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Slc12a5 (NM_020333) Mouse Tagged ORF Clone
CAT＃: MR211653
- TrueORF®
Slc12a5 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5)
ORF Plasmid: tGFP
"NM_020333" in other vectors (4)
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CNY 7,768.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KCC2; mKIAA1176 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211653 representing NM_020333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCCGGTGATGGCAACCCCAAAG AGAGCAGTCCCTTCATCAACAGCACGGACACGGAGAAGGGCAGAGAGTACGATGGCAGGAACATGGCCCT GTTTGAGGAGGAGATGGACACCAGCCCCATGGTATCCTCCCTGCTCAGTGGGCTGGCCAACTACACCAAC CTACCCCAGGGAAGTAGAGAGCATGAAGAAGCAGAAAATAATGAGGGTGGAAAAAAGAAGCCGGTGCAGG CTCCTCGAATGGGCACCTTCATGGGTGTGTACCTGCCGTGCCTGCAGAACATCTTTGGTGTCATCCTCTT CCTGCGGCTCACGTGGGTGGTGGGCATCGCGGGCATCATGGAGTCCTTCTGTATGGTCTTCATTTGCTGC TCCTGTACGATGCTCACAGCCATTTCCATGAGTGCAATCGCAACCAATGGTGTTGTGCCTGCTGGTGGCT CGTACTACATGATTTCCAGGTCTCTGGGCCCGGAGTTTGGGGGCGCCGTGGGCCTCTGCTTCTACCTGGG CACCACCTTTGCTGGGGCTATGTACATCCTTGGCACGATCGAGATCCTGCTGGCTTATCTCTTCCCAGCT ATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGAGGCGGCCGCCATGCTGAACAACATGCGGGTGTATG GCACCTGTGTGCTCACCTGCATGGCCACCGTTGTCTTTGTGGGTGTCAAGTACGTCAACAAGTTTGCCTT GGTCTTCCTGGGTTGCGTCATCCTGTCCATCCTGGCCATCTATGCAGGGGTCATCAAGTCTGCCTTCGAC CCACCCAATTTCCCGATCTGCCTCCTGGGGAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTGTGCCA AGCTGGCTTGGGAAGGAAATGAGACAGTGACCACACGGCTCTGGGGCCTTTTCTGCTCCTCCCGCCTCCT CAATGCCACCTGTGATGAGTACTTCACCCGAAACAATGTCACAGAGATCCAGGGCATTCCTGGTGCTGCC AGTGGTCTCATCAAAGAGAACCTGTGGAGTTCTTACCTGACCAAAGGGGTGATTGTCGAGAGGCGTGGGA TGCCCTCTGTGGGCCTGGCAGACGGTACCCCCGTAGACATGGACCACCCCTATGTCTTCAGTGATATGAC CTCCTACTTCACCCTGCTCGTTGGTATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGCTCAAACCGA TCTGGAGACCTGCGGGATGCCCAGAAGTCTATCCCTACTGGAACTATCCTGGCCATTGCTACCACCTCTG CTGTCTACATCAGCTCTGTTGTTCTGTTTGGAGCCTGCATCGAGGGGGTCGTCTTACGGGACAAGTTTGG GGAAGCTGTGAATGGCAACTTGGTGGTGGGCACCCTGGCCTGGCCTTCTCCCTGGGTCATCGTCATAGGC TCTTTCTTCTCTACCTGTGGGGCTGGATTACAGAGCCTCACAGGGGCCCCACGTCTGCTGCAGGCCATCT CCCGGGATGGCATAGTGCCCTTCCTGCAGGTCTTTGGCCATGGCAAAGCTAATGGAGAGCCAACCTGGGC GCTGCTGCTGACTGCCTGCATCTGTGAGATCGGCATCCTCATAGCCTCCCTGGATGAGGTCGCCCCTATA CTTTCCATGTTCTTCCTAATGTGTTACATGTTTGTGAACTTGGCTTGTGCGGTGCAGACGCTGCTGAGGA CACCCAACTGGAGGCCACGATTTCGCTATTACCACTGGACTCTCTCCTTCCTGGGCATGAGCCTCTGCCT GGCCCTCATGTTCATTTGCTCCTGGTACTACGCACTGGTGGCCATGCTCATTGCCGGACTCATTTATAAG TACATCGAGTACCGGGGGGCGGAGAAGGAGTGGGGGGATGGAATCCGAGGCCTGTCTCTCAGTGCAGCAC GCTATGCTCTCTTGCGCCTGGAGGAAGGACCTCCGCATACGAAGAACTGGAGGCCCCAGCTGCTGGTGCT GGTGCGTGTGGACCAGGATCAGAACGTGGTGCATCCGCAGCTGCTCTCCCTGACCTCCCAGCTCAAGGCA GGGAAGGGCCTGACCATTGTGGGCTCCGTCCTTGAGGGCACCTTTCTGGACAACCATCCACAGGCTCAGC GGGCAGAGGAGTCTATCAGGCGCCTGATGGAGGCTGAGAAGGTGAAGGGCTTCTGCCAGGTAGTGATCTC CTCCAACCTGCGTGATGGTGTGTCCCACCTGATCCAGTCTGGGGGCCTCGGGGGATTGCAACACAATACC GTGCTGGTGGGCTGGCCTCGCAACTGGAGGCAGAAGGAGGATCATCAGACATGGAGGAACTTCATCGAAC TGGTCCGGGAAACTACAGCCGGCCACCTCGCCCTGCTGGTCACCAAGAATGTTTCCATGTTTCCCGGGAA CCCTGAGCGCTTCTCGGAGGGCAGCATTGACGTGTGGTGGATTGTGCACGACGGGGGCATGCTCATGCTG CTGCCCTTCCTGCTGCGACACCACAAGGTCTGGAGGAAATGCAAAATGCGGATCTTCACCGTGGCCCAGA TGGACGATAACAGTATCCAGATGAAGAAGGACCTGACCACGTTTCTGTACCACTTACGCATTACTGCAGA GGTGGAGGTGGTGGAGATGCATGAGAGCGACATCTCGGCATACACCTACGAGAAGACATTAGTAATGGAG CAACGATCTCAGATCCTCAAACAGATGCACCTCACCAAGAACGAGCGGGAACGGGAGATCCAGAGCATCA CAGACGAGTCTCGGGGCTCCATTCGGAGGAAGAATCCAGCCAACCCCCGGCTCCGCCTCAATGTTCCCGA AGAGACAGCGTGTGACAATGAGGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCATGACCAGAGTGCTCCC AGCTGCCCTAGCAGCTCGCCATCTCCAGGGGAGGAGCCCGAGGGGGAGAGGGAGACAGACCCAGAGGTGC ATCTTACCTGGACCAAGGATAAGTCAGTGGCAGAGAAGAATAAAGGCCCCAGTCCCGTCTCCTCCGAGGG CATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAAAACTTGAACCAGTCCAATGTACGGCGCATGCAC ACAGCTGTGCGGCTGAACGAGGTCATCGTGAATAAATCTCGGGATGCCAAGCTAGTTTTGCTCAACATGC CCGGGCCTCCCCGCAACCGCAATGGGGATGAAAACTACATGGAATTCTTGGAGGTCCTCACTGAGCAACT GGACCGGGTGATGCTGGTCCGCGGTGGCGGCCGAGAGGTCATCACCATCTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211653 representing NM_020333
Red=Cloning site Green=Tags(s) MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTN LPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICC SCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPA MAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFD PPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAA SGLIKENLWSSYLTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNR SGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIG SFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPI LSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYK YIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKA GKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNT VLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLML LPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVME QRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANPRLRLNVPEETACDNEEKPEEEVQLIHDQSAP SCPSSSPSPGEEPEGERETDPEVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMH TAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020333 |
| ORF Size | 3345 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020333.2, NP_065066.2 |
| RefSeq Size | 6042 bp |
| RefSeq ORF | 3348 bp |
| Locus ID | 57138 |
| UniProt ID | Q91V14 |
| MW | 124 kDa |
| Gene Summary | Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223609 | Slc12a5 (untagged) - Mouse solute carrier family 12, member 5 (Slc12a5), (10ug) |
CNY 14,250.00 |
|
| MG211653 | Slc12a5 (tGFP-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5) |
CNY 9,368.00 |
|
| MR211653L3 | Lenti ORF clone of Slc12a5 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5) |
CNY 11,690.00 |
|
| MR211653L4 | Lenti ORF clone of Slc12a5 (mGFP-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5) |
CNY 11,690.00 |
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