Tlr7 (NM_133211) Mouse Untagged Clone

CAT#: MC223375

Tlr7 (untagged) - Mouse toll-like receptor 7 (Tlr7), (10ug)



  "NM_133211" in other vectors (4)

CNY 13,490.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal TLR7 Antibody
    • 100 ug

CNY 5,808.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223375 representing NM_133211
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTTTCGATGTGGACACGGAAGAGACAAATTTTGATCTTTTTAAATATGCTCTTAGTTTCTAGAG
TCTTTGGGTTTCGATGGTTTCCTAAAACTCTACCTTGTGAAGTTAAAGTAAATATCCCAGAGGCCCATGT
GATCGTGGACTGCACAGACAAGCATTTGACAGAAATCCCTGAGGGCATTCCCACTAACACCACCAATCTT
ACCCTTACCATCAACCACATACCAAGCATCTCTCCAGATTCCTTCCGTAGGCTGAACCATCTGGAAGAAA
TCGATTTAAGATGCAATTGTGTACCTGTTCTACTGGGGTCCAAAGCCAATGTGTGTACCAAGAGGCTGCA
GATTAGACCTGGAAGCTTTAGTGGACTCTCTGACTTAAAAGCCCTTTACCTGGATGGAAACCAACTTCTG
GAGATACCACAGGATCTGCCATCCAGCTTACATCTTCTGAGCCTTGAGGCTAACAACATCTTCTCCATCA
CGAAGGAGAATCTAACAGAACTGGTCAACATTGAAACACTCTACCTGGGTCAAAACTGTTATTATCGAAA
TCCTTGCAATGTTTCCTATTCTATTGAAAAAGATGCTTTCCTAGTTATGAGAAATTTGAAGGTTCTCTCA
CTAAAAGATAACAATGTCACAGCTGTCCCCACCACTTTGCCACCTAATTTACTAGAGCTCTATCTTTATA
ACAATATCATTAAGAAAATCCAAGAAAATGATTTTAATAACCTCAATGAGTTGCAAGTTCTTGACCTAAG
TGGAAATTGCCCTCGATGTTATAATGTCCCATATCCGTGTACACCGTGTGAAAATAATTCCCCCTTACAG
ATCCATGACAATGCTTTCAATTCATTGACAGAATTAAAAGTTTTACGTTTACACAGTAATTCTCTTCAGC
ATGTGCCCCCAACATGGTTTAAAAACATGAGAAACCTCCAGGAACTAGACCTCTCCCAAAACTACTTGGC
CAGAGAAATTGAGGAGGCCAAATTTTTGCATTTTCTTCCCAACCTTGTTGAGTTGGATTTTTCTTTCAAT
TATGAGCTGCAGGTCTACCATGCATCTATAACTTTACCACATTCACTCTCTTCATTGGAAAACTTGAAAA
TTCTGCGTGTCAAGGGGTATGTCTTTAAAGAGCTGAAAAACTCCAGTCTTTCTGTATTGCACAAGCTTCC
CAGGCTGGAAGTTCTTGACCTTGGCACTAACTTCATAAAAATTGCTGACCTCAACATATTCAAACATTTT
GAAAACCTCAAACTCATAGACCTTTCAGTGAATAAGATATCTCCTTCAGAAGAGTCAAGAGAAGTTGGCT
TTTGTCCTAATGCTCAAACTTCTGTAGACCGTCATGGGCCCCAGGTCCTTGAGGCCTTACACTATTTCCG
ATACGATGAATATGCACGGAGCTGCAGGTTCAAAAACAAAGAGCCACCTTCTTTCTTGCCTTTGAATGCA
GACTGCCACATATATGGGCAGACCTTAGACTTAAGTAGAAATAACATATTTTTTATTAAACCTTCTGATT
TTCAGCATCTTTCATTCCTCAAATGCCTCAACTTATCAGGAAACACCATTGGCCAAACTCTTAATGGCAG
TGAACTCTGGCCGTTGAGAGAGTTGCGGTACTTAGACTTCTCCAACAACCGGCTTGATTTACTCTACTCA
ACAGCCTTTGAAGAGCTCCAGAGTCTTGAAGTTCTGGATCTAAGTAGTAACAGCCACTATTTTCAAGCAG
AAGGAATTACTCACATGCTAAACTTTACCAAGAAATTACGGCTTCTGGACAAACTCATGATGAATGATAA
TGACATCTCTACTTCGGCCAGCAGGACCATGGAAAGTGACTCTCTTCGAATTCTGGAGTTCAGAGGCAAC
CATTTAGATGTTCTATGGAGAGCCGGTGATAACAGATACTTGGACTTCTTCAAGAATTTGTTCAATTTAG
AGGTATTAGATATCTCCAGAAATTCCCTGAATTCCTTGCCTCCTGAGGTTTTTGAGGGTATGCCGCCAAA
TCTAAAGAATCTCTCCTTGGCCAAAAATGGGCTCAAATCTTTCTTTTGGGACAGACTCCAGTTACTGAAG
CATTTGGAAATTTTGGACCTCAGCCATAACCAGCTGACAAAAGTACCTGAGAGATTGGCCAACTGTTCCA
AAAGTCTCACAACACTGATTCTTAAGCATAATCAAATCAGGCAATTGACAAAATATTTTCTAGAAGATGC
TTTGCAATTGCGCTATCTAGACATCAGTTCAAATAAAATCCAGGTCATTCAGAAGACTAGCTTCCCAGAA
AATGTCCTCAACAATCTGGAGATGTTGGTTTTACATCACAATCGCTTTCTTTGCAACTGTGATGCTGTGT
GGTTTGTCTGGTGGGTTAACCATACAGATGTTACTATTCCATACCTGGCCACTGATGTGACTTGTGTAGG
TCCAGGAGCACACAAAGGTCAAAGTGTCATATCCCTTGATCTGTATACGTGTGAGTTAGATCTCACAAAC
CTGATTCTGTTCTCAGTTTCCATATCATCAGTCCTCTTTCTTATGGTAGTTATGACAACAAGTCACCTCT
TTTTCTGGGATATGTGGTACATTTATTATTTTTGGAAAGCAAAGATAAAGGGGTATCAGCATCTGCAATC
CATGGAGTCTTGTTATGATGCTTTTATTGTGTATGACACTAAAAACTCAGCTGTGACAGAATGGGTTTTG
CAGGAGCTGGTGGCAAAATTGGAAGATCCAAGAGAAAAACACTTCAATTTGTGTCTAGAAGAAAGAGACT
GGCTACCAGGACAGCCAGTTCTAGAAAACCTTTCCCAGAGCATACAGCTCAGCAAAAAGACAGTGTTTGT
GATGACACAGAAATATGCTAAGACTGAGAGTTTTAAGATGGCATTTTATTTGTCTCATCAGAGGCTCCTG
GATGAAAAAGTGGATGTGATTATCTTGATATTCTTGGAAAAGCCTCTTCAGAAGTCTAAGTTTCTTCAGC
TCAGGAAGAGACTCTGCAGGAGCTCTGTCCTTGAGTGGCCTGCAAATCCACAGGCTCACCCATACTTCTG
GCAGTGCCTGAAAAATGCCCTGACCACAGACAATCATGTGGCTTATAGTCAAATGTTCAAGGAAACAGTC
TAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133211
Insert Size 3153 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133211.4, NP_573474.1
RefSeq Size 3794 bp
RefSeq ORF 3153 bp
Locus ID 170743
UniProt ID P58681
Gene Summary Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks two alternate exons in the 5' region and initiates translation from an alternate upstream start codon, compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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