Nlrp12 (NM_001033431) Mouse Untagged Clone

CAT#: MC223405

Nlrp12 (untagged) - Mouse NLR family, pyrin domain containing 12 (Nlrp12), (10ug)



  "NM_001033431" in other vectors (4)

CNY 13,590.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
Goat Anti-Nalp12 / Nlrp12 (rodent) Antibody
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Nalp12; PYPAF7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223405 representing NM_001033431
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCCGTCTACAGCCAGGGATGGCCTCTATCGACTGTCTACCTACCTGGAAGAACTCGAGGCTGGGG
AACTGAAGAAATTCAAATTATTCCTGGGGATTGCAGAGGACCTGAGCCAGGACAAAATTCCCTGGGGACG
AATGGAGAAGGCTGGTCCTCTGGAAATGGCTCAGCTGATGGTGGCCCACATGGGGACAAGGGAGGCTTGG
CTTCTGGCTCTCAGCACCTTTCAGAGGATTCACAGGAAGGACCTGTGGGAGCGAGGACAGGGAGAAGACC
TGGTGAGGGTCACTCCAAATAATGGTCTATGCCTTTTTGAGAGCCAATCAGCGTGCCCTTTGGATGTCTC
TCCCAATGCTCCAAGAAAAGATCTACAGACAACCTACAAAGACTATGTCCGAAGGAAATTCCAGCTAATG
GAAGACCGCAATGCACGATTAGGCGAATGTGTGAACCTGAGCAATCGTTACACTCGGCTTCTCCTAGTAA
AAGAACACTCAAATCCTATCTGGACACAGCAGAAATTTGTAGATGTAGAGTGGGAACGCTCCAGAACCAG
GCGTCACCAGACTAGTCCTATCCAAATGGAGACCCTCTTTGAGCCAGACGAAGAACGCCCCGAGCCACCA
CACACAGTGGTATTACAAGGGGCAGCGGGGATGGGGAAGTCCATGCTGGCCCACAAAGTGATGTTGGACT
GGGCCGATGGGAGGCTCTTCCAAGGCCGGTTTGATTATGTCTTCTATATCAGCTGCAGGGAGTTGAATAG
AAGCCACACCCAGTGCAGTGTACAAGACCTCATCTCCAGCTGCTGGCCGGAGCGTGGTATATCCCTCGAA
GACCTCATGCAGGCTCCTGACCGTCTCCTATTCATCATTGATGGCTTCGATAAACTCCATCCTTCTTTCC
ATGATGCTCAGGGTCCCTGGTGCCTCTGCTGGGAGGAGAAACAACCTACTGAAGTCCTCCTCGGAAGTCT
GATTCGGAGGTTGCTTCTGCCCCAGGTCTCTCTGCTCATCACCACACGACCCTGTGCACTGGAGAAGCTG
CACGGCTTGCTAGAACACCCCAGGCACGTGGAGATCCTGGGCTTCTCCGAGGAAGCTAGGAAGGAATATT
TCTACAGATATTTCCACAACACTGGACAAGCAAGCCGGGTGTTAAGCTTCTTGATGGACTATGAGCCCCT
CTTTACCATGTGTTTTGTTCCCATGGTGTCCTGGGTGGTCTGCACCTGCCTAAAGCAGCAGCTGGAAAGT
GGGGAGCTTTTAAGACAAACACCTAGGACCACCACAGCTGTTTATATGTTCTACCTTCTGAGCCTGATGC
AGCCCAAGCCAGGGACTCCAACCTTCAAAGTCCCAGCCAACCAGAGAGGCCTGGTCTCTCTGGCTGCAGA
GGGCCTCTGGAATCAGAAGATTCTATTTGATGAACAGGATCTTGGGAAACACGGCCTAGATGGAGCAGAT
GTGTCCACTTTCCTCAACGTGAACATATTCCAGAAGGGTATCAAATGTGAGAAATTCTACAGCTTCATCC
ACCTGAGTTTCCAGGAATTCTTCGCAGCCATGTACTGTGCACTGAATGGCAGAGAGGCGGTGAGGAGAGC
GCTGGCTGAGTATGGTTTTTCGGAAAGGAACTTCTTGGCCCTCACGGTCCACTTTCTGTTTGGCCTCCTC
AACGAAGAGATGAGATGCTACCTTGAGAGGAATCTCGGCTGGAGCATCTCCCCTCAGGTGAAGGAGGAAG
TGTTGGCATGGATCCAAAACAAGGCTGGGAGTGAAGGCTCCACCCTGCAGCATGGCTCCCTGGAGCTACT
CAGCTGCTTGTATGAGGTCCAGGAGGAGGACTTCATCCAGCAGGCCCTGAGCCACTTTCAAGTGGTTGTA
GTCAGAAGCATCTCAACAAAGATGGAGCACATGGTCTGCTCGTTTTGTGCGAGGTATTGCAGAAGTACAG
AAGTGCTTCACTTGCATGGGAGTGCTTATAGTACAGGCATGGAGGACGACCCACCAGAACCTTCAGGAGT
CCAGACTCAGTCCACATACTTACAGGAAAGGAACATGCTGCCTGATGTCTACAGTGCATACCTTTCAGCA
GCTGTCTGTACCAACTCCAACCTGATCGAGCTGGCCTTATACCGAAATGCCTTGGGCAGCCAGGGTGTAA
GGCTGCTCTGTCAAGGCCTCCGACATGCCAGCTGCAAGCTGCAGAACCTGAGGCTGAAGAGGTGTCAGAT
CTCCGGATCAGCCTGCCAGGACCTCGCAGCCGCTGTCATCGCCAACAGGAATTTAATCAGGCTGGACCTC
AGTGACAACAGCATTGGGGTGCCAGGCCTGGAGCTGCTCTGTGAGGGGCTGCAGCACCCCAGGTGTAGGC
TGCAGATGATCCAGCTGAGGAAGTGTCTGTTGGAGGCTGCAGCTGGCCGATCCCTGGCTTCTGTTCTCAG
CAACAACTCATATCTGGTAGAACTGGATCTGACAGGAAACCCCTTGGAAGATTCGGGGCTGAAGTTACTG
TGTCAAGGGCTAAGGCACCCTGTCTGCAGGCTGCGTACCCTGTGGCTGAAGATCTGCCACCTTGGACAAG
CTTCCTGCGAAGATCTGGCCTCTACTCTCAAAATGAACCAGAGCCTGCTGGAGCTGGACCTGGGTCTGAA
TGATCTTGGAGATTCTGGGGTGCTTCTGCTGTGTGAAGGCCTCAGTCATCCAGATTGCAAACTCCAGACC
CTTCGGTTGGGCATTTGCCGACTGGGCTCAGTCGCGTGTGTGGGGATCGCCAGTGTGCTCCAGGTCAACA
CATGCCTCCAAGAGCTGGACCTGAGCTTCAATGACTTGGGAGACAGGGGCCTGCAGCTGCTGGGGGAAGG
CCTGAGGCACCAGACCTGCAGACTCCAGAAGCTGTGGCTGGACAACTGCGGACTCACCTCCAAAGCATGT
GAGGACCTTTCTTCTATCCTGGGAATCAGCCAGACCCTGCATGAGCTTTATTTGACCAATAATGCTCTGG
GGGACACAGGTGTCTGTCTGCTGTGCAAGAGGCTGAGGCATCCAGGCTGCAAGCTTCGAGTCCTGTGGCT
GTTTGGGATGGACCTGAATAAAAAGACTCACAGGAGGATGGCAGCACTTCGAGTCACAAAACCGTACCTG
GATATTGGGTGTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001033431
Insert Size 3165 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001033431.1, NP_001028603.1
RefSeq Size 4329 bp
RefSeq ORF 3165 bp
Locus ID 378425
UniProt ID E9Q5R7
Gene Summary Plays an essential role as an potent mitigator of inflammation (PubMed:26521018, PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways (PubMed:30559449). Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway (PubMed:30559449). In turn, promotes bacterial tolerance (PubMed:30559449). Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation (By similarity). Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue (PubMed:30212649).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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