Cacna2d1 (NM_009784) Mouse Untagged Clone

CAT#: MC223473

Cacna2d1 (untagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e, (10ug)



  "NM_009784" in other vectors (4)

CNY 13,870.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Ca(v)alpha2delta1; Cac; Cacna2; Cchl; Cchl2a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223473 representing NM_009784
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTGGGCTGATCGGCCCCTCGAGCG
AGGAGCCCTTCCCTTCGCCCGTCACTATCAAGTCATGGGTGGACAAGATGCAAGAAGACCTTGTCACACT
GGCAAAAACAGCAAGTGGAGTGACTCAGCTTGCTGATATTTATGAAAAATACCAAGATTTGTATACTGTG
GAGCCCAACAATGCACGCCAACTGGTTGAAATTGCAGCCCGAGACATTGAGAAGCTTCTAAGCAACAGAT
CTAAGGCCCTGGTGCGCCTGGCTATGGAAGCAGAGAAAGTCCAAGCAGCCCACCAATGGAGGGAAGATTT
TGCAAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCA
GGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATG
CAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAG
TGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGC
GCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTG
ATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCT
GGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTA
GAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTT
TCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGC
AAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCC
AGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTG
CCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGG
ACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGA
ATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAG
TTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTT
CAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTT
GATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTG
CAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCC
AGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATA
GATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAG
GAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTA
CAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATATTCAGAAACCCTGAAG
CCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTT
CAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTC
CTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTAT
TGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAG
TTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACG
GAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAA
TCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGG
GAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGG
TCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTT
TTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGA
TGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCC
AGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAG
ATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCAC
GGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCAC
AGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGT
TCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGA
CATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACAT
GGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGGAGGATTATACT
GACTGTGGTGGTGTTTCTGGGTTAAACCCTTCCTTATGGTCTATCTTTGGACTCCAGTTTATACTCCTTT
GGCTGGTATCTGGCAGCAGACACTACCTACTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009784
Insert Size 3255 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009784.2, NP_033914.1
RefSeq Size 7415 bp
RefSeq ORF 3255 bp
Locus ID 12293
UniProt ID O08532
Gene Summary This gene encodes a regulatory component of the voltage-dependent calcium channel complex. The product of this gene is a proprotein that is proteolytically processed into alpha-2 and delta subunits, which are linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Transcript Variant: This variant (e) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (e) is shorter than isoform a, and is processed into alpha-2e and delta-1 subunits. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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