Slc12a5 (NM_020333) Mouse Untagged Clone

CAT#: MC223609

Slc12a5 (untagged) - Mouse solute carrier family 12, member 5 (Slc12a5), (10ug)



  "NM_020333" in other vectors (4)

CNY 14,250.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms KCC2; mKIAA1176
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223609 representing NM_020333
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCCGGTGATGGCAACCCCAAAG
AGAGCAGTCCCTTCATCAACAGCACGGACACGGAGAAGGGCAGAGAGTACGATGGCAGGAACATGGCCCT
GTTTGAGGAGGAGATGGACACCAGCCCCATGGTATCCTCCCTGCTCAGTGGGCTGGCCAACTACACCAAC
CTACCCCAGGGAAGTAGAGAGCATGAAGAAGCAGAAAATAATGAGGGTGGAAAAAAGAAGCCGGTGCAGG
CTCCTCGAATGGGCACCTTCATGGGTGTGTACCTGCCGTGCCTGCAGAACATCTTTGGTGTCATCCTCTT
CCTGCGGCTCACGTGGGTGGTGGGCATCGCGGGCATCATGGAGTCCTTCTGTATGGTCTTCATTTGCTGC
TCCTGTACGATGCTCACAGCCATTTCCATGAGTGCAATCGCAACCAATGGTGTTGTGCCTGCTGGTGGCT
CGTACTACATGATTTCCAGGTCTCTGGGCCCGGAGTTTGGGGGCGCCGTGGGCCTCTGCTTCTACCTGGG
CACCACCTTTGCTGGGGCTATGTACATCCTTGGCACGATCGAGATCCTGCTGGCTTATCTCTTCCCAGCT
ATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGAGGCGGCCGCCATGCTGAACAACATGCGGGTGTATG
GCACCTGTGTGCTCACCTGCATGGCCACCGTTGTCTTTGTGGGTGTCAAGTACGTCAACAAGTTTGCCTT
GGTCTTCCTGGGTTGCGTCATCCTGTCCATCCTGGCCATCTATGCAGGGGTCATCAAGTCTGCCTTCGAC
CCACCCAATTTCCCGATCTGCCTCCTGGGGAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTGTGCCA
AGCTGGCTTGGGAAGGAAATGAGACAGTGACCACACGGCTCTGGGGCCTTTTCTGCTCCTCCCGCCTCCT
CAATGCCACCTGTGATGAGTACTTCACCCGAAACAATGTCACAGAGATCCAGGGCATTCCTGGTGCTGCC
AGTGGTCTCATCAAAGAGAACCTGTGGAGTTCTTACCTGACCAAAGGGGTGATTGTCGAGAGGCGTGGGA
TGCCCTCTGTGGGCCTGGCAGACGGTACCCCCGTAGACATGGACCACCCCTATGTCTTCAGTGATATGAC
CTCCTACTTCACCCTGCTCGTTGGTATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGCTCAAACCGA
TCTGGAGACCTGCGGGATGCCCAGAAGTCTATCCCTACTGGAACTATCCTGGCCATTGCTACCACCTCTG
CTGTCTACATCAGCTCTGTTGTTCTGTTTGGAGCCTGCATCGAGGGGGTCGTCTTACGGGACAAGTTTGG
GGAAGCTGTGAATGGCAACTTGGTGGTGGGCACCCTGGCCTGGCCTTCTCCCTGGGTCATCGTCATAGGC
TCTTTCTTCTCTACCTGTGGGGCTGGATTACAGAGCCTCACAGGGGCCCCACGTCTGCTGCAGGCCATCT
CCCGGGATGGCATAGTGCCCTTCCTGCAGGTCTTTGGCCATGGCAAAGCTAATGGAGAGCCAACCTGGGC
GCTGCTGCTGACTGCCTGCATCTGTGAGATCGGCATCCTCATAGCCTCCCTGGATGAGGTCGCCCCTATA
CTTTCCATGTTCTTCCTAATGTGTTACATGTTTGTGAACTTGGCTTGTGCGGTGCAGACGCTGCTGAGGA
CACCCAACTGGAGGCCACGATTTCGCTATTACCACTGGACTCTCTCCTTCCTGGGCATGAGCCTCTGCCT
GGCCCTCATGTTCATTTGCTCCTGGTACTACGCACTGGTGGCCATGCTCATTGCCGGACTCATTTATAAG
TACATCGAGTACCGGGGGGCGGAGAAGGAGTGGGGGGATGGAATCCGAGGCCTGTCTCTCAGTGCAGCAC
GCTATGCTCTCTTGCGCCTGGAGGAAGGACCTCCGCATACGAAGAACTGGAGGCCCCAGCTGCTGGTGCT
GGTGCGTGTGGACCAGGATCAGAACGTGGTGCATCCGCAGCTGCTCTCCCTGACCTCCCAGCTCAAGGCA
GGGAAGGGCCTGACCATTGTGGGCTCCGTCCTTGAGGGCACCTTTCTGGACAACCATCCACAGGCTCAGC
GGGCAGAGGAGTCTATCAGGCGCCTGATGGAGGCTGAGAAGGTGAAGGGCTTCTGCCAGGTAGTGATCTC
CTCCAACCTGCGTGATGGTGTGTCCCACCTGATCCAGTCTGGGGGCCTCGGGGGATTGCAACACAATACC
GTGCTGGTGGGCTGGCCTCGCAACTGGAGGCAGAAGGAGGATCATCAGACATGGAGGAACTTCATCGAAC
TGGTCCGGGAAACTACAGCCGGCCACCTCGCCCTGCTGGTCACCAAGAATGTTTCCATGTTTCCCGGGAA
CCCTGAGCGCTTCTCGGAGGGCAGCATTGACGTGTGGTGGATTGTGCACGACGGGGGCATGCTCATGCTG
CTGCCCTTCCTGCTGCGACACCACAAGGTCTGGAGGAAATGCAAAATGCGGATCTTCACCGTGGCCCAGA
TGGACGATAACAGTATCCAGATGAAGAAGGACCTGACCACGTTTCTGTACCACTTACGCATTACTGCAGA
GGTGGAGGTGGTGGAGATGCATGAGAGCGACATCTCGGCATACACCTACGAGAAGACATTAGTAATGGAG
CAACGATCTCAGATCCTCAAACAGATGCACCTCACCAAGAACGAGCGGGAACGGGAGATCCAGAGCATCA
CAGACGAGTCTCGGGGCTCCATTCGGAGGAAGAATCCAGCCAACCCCCGGCTCCGCCTCAATGTTCCCGA
AGAGACAGCGTGTGACAATGAGGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCATGACCAGAGTGCTCCC
AGCTGCCCTAGCAGCTCGCCATCTCCAGGGGAGGAGCCCGAGGGGGAGAGGGAGACAGACCCAGAGGTGC
ATCTTACCTGGACCAAGGATAAGTCAGTGGCAGAGAAGAATAAAGGCCCCAGTCCCGTCTCCTCCGAGGG
CATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAAAACTTGAACCAGTCCAATGTACGGCGCATGCAC
ACAGCTGTGCGGCTGAACGAGGTCATCGTGAATAAATCTCGGGATGCCAAGCTAGTTTTGCTCAACATGC
CCGGGCCTCCCCGCAACCGCAATGGGGATGAAAACTACATGGAATTCTTGGAGGTCCTCACTGAGCAACT
GGACCGGGTGATGCTGGTCCGCGGTGGCGGCCGAGAGGTCATCACCATCTACTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_020333
Insert Size 3348 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020333.2, NP_065066.2
RefSeq Size 6042 bp
RefSeq ORF 3348 bp
Locus ID 57138
UniProt ID Q91V14
Gene Summary Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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