAAGTGGGAAAAATTCTGACGATGGTGACCGTGGAAAAT CTGTGAAGAAAAGATCCTACTTTGGTAAATTCACTCAAAGCAAGCAGCAGCAAACACTGCCTCCACCCCC ACCGCCACCGCCAGCAGCTGGAGCAACTGGTGGCAAAGGGAAGTCTGGAGGAAGATTCCGACTTTATTTT TGCGCAGACAGAGGCACAAAAGAACGCAAAGAAGCCAAAGAGGTAAGAGATATGGAGACCTCAGGAGGTG CTACCCGGGGGCCTTACAGAGCCAGAGCTTTCGATGAAGATGAGGATGACCCGTATGTGCCAATGAGACC AGGGGTGGCTGCCCCTCTTGCGTGTTCCAGTGATTATATGCCAATGGCTCCTCAAAATTCCTCTGCTTCA ACAAAACGCCACTCTAGGTCACCCTTTGAAGACTCCAGAGGGTACATGATGATGTTTCCCAGAGTGAGCC CCCCACCACCTGTGCCAAGTGCTCCGAAAGCACCGGACACCAATAAAGGGGATGACTCAAAGGACAATGA CAGTGACAGTGATTACATGTTTATGGCTCCCGGAGCAGGTGCGATTCCTAAAAATCCTCCCAATGCGCAG GGAGGCTCTTCCTCCAAAAGCTGGAGCTCGTATTTCTCTCTGCCAAGTCCTTTTCAGAGTTCACCTTTGG GACAGAGTGACCACAGTGAGTATGTTCCTATGTTACCTGGAAAATTCCTGGGGAGCGGTCTACACAAAGA AGCCTCCTTTAGCCAGGGCACCAAAAATGTGTCTTCAAAGCCTTCAACTGAGGCGTCATTCTCAAAACCC GAAGATAAAGGGTCATCTGCAAAGCCTTCAGATGATGTGCCCCCAATGAACAAGGCTAAGGAACCTAACC ACCTTTCTTTTATTGCAAAAGGAACTCAAGTAAAGCCCAAACCGCTAAACCCCACACAGGAGCGGAGAGA AGCTGCTGGCTCCCGTGACTATATCAACATTGACTTTATTAAGAGAGAGCGCCTTGTGCTACCTTCCTCT GCTCAAGGACTGCCAGACATGCGGGGCGTAGTTACTGACCCCGCACCCACAGCTTTTTCTGGCTACCTGA ATGTTGAATTCGGGGTGCCCTTCCCAAATCCAACAATCCGCCTCTCAGATCTTTTAAGAGTTCTACCAGG TGCCAACTCCATCCCTCTGGCCGGTACTAGGTGGCCCTTCCCAGGCAGTGCTATAGGTAGCATTGTGGAA GCGGGAGAATACATTGAAGTTATTTTCAACCCGGCTATGACACCAGCCATGTCTTTTGCTGACAGTGCCA TTTGCTATGATGCTCAAACAGGTCAAATCTATGTCGTCGACCCATTTTCTGAGTGCTGTATGGACGTTTC TCTCTCTCCTGGTCGATGCTCTGAACCGCCACCTGTAGCTAGGCTGCGGCGGGAGGAAGCGCAGGAGAGA AGACGCCCACAAAGCCGCTCCCAAAGTTTATTTGCCAGCACTCGAGCCGCGGTCTCGGCTTTCCCGACTG ACAGCTTGGACAGAGACTTTCCCGCCGCTTCTGCGGTGATTGCTGCTCCAGCTGAGGCGCCTCTCTTGGC AGTGAGCCGGGCTTTAGCCGTGGTCTCTGCGCTCGCAGCGGCCCCCAGCATAGGCGACGTCTTTGCAGGC TTCCGCGCTGCGGCAGGAGTTGACTCCGCCTCCGCTCGCGGGTTCCAACCTGTTGCGGGTGCCCAGGCTG TAAGAGAATTCCAGGACCTTGCCGCAGGCTGGAATCCTGGAGCCCTTAACCACAGGGCCCGAGGTGAGGA CCTGGCCGCCGGCGCAGCGGCTCCCCCGCCGCCACCTCGCCAGATTTGGGTGCTGAGACCCCAAGAGAGA GCGGATTCTGAGGACGACGACGATGATGACGACGACATTTACGTGAGAATGGACTTTGCCAGACGAGACT ACAGGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010572
Insert Size	3651 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010572.2, NP_034702.2
RefSeq Size	6269 bp
RefSeq ORF	3651 bp
Locus ID	16370
UniProt ID	Q9Z0Y7
Gene Summary	Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.[UniProtKB/Swiss-Prot Function]

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