Pik3cb (NM_029094) Mouse Untagged Clone

CAT#: MC223419

Pik3cb (untagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb), (10ug)



  "NM_029094" in other vectors (4)

CNY 13,680.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110001J02Rik; AI447572; p110beta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223419 representing NM_029094
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCTGCTATGGCAGACAACCTTGACATCTGGGCAGTGGACTCACAGATTGCATCCGATGGCGCCA
TATCCGTCGATTTCCTTCTGCCCACCGGGATTTATATCCAGTTGGAAGTACCTCGGGAAGCTACCATTTC
TTATATTAAACAGATGTTATGGAAGCAAGTTCACAACTACCCGATGTTTAACCTCCTCATGGACATTGAC
TCGTATATGTTTGCATGTGTGAATCAAACTGCTGTATATGAGGAACTGGAAGACGAAACACGAAGACTTT
GTGATGTCAGACCTTTTCTTCCAGTTCTCAAACTAGTGACTAGAAGCTGTGACCCCGCAGAAAAATTGGA
CTCAAAAATTGGGGTTCTTATAGGAAAAGGTCTTCATGAGTTTGATGCCTTGAAGGATCCCGAAGTGAAT
GAATTTAGAAGAAAAATGCGCAAATTCAGTGAGGCCAAGATTCAGTCTCTGGTAGGGTTGTCTTGGATCG
ACTGGCTAAAGCACACGTATCCGCCTGAGCACGAGCCGTCCGTCCTGGAGAACTTGGAAGATAAACTTTA
TGGAGGAAAGCTGGTTGTGGCTGTGCACTTTGAAAATAGCCAGGATGTATTTAGTTTTCAAGTGTCTCCC
AATTTGAATCCTATAAAAATAAATGAATTGGCAATCCAGAAACGCCTCACTATTCGTGGAAAGGAAGATG
AAGCTAGCCCCTGTGACTATGTGTTACAGGTCAGTGGGAGAGTGGAGTATGTGTTTGGCGATCATCCACT
AATTCAGTTCCAGTACATCCGGAATTGTGTGATGAATAGAACCCTGCCCCACTTCATCCTTGTGGAATGT
TGTAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATCAACCGAAACTCATCCA
ACCTTCCTCTCCCTTTACCACCAAAGAAAACGCGAGTTATTTCTCATATCTGGGACAACAACAACCCTTT
CCAAATTACCTTGGTTAAAGGAAATAAGCTTAATACAGAAGAAACTGTGAAAGTTCATGTCCGAGCTGGG
CTTTTTCACGGAACCGAGCTCCTGTGTAAAACCGTCGTAAGCTCAGAGATATCAGGAAAGAACGACCATA
TTTGGAATGAACAACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTATGTTTTGC
TGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACAAAGACTATTAATCCCTCTAAGTATCAG
ACCATCAGGAAAGCCGGGAAAGTGCATTATCCTGTCGCATGGGTAAATACCATGGTTTTTGACTTCAAAG
GACAGCTGAGGTCTGGAGACGTCATATTGCATAGCTGGTCTTCGTTTCCTGATGAGCTGGAAGAAATGCT
GAATCCCATGGGGACTGTGCAGACGAACCCATATGCTGAGAACGCCACCGCCTTGCACATTACGTTCCCA
GAGAATAAGAAGCAGCCGTGTTATTATCCCCCCTTCGATAAGATCATTGAGAAGGCAGCTGAGCTTGCCA
GCGGAGACAGTGCTAATGTGTCAAGTCGTGGTGGAAAAAAATTTCTTGCTGTGCTGAAAGAAATCTTGGA
CAGGGACCCCCTGTCTCAGCTGTGTGAGAACGAAATGGACCTTATTTGGACTCTACGGCAAGACTGCCGA
GAAAATTTCCCTCAGTCACTGCCAAAACTACTCTTGTCAATCAAGTGGAATAAACTTGAAGATGTTGCTC
AGCTTCAGGCGCTCCTGCAGATATGGCCCAAACTGCCCCCCAGGGAAGCCCTGGAACTCCTGGATTTCAA
CTATCCAGACCAGTATGTCCGGGAATACGCTGTAGGCTGCCTTCGACAGATGAGTGATGAAGAACTCTCT
CAGTATCTTTTACAATTGGTGCAAGTTTTGAAATATGAGCCTTTTCTCGATTGTGCCCTCTCCAGATTCC
TATTAGAAAGAGCACTTGATAATCGGAGGATTGGGCAGTTTCTGTTTTGGCATCTTAGGTCAGAGGTGCA
CACTCCTGCTGTGTCCGTACAGTTTGGTGTCATCCTGGAAGCATACTGTCGAGGAAGCGTGGGGCACATG
AAAGTGCTTTCCAAACAGGTGGAAGCACTCAATAAGTTAAAAACTTTAAATAGCTTAATCAAACTGAATG
CGGTGAAGCTGAGCAGAGCTAAGGGAAAGGAGGCCATGCACACGTGCCTGAAACAGAGTGCTTACCGGGA
GGCGCTCTCTGACCTGCAGTCGCCGCTGAACCCCTGCGTCATCCTCTCAGAGCTCTATGTTGAAAAGTGC
AAATACATGGACTCCAAGATGAAGCCCCTGTGGCTGGTCTACAGCAGCAGAGCCTTTGGAGAGGACTCGG
TTGGAGTGATCTTTAAAAATGGTGACGATTTGCGGCAGGACATGCTGACGCTGCAGATGTTGCGCCTGAT
GGATCTGCTTTGGAAAGAAGCTGGCTTGGACCTGCGGATGCTCCCCTATGGCTGCTTAGCAACAGGAGAT
CGCTCTGGCCTCATTGAGGTTGTGAGCACCTCTGAGACAATCGCTGACATTCAGCTGAACAGTAGTAACG
TGGCTGCCACGGCAGCCTTCAACAAAGACGCACTCCTGAACTGGCTCAAGGAGTACAACTCTGGGGATGA
CCTGGACCGAGCGATTGAGGAGTTTACCTTGTCCTGTGCTGGCTACTGTGTAGCCTCTTATGTCCTCGGC
ATTGGTGACAGGCACAGTGACAACATCATGGTGAAGAAAACCGGCCAGCTCTTCCACATAGATTTTGGGC
ATATTCTTGGAAATTTCAAATCTAAATTTGGCATTAAAAGGGAGCGAGTACCTTTTATTCTTACTTATGA
CTTCATTCATGTCATTCAACAAGGAAAAACGGGAAACACTGAAAAATTTGGCAGATTCCGCCAGTGCTGT
GAAGATGCGTATCTGATTTTACGGCGGCATGGGAATCTCTTCATCACCCTGTTTGCCCTGATGTTGACTG
CAGGGCTGCCTGAGCTCACATCGGTCAAAGATATACAGTATCTTAAGGACTCGCTTGCCTTAGGGAAGAG
CGAGGAGGAAGCACTGAAGCAGTTCAAGCAGAAGTTTGACGAGGCCCTCAGGGAAAGCTGGACTACTAAA
GTGAACTGGATGGCTCACACAGTACGGAAAGACTACAGGTCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029094
Insert Size 3195 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_029094.3, NP_083370.2
RefSeq Size 4854 bp
RefSeq ORF 3195 bp
Locus ID 74769
UniProt ID Q8BTI9
Gene Summary Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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