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Phf2 (NM_011078) Mouse Untagged Clone
CAT＃: MC223534
Phf2 (untagged) - Mouse PHD finger protein 2 (Phf2), (10ug)
CNY 14,060.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | GRC5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223534 representing NM_011078
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGTGCCCGTGTACTGCGTCTGCCGGCTGCCCTACGACGTTACCCGCTTTATGATCGAGTGCG ATGCCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGGGTGGAAGAGGAAGAGGCACCAGACATCGACAT TTACCACTGCCCGAACTGCGAGAAAACCCATGGCAAGTCCACACTCAAGAAAAAGCGGACTTGGCACAAA CACGGCCCTGGGCCAACACCGGACGTGAAACCAGTGCAGAATGGCAGTCAGCTGTTCATCAAGGAGCTGC GGAGCCGAACCTTCCCCAGTGCTGAAGACGTGGTGTCCCGTGTGCCAGGTAGCCAGCTCACCGTGGGCTA CATGGAGGAGCATGGCTTCACTGAGCCCATCCTTGTCCCCAAGAAAGATGGCCTGGGCTTAGCTGTCCCT GCCCCAACATTCTACGTGAGTGATGTGGAGAACTATGTGGGGCCGGAGAGGAGTGTGGATGTGACAGACG TTACCAAACAGAAAGACTGCAAAATGAAGCTGAAAGAATTCGTGGACTATTACTATAGCACGAACCGCAA GCGTGTTCTCAACGTGACCAACCTCGAGTTCTCAGACACCAGAATGTCCAGCTTTGTGGAGCCACCTGAC ATTGTGAAGAAACTATCGTGGGTGGAAAACTACTGGCCCGATGACGCGCTGCTGGCCAAGCCCAAGGTGA CCAAGTACTGCCTGATCTGTGTGAAGGACAGCTACACCGACTTCCACATTGACTCTGGAGGTGCCTCAGC GTGGTACCATGTCCTCAAGGGGGAGAAGATCTTCTATCTCATCCGGCCAGCCTCAGCCAACATCTCCCTA TATGAGCGCTGGCGGTCAGCCTCCAACCACAGCGAGATGTTCTTCGCCGACCAGGTGGACAGGTGCTATA AATGCACTGTCAAGCAGGGCCAGACCCTCTTCATCCCCTCAGGCTGGATCTATGCCACACTCACCCCCGT GGACTGCCTGGCCTTCGCTGGACATTTCCTCCACAGCCTCAGTGTGGAGATGCAGATGAGGGCATACGAG GTGGAAAGGAGGTTAAAACTTGGCAGCCTGACTCAGTTTCCCAACTTTGAAACTGCCTGCTGGTACATGG GAAAGCACCTATTGGAAGCGTTCAAAGGTTCTCACAAGTCTGGGAAACAGCTGCCCCCACACTTAGTTCA AGGAGCTAAAATTCTCAATGGTGCTTTTAGGTCATGGACGAAGAAGCAGGCTTTGGCAGAGCATGAGGAC GAACTCCCAGAGCACTTCAGGCCCTCACAGCTCATCAAGGACCTGGCCAAAGAGATCAGGCTCAGTGAGA ATGCCTCCAAAACTGTCCGACCTGAAGTAAACGCTGCCGCCTCCTCAGATGAGGTCTGTGATGGAGACCG GGAGAAGGAAGAACCGCCATCTCCTGTTGAGACTACCCCACCTCGATCCCTCCTGGAGAAAGTGTCCAAA AAAAAGACTTCCAAGACTGTGAAGATGCCCAAGCCATCCAAAATTCCCAAGCCCCCCAAATCCCCCAAGC CCCCCAAAACTCTGAAGCTCAAAGATGGGAGCAAGAAGAAAGGGAAGAAGTGCAAGGAGTCAGCCTCACC CACCATCCCCAACCTAGACCTGCTTGAGGCTCACACAAAGGAGGCGCTGACCAAGATGGAGCCACCGAAG AAAGGGAAGACCCCAAAGAGCGTCCTGAGTGTTCCCAATAAGGACACAGTCCACACGCAGAATGACATGG AAAGGCTGGAAATTCGAGAGCAAACAAAGAGCAAGTCAGAAGCCAAGTGGAAATACAAGAACAGCAAACC TGACTCGTTACTGAAGATGGAGGAGGAGCAGAGGCTGGAGAAGTCGCCCCTGGCTGGGAACAAGGACAAG TTTTCCTTTTCTTTCTCCAACAGAAAACTCCTGGGCTCCAAGGCCCTCAGGCCCCCGAGCAGCCCTGGTG TGTTCGGCGCCTTGCAGAGCTTCAAGGAGGACAAGGCCAAGCCCGTGCGCGATGAGTATGAGTACGTATC AGATGATGGGGAGCTGAAGATAGACGAGTTTCCCATCAGGAGGAAGAAGAGCGCCCCCAAAAGGGACTTG TCCTTCTTGTTAGACAAGAAGGAGGCTCTCCTCATGCCCACCTCGAAGCCAAAGCTGGATTCTGCGGTGT ACAAGAGCGATGACTCCTCTGACGAGGGCTCTCTGCACATCGACACGGACACCAAGCCAGGCAGAAATGC CAAAGTGAAGAAGGAGAGTGGGAGCTCCGCGGCCGGCATCCTGGACCTGCTGCAGGCCAGCGAGGAGGTT GGCGCACTCGAGTACAACCCCAACAGCCAGCCCCCTGCCTCCCCCAGCACACAGGAAGCCATTCAGGGAA TGCTCTCCATGGCCAATCTGCAGGCCTCTGACTCTTGCCTGCAGACCACATGGGGCACGGGGCAGGCCAA GGGTGGCTCACTGGCAGCCCATGGTGCCCGGAAGATTGGTGGTGGCAACAAAGGCACAGGCAAGCGCCTG CTGAAGAGGACTGCCAAGAACAGTGTGGATCTGGAGGACTACGAGGAGCAGGATCACCTGGATGCCTGCT TCAAGGACTCAGACTATGTTTACCCCTCACTGGAGTCTGACGAAGATAACCCCGTCTTCAAGTCCCGGTC AAAGAAGAGGAAAGGCTCAGACGATGCTCCGTACAGCCCCACAGCCAGGGTCGGTCCATCGGTGCCAAGA CAAGACAGGCCTGTGCGTGAGGGGACCAGAGTGGCCTCCATTGAGACGGGGCTGGCAGCTGCTGCAGCCA AGCTGTCCCAGCAGGAGGAGCAGAAAAACAGGAAGAAGAAGAACACCAAAAGGAAGCCGGCTCCTAACAC TGCCTCCCCCTCCATCTCCACCTCTGCCTCCGCCTCCACGGGTACCACCTCGGCCTCCACCACCCCAGCA TCCACCACCCCGGCCTCCACCACCCCAGCATCCACCACCCCGGCCTCCACCAGCACAGCCAGCAGCCAGG CCTCACAGGAGGGCAGCTCACCTGAGCCCCCACCTGAATCACACAGCAGTAGCCTGGCTGACCACGAATA TACAGCAGCCGGCACATTCTCGGGGTCCCAGGCTGGCCGTGCCTCCCAGCCCATGGCCCCTGGAGTCTTT CTCACACAGAGGCGGCCTTCTGCATCATCCCCCAACAACACTGCTGCCAAAGGAAAACGTACAAAAAAGG GCATGGCCACCGCCAAGCAAAGGCTTGGAAAGATCTTGAAGATCCATCGGAACGGGAAACTGCTCCTCTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011078 |
| Insert Size | 3291 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011078.3, NP_035208.2 |
| RefSeq Size | 5260 bp |
| RefSeq ORF | 3291 bp |
| Locus ID | 18676 |
| UniProt ID | Q9WTU0 |
| Gene Summary | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211626 | Phf2 (tGFP-tagged) - Mouse PHD finger protein 2 (Phf2) |
CNY 10,550.00 |
|
| MR211626 | Phf2 (Myc-DDK-tagged) - Mouse PHD finger protein 2 (Phf2) |
CNY 7,632.00 |
|
| MR211626L3 | Lenti ORF clone of Phf2 (Myc-DDK-tagged) - Mouse PHD finger protein 2 (Phf2) |
CNY 11,500.00 |
|
| MR211626L4 | Lenti ORF clone of Phf2 (mGFP-tagged) - Mouse PHD finger protein 2 (Phf2) |
CNY 11,500.00 |
