Nlrp5 (NM_001039143) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Mat; Mater; N; Nalp5; O; Op1; PAN11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC223552 representing NM_001039143 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCCTCCAGAAAAAGAAAGTAAAGCAATCTTGAAAGCACGTGGATTGGAAGAGGAACAGAAGTCAG AAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCAGAAAGACCAAGGACCAGAGCAGGAGCA GACATCAGAAAGCACAATGGGTCCTCCAGAAAAAGACAGTAAAGCAATCTTGAAAGCACGTGGATTGGAA GAGGAACAGAAGTCAGAAAGCACAATGTCTCCTTCAGAAAATGTCAGTAGAGCAATCCTGAAAGACAGTG GATCAGAAGAAGTGGAACAGGCGTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCA GAAAGACCAAGGACCAGAGCAGGAGCAGACATCAGATAATGGAGGTGACTTACAAGACTACAAGGCCCAT GTGATTGCTAAGTTCGACACAAGTGTGGATCTACACTATGACAGCCCAGAGATGAAATTATTGTCTGATG CTTTTAAACCATACCAGAAAACCTTCCAGCCTCACACCATTATCCTACATGGAAGACCAGGAGTTGGGAA GTCAGCTTTGGCCAGAAGTATTGTTCTTGGCTGGGCACAGGGTAAACTCTTCCAAAAAATGTCCTTTGTC ATCTTCTTCTCTGTTAGAGAAATAAAGTGGACAGAGAAGAGCAGTTTGGCACAGCTGATTGCTAAGGAGT GTCCAGACTCCTGGGATCTAGTGACAAAGATCATGTCCCAACCAGAAAGACTCTTGTTTGTCATAGATGG CTTGGATGATATGGACTCTGTCCTCCAACATGATGATATGACACTATCCAGAGACTGGAAGGATGAACAG CCCATATACATCCTGATGTACAGCCTCCTGAGGAAGGCTCTCCTACCTCAGTCCTTTCTCATCATTACCA CCAGAAACACAGGCTTAGAAAAACTCAAGTCAATGGTTGTGTCCCCCCTCTATATACTGGTTGAAGGACT GTCTGCATCAAGGAGATCTCAGCTGGTCCTCGAGAACATCTCCAATGAGTCTGATAGAATACAAGTCTTC CATTCTCTGATAGAAAATCACCAGCTGTTTGACCAATGCCAGGCCCCCTCTGTGTGCTCCCTGGTCTGTG AGGCTCTACAGCTACAGAAGAAACTGGGAAAGAGATGCACCCTACCCTGCCAGACTCTCACCGGTTTGTA TGCCACGTTGGTGTTTCACCAGCTCACCTTGAAAAGGCCTTCCCAGAGCGCTCTCAGTCAGGAAGAACAG ATTACTCTAGTGGGTTTGTGCATGATGGCAGCTGAAGGAGTGTGGACCATGAGGTCGGTGTTCTATGATG ATGACCTGAAGAACTATAGCCTAAAGGAGTCTGAGATCTTGGCCCTCTTTCACATGAACATCCTTCTCCA GGTTGGCCACAACAGTGAGCAGTGTTATGTTTTCTCCCACCTCAGCCTGCAGGATTTCTTTGCTGCCTTA TATTATGTTTTAGAAGGGCTGGAGGAATGGAATCAGCATTTTTGCTTCATTGAAAACCAAAGGAGCATCA TGGAGGTGAAGAGAACTGACGACACTCGCCTCCTCGGGATGAAGCGTTTCTTATTTGGCCTCATGAACAA GGATATCTTGAAGACTCTGGAGGTTCTGTTTGAATATCCCGTGATTCCAACTGTTGAGCAGAAGCTCCAA CACTGGGTCTCTCTGATAGCTCAGCAGGTCAATGGCACCAGCCCAATGGACACCCTGGATGCCTTCTATT GTCTATTTGAGTCTCAGGATGAAGAGTTTGTTGGCGGGGCTCTCAAACGCTTCCAAGAAGTGTGGCTGCT GATTAACCAGAAGATGGACTTGAAGGTCTCTTCCTACTGTCTCAAGCACTGTCAGAACTTGAAGGCAATC CGGGTGGATATCAGAGACCTCCTCTCGGTAGATAATACTCTCGAGCTGTGCCCTGTTGTTACTGTCCAGG AGACACAATGTAAGCCCCTCCTCATGGAGTGGTGGGGAAACTTCTGCTCTGTGCTTGGCAGCCTCCGGAA CTTGAAGGAGCTGGACTTGGGCGACAGCATCCTGAGTCAACGGGCCATGAAGATACTGTGCCTCGAGCTG CGGAATCAGTCCTGCAGAATACAGAAGCTGACGTTTAAGAGTGCAGAGGTAGTGTCTGGCCTGAAACATC TCTGGAAGCTCCTTTTTAGCAATCAAAACTTAAAGTACCTCAATCTAGGGAACACTCCCATGAAGGATGA TGACATGAAGTTAGCCTGCGAAGCGCTGAAACATCCAAAGTGCTCCGTGGAGACTCTGAGGTTGGATTCC TGTGAGTTAACCATCATTGGTTATGAGATGATCTCCACGCTTCTTATTTCAACCACCAGGCTAAAGTGTC TCAGCCTGGCCAAAAATAGAGTGGGAGTAAAAAGCATGATATCCCTTGGGAATGCCTTGAGTAGCTCAAT GTGTCTACTGCAAAAGTTGATACTGGACAACTGTGGCCTCACACCTGCCAGCTGCCACCTTCTGGTCTCA GCCCTTTTCAGCAACCAGAACTTGACACACCTGTGCCTGTCAAACAACAGCCTGGGGACTGAAGGAGTGC AACAGCTGTGTCAGTTCCTGAGGAATCCAGAATGTGCTCTCCAGCGGCTGATACTGAATCACTGCAACAT TGTAGATGATGCTTATGGCTTCCTGGCAATGAGACTTGCAAACAACACAAAGCTGACCCACCTGAGCCTG ACCATGAACCCCGTAGGGGATGGTGCAATGAAGCTACTGTGTGAAGCTTTAAAGGAACCTACTTGTTACC TTCAAGAACTGGAACTAGTGGACTGCCAACTCACACAGAACTGCTGCGAGGACCTGGCCTGTATGATCAC AACAACCAAGCACTTAAAAAGTTTGGATCTTGGTAACAACGCCCTGGGTGACAAAGGAGTCATAACCCTG TGTGAGGGACTGAAGCAAAGTAGCAGCTCCCTGAGGAGACTTGGGTTGGGGGCATGTAAGTTGACTTCCA ATTGCTGTGAGGCATTGTCATTGGCCATCTCTTGCAACCCTCACCTGAACAGCCTAAACCTGGTGAAGAA TGACTTCAGTACATCGGGGATGTTGAAGCTGTGCTCTGCGTTCCAATGCCCTGTCTCTAACCTGGGGATA ATTGGCCTGTGGAAGCAGGAGTACTATGCCCGAGTGAGAAGACAGCTGGAGGAAGTTGAGTTTGTCAAGC CCCACGTGGTGATTGATGGTGATTGGTATGCTAGTGATGAAGATGACCGAAACTGGTGGAAAAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001039143
Insert Size	3288 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039143.2, NP_001034232.1
RefSeq Size	3473 bp
RefSeq ORF	3288 bp
Locus ID	23968
UniProt ID	Q9R1M5
Gene Summary	This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a.

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