Ddb1 (NM_015735) Mouse Untagged Clone

CAT#: MC223704

Ddb1 (untagged) - Mouse damage specific DNA binding protein 1 (Ddb1), (10ug)



  "NM_015735" in other vectors (4)

CNY 14,730.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 127kDa; AA408517; p127-Ddb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223704 representing NM_015735
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACTGCAGTGAACGGCTGTGTGACCGGACACTTTA
CTTCAGCGGAAGATCTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACTGCGGA
GGGTCTTCGGCCAGTCAAAGAGGTGGGAATGTACGGGAAGATTGCAGTCATGGAGCTCTTCAGGCCCAAG
GGGGAGAGCAAAGACCTGCTGTTCATCTTGACTGCTAAATACAATGCCTGTATCTTGGAGTATAAGCAGA
GTGGCGAAAGCATTGACATCATTACAAGAGCCCATGGCAATGTCCAGGACCGCATTGGTCGCCCCTCGGA
GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTTCGACTCTACGATGGCCTTTTC
AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAGGAACTGCACG
TCATTGATGTCAAGTTCCTGTATGGTTGTCAAGCACCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG
GCGGCATGTGAAAACCTATGAGGTGTCTCTTCGAGAGAAGGAGTTCAACAAGGGCCCTTGGAAACAGGAG
AATGTAGAAGCCGAAGCTTCAATGGTGATAGCAGTCCCAGAGCCTTTTGGAGGTGCCATCATCATTGGAC
AGGAATCAATCACCTATCATAACGGTGATAAATACCTGGCAATTGCTCCTCCTATCATTAAGCAAAGCAC
TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCGCGGTACCTTCTGGGAGATATGGAGGGACGGCTT
TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACTGTCACCCTCAAGGACCTTCGAGTGGAAC
TCCTCGGAGAGACCTCCATTGCTGAGTGCCTAACTTATCTTGATAATGGTGTTGTATTTGTCGGGTCTCG
CCTAGGTGACTCCCAGCTCGTAAAGCTCAATGTCGACAGCAACGAACAAGGCTCCTATGTAGTGGCCATG
GAAACCTTTACCAATTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC
AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGCATTGGAATCCA
TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCTCTGCGCTCTGACCCCGGTCGGGAG
ACGGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAACGGAGAGGAAGTGG
AAGAGACAGAACTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGTGGCAACGTGGCTCATCAGCA
ACTTATCCAGATCACCTCAGCATCTGTGAGGTTGGTGTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG
AAAGAGCCTCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTTGTTGCCGTGGGAA
GGGCACTATATTACCTTCAGATCCACCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA
AGTGGCATGCTTGGATATCACCCCATTAGGGGACAGCAATGGCCTCTCCCCACTTTGTGCCATTGGACTT
TGGACAGACATCTCAGCTCGTATCCTGAAGCTCCCATCCTTTGAACTACTACACAAGGAGATGCTGGGTG
GAGAGATCATTCCTCGATCCATCCTGATGACCACTTTTGAGAGTAGCCACTACCTCCTTTGTGCCTTGGG
AGATGGGGCTCTTTTCTACTTTGGGCTCAACATCGAGACAGGTTTACTGAGTGACCGTAAAAAGGTGACT
TTGGGCACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCTG
ACCGGCCCACTGTCATCTATAGCAGCAATCACAAGTTGGTCTTCTCCAATGTCAACCTCAAGGAGGTGAA
CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC
ATTGGCACCATCGATGAGATCCAGAAGCTCCATATTCGCACAGTTCCCCTCTATGAGTCTCCCAGGAAGA
TCTGCTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTCCAAGATAGCAGTGG
AGGCACTACTGCTTTGAGGCCCAGTGCCAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAACTT
TTCTCCAGCAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAAGTGGAGGTGCACAACCTCCTCATCA
TTGACCAGCACACCTTTGAAGTGCTTCACGCCCATCAGTTTCTACAAAACGAGTATGCCCTCAGCCTGGT
CTCCTGCAAGTTGGGTAAAGACCCCAACACTTACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG
GCAGAGCCCAAGCAGGGTCGTATTGTGGTCTTCCAGTATTCAGATGGAAAACTACAGACTGTGGCTGAAA
AGGAAGTAAAAGGAGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCCAGCATCAATAGCAC
GGTGCGGCTCTATGAGTGGACCACAGAAAAGGAGCTTCGCACTGAGTGTAATCACTACAACAACATCATG
GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGCTCAGTGCTGCTGCTTG
CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT
GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC
AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG
TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGGCTTCTACCCCTACACAGGGCTC
GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCGCTGTCAGAGAGCTGGTATAACCTC
CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGCGTGGGCAAGATTGAGCACTCCTTCTGGA
GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGATCTGATTGAAAGTTT
CCTAGATATCAGCCGCCCTAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG
AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_015735
Insert Size 3423 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015735.1, NP_056550.1
RefSeq Size 4171 bp
RefSeq ORF 3423 bp
Locus ID 13194
UniProt ID Q3U1J4
Gene Summary Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (PubMed:28790135).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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