Sema5a (NM_009154) Mouse Untagged Clone

CAT#: MC223449

Sema5a (untagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10ug)



  "NM_009154" in other vectors (4)

CNY 13,780.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5930434A13; 9130201M22Rik; AI464145; Semaf; semF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223449 representing NM_009154
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTAGGCCCGAGA
CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCTGTCGTCTCTTACAAAGAAATTGGCCCCTGGTT
ACGGGAGTTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT
GTCGTAGGAGCGAGAAACTATCTCTTCAGATTAGAGCTTGAGGATCTGTCTCTCATCCAGGCTGTGGAGT
GGGAGTGTGACGAAGCCACCAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA
CATACGAGTGCTCTTAGTTGGTGGGGACCGGCTATTCACCTGTGGGACCAATGCCTTCACACCTGTCTGT
ACCATCCGCTCGTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA
GTCCCCAGCACAATTCTACCGCCCTACTCACTGCCAGTGGTGAGCTCTATGCTGCAACAGCCATGGATTT
CCCAGGGCGAGATCCAGCCATTTACCGAAGTCTGGGCACTTTGCCTCCTCTTCGAACTGCACAGTACAAC
TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGAAATTTCACCTACTTCTTCTTCC
GAGAAAACGCCGTGGAACATGACTGTGGGAAGACGGTGTTCTCCAGGGCTGCCCGGGTCTGTAAAAATGA
CATTGGAGGGCGATTTCTTCTGGAGGACACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCCCGG
CCTGGTGAGGTGCCCTTCTACTACAATGAGCTGCAGGGTACTTTCTTCCTGCCGGAGCTGGATCTGATCT
ATGGTATCTTCACCACCAATGTAAACAGCATCGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT
CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAAAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC
CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTACGTAAACCTGACGGAAAGAAACCTAC
AGGATGCTCAGAAGTTCATTCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCCTCTTTCATGGA
GGACAACAGCCGCTTCTCCCACTTAGCTGTTGATGTTGTGCAAGGCAGGGAGACTCTGGTCCACATCATT
TATCTGGCCACAGATTATGGCACCATTAAGAAAGTACGAGCTCCCCTGAGTCAGAGCTCAGGCAGCTGTT
TGCTGGAAGAGATTGAGCTTTTCCCAGAGAGGAGGAGTGAGCCCATCAGGAGCCTGCAGATCCTCCACAG
CCAGAGTGTCCTATTTGTGGGACTACAGGAGCATGTGGCCAAGATCCCCCTGAAGAGGTGCCACTTCCAT
CAAACACGCAGTGCCTGCATTGGCGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAATGCA
CCAGCCTGGAGGAGAGTCTGAGCATGACCCAGTGGGATCAGAGCATCCCCACCTGTCCGACCAGAAATCT
CACTGTAGATGGGAGCTTTGGCCCATGGTCACCGTGGACACCCTGTACACACACTGATGGCACTGCTGTG
GGCTCCTGCCTCTGCCGGTCCCGCTCCTGTGACAGCCCAGCTCCTCAGTGTGGTGGTTGGCAGTGTGAGG
GCCCTAGAATGGAGATCACCAACTGTTCCAGGAATGGAGGCTGGACTCCCTGGACCTCCTGGTCTCCGTG
CAGCACGACCTGTGGCATTGGCTTCCAAGTGCGGCAGCGATCCTGCAGCAACCCCACGCCCAGGCATGGC
GGGCGCGTGTGCGTGGGCCAGAACAGGGAGGAAAGATACTGCAATGAACATTTGCTCTGCCCACCACACG
TGTTCTGGACAGGCTGGGGACCTTGGGAACGGTGCACAGCCCAGTGCGGGGGTGGCATTCAAGCCCGCCG
GAGGACCTGTGAAAATGGGCCTGACTGTGCAGGATGCAATGTGGAATACCAGCCTTGTAACACCAATGCA
TGCCCGGAGCTGAAGAAGACCACACCCTGGACACCCTGGACCCCTGTCAACATCTCTGACAATGGAGGTC
ACTATGAGCAGCGTTTCCGCTATACCTGTAAAGCTCGCCTGCCAGATCCAAATTTGCTGGAAGTAGGAAG
ACAGAGGATAGAAATGCGGTACTGTTCCAGCGATGGAACCAGTGGCTGCTCCACAGACGGACTTTCTGGA
GACTTTCTAAGAGCTGGGAGATACTCTGCTCATACAGTCAATGGGGCATGGTCAGCCTGGACTTCCTGGT
CACAGTGCAGCAGAGACTGCAGCAGGGGCATTCGGAACCGGAAGCGTGTTTGCAACAACCCAGAACCCAA
GTTTGGGGGCATGCCATGCCTTGGCCCATCGCTGGAGTTCCAGGAATGCAACATTTTACCTTGTCCAGTG
GATGGTGTGTGGTCTTGCTGGTCATCCTGGTCTAAATGTTCAGCAACCTGTGGAGGTGGCCACTACATGA
GAACCCGTTCTTGTTCGAATCCAGCCCCAGCATATGGAGGGGACATCTGCCTGGGACTGCACACAGAAGA
GGCACTCTGCAACACACAGACCTGCCCAGAAAGCTGGTCAGAGTGGTCAGACTGGTCTGTGTGCGATGCA
TCTGGTACCCAGGTCCGTGCTCGGCAGTGCATCCTTCTGTTTCCAGTGGGCAGCCAATGTTCTGGAAATA
CTACAGAGAGCCGGCCTTGTGTATTTGACTCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGCAG
CGTAGAAGAAAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATGGCCGTGGGGCTTAGCAGTTCCATT
CTTGGCTGCCTCCTCACACTGCTTGTCTACACCTACTGCCAGAGGTACCAGCAGCAGTCCCATGATGCAA
CTGTCATCCACCCTGTCTCTCCTGCCGCCCTCAACAGCAGCATAACTAACCACATCAACAAACTGGACAA
ATATGATTCTGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAGAGAAACAAATAC
TTCAACCCACATCTCACTGGGAAGACCTATTCCAACGCCTACTTTACAGATCTCAACAATTATGATGAAT
ACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009154
Insert Size 3225 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009154.2, NP_033180.2
RefSeq Size 10809 bp
RefSeq ORF 3225 bp
Locus ID 20356
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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