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Pik3ca (NM_008839) Mouse Untagged Clone
CAT＃: MC223413
Pik3ca (untagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca), (10ug)
CNY 13,680.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6330412C24Rik; caPI3K; p110; p110alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223413 representing NM_008839
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTTGATGCCCCCACGAATCCTAGTGGAAT GTTTACTCCCCAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCCACACTCGTCACCATCAAACA TGAACTGTTCAGAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGACGAAACTTCTTACATTTTC GTAAGTGTCACCCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTGTGACCTTCGGC TTTTTCAACCCTTTTTAAAAGTTATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCGAGAAAT TGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTCCAAGACTTTCGA AGGAACATTCTGAATGTTTGCAAAGAAGCTGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAA TGTATGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCTACAACAAGTTAGATAA AGGACAAATCATAGTGGTGATTTGGGTAATAGTCTCTCCAAACAACGACAAGCAGAAGTACACTCTGAAG ATCAATCATGACTGTGTGCCAGAGCAAGTCATTGCTGAAGCCATCAGGAAAAAGACTCGGAGCATGTTGT TGTCCTCTGAGCAGCTGAAACTCTGTGTCTTAGAATATCAGGGCAAGTATATTCTGAAAGTGTGTGGCTG TGACGAATACTTCCTGGAAAAGTACCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGG AGGATGCCCAACTTGATGCTGATGGCCAAAGAAAGCCTATACTCTCAGCTGCCGATTGATAGCTTCACCA TGCCGTCATACTCCAGGCGCATCTCCACAGCCACACCCTACATGAATGGAGAGACATCTACGAAATCCCT CTGGGTCATAAATAGTGCGCTCAGAATAAAAATTCTTTGTGCAACCTATGTAAATGTAAATATTCGAGAC ATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTC AAAGAGTACCTTGTTCCAATCCTAGGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCC TCGTGCTGCGCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGCTAAGGAGGAGCACTGT CCGTTGGCCTGGGGAAACATAAACTTGTTTGATTATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGA ATCTCTGGCCTGTACCGCATGGGTTAGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAATCCAAA TAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCAGTGTGGTGAAGTTTCCAGACATGTCT GTGATCGAAGAACATGCCAATTGGTCCGTGTCCCGAGAAGCTGGATTCAGTTACTCCCATACAGGACTGA GTAACAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCCGAGCACTTTGCACCCG GGACCCACTATCTGAAATCACTGAACAAGAGAAAGACTTCCTATGGAGCCACAGACACTACTGCGTAACT ATTCCTGAAATCCTACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGACGAAGTGGCCCAGATGT ACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCCATGGAACTCCTGGACTGTAACTATCC AGATCCTATGGTTCGGAGTTTTGCTGTTCGGTGCTTAGAAAAATATTTAACAGATGACAAACTTTCTCAG TACCTCATTCAACTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTGCTTGTGAGATTTTTAC TCAAGAAAGCATTGACAAATCAAAGGATTGGCCATTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAA TAAGACTGTCAGTCAGAGGTTTGGCCTGCTATTGGAGTCCTACTGCCGTGCCTGTGGGATGTATCTGAAG CACCTGAACAGACAAGTAGAGGCCATGGAGAAGCTCATCAACCTAACGGACATCCTTAAGCAGGAGAAGA AGGATGAGACACAAAAGGTACAGATGAAGTTTTTGGTTGAACAGATGAGACAGCCAGACTTCATGGATGC TTTGCAGGGTTTTCTGTCCCCTCTGAATCCTGCTCACCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGA ATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCAGAGCTACTGT TTCAGAACAATGAGATCATCTTTAAAAATGGCGACGACTTACGGCAAGATATGTTAACCCTTCAGATCAT CCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTCGCATGCTACCTTATGGCTGTCTATCC ATTGGGGACTGTGTGGGTCTCATCGAGGTGGTGAGAAACTCTCACACCATCATGCAAATCCAGTGCAAAG GAGGCCTGAAGGGGGCGCTGCAGTTCAACAGCCACACACTGCATCAATGGCTCAAGGACAAGAACAAGGG CGAGATATATGATGCAGCCATTGACCTGTTCACTCGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATC TTGGGAATTGGAGACCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATATAGATT TTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAGCGGGAACGTGTGCCATTTGTGTTGAC ACAGGATTTCTTGATTGTGATTAGTAAGGGAGCACAAGAGTACACCAAGACCAGAGAGTTTGAGAGGTTT CAGGAGATGTGTTACAAGGCTTACCTAGCAATTCGGCAGCATGCCAATCTCTTCATCAACCTTTTTTCAA TGATGCTTGGCTCTGGAATGCCAGAACTACAATCTTTTGATGACATTGCATATATCCGAAAGACTCTAGC CTTGGACAAAACTGAGCAAGAAGCTTTGGAATATTTCACAAAGCAAATGAATGATGCACATCATGGTGGA TGGACGACAAAAATGGATTGGATCTTCCACACCATCAAGCAGCATGCTTTGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008839 |
| Insert Size | 3207 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008839.2, NP_032865.2 |
| RefSeq Size | 8917 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 18706 |
| UniProt ID | P42337 |
| Gene Summary | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Also has serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Plays a role in the positive regulation of phagocytosis and pinocytosis (PubMed:19604150).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226580 | Pik3ca (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase catalytic alpha polypeptide (Pik3ca), (10ug) |
CNY 9,220.00 |
|
| MR226580 | Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 11,152.00 |
|
| MR226580L1 | Lenti ORF clone of Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 13,552.00 |
|
| MR226580L2 | Lenti ORF clone of Pik3ca (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
|
| MR226580L3 | Lenti ORF clone of Pik3ca (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
|
| MR226580L4 | Lenti ORF clone of Pik3ca (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) |
CNY 10,260.00 |
