Pik3cd (NM_001164051) Mouse Untagged Clone

CAT#: MC223364

Pik3cd (untagged) - Mouse phosphatidylinositol 3-kinase catalytic delta polypeptide (Pik3cd), transcript variant 6, (10ug)



  "NM_001164051" in other vectors (4)

CNY 13,490.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410099E07Rik; 2610208K16Rik; AW545373; p110delta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223364 representing NM_001164051
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCCTGGGGTGGACTGCCCCATGGAGTTCTGGACCAAAGAGGAGAGCCAGAGCGTGGTTGTTGACT
TCTTGCTGCCCACAGGGGTCTACTTGAACTTCCCCGTGTCCCGCAATGCCAACCTCAGCACCATCAAGCA
GGTGCTGTGGCACCGTGCACAGTATGAGCCACTCTTCCACATGCTCAGTGACCCCGAGGCCTATGTGTTC
ACCTGTGTGAACCAGACGGCGGAGCAGCAGGAGTTGGAGGATGAGCAGCGGAGGCTGTGCGACATCCAGC
CCTTCCTGCCCGTGCTGCGCCTCGTGGCCCGAGAGGGGGACCGCGTGAAGAAGCTCATTAACTCCCAGAT
CAGCCTCCTCATTGGCAAAGGTCTCCATGAGTTTGATTCCCTGCGGGACCCGGAAGTAAACGACTTCCGC
ACTAAGATGCGCCAGTTTTGTGAAGAGGCTGCTGCTCACCGCCAGCAGCTGGGCTGGGTGGAATGGCTGC
AGTACAGCTTCCCCCTGCAGCTGGAGCCCTCAGCAAGGGGTTGGCGGGCCGGCTTATTGCGTGTCAGCAA
CCGAGCCCTGCTGGTCAACGTGAAGTTCGAGGGCAGTGAGGAGAGCTTCACCTTCCAGGTATCCACCAAG
GACATGCCCCTGGCACTGATGGCCTGTGCCCTCCGAAAAAAGGCCACAGTGTTCCGGCAGCCTCTGGTGG
AGCAGCCTGAGGAATATGCCCTGCAGGTGAACGGGAGGCACGAATACCTCTACGGCAACTACCCGCTCTG
CCACTTTCAGTACATCTGCAGCTGCCTACACAGCGGGCTGACCCCTCATCTGACCATGGTCCACTCCTCC
TCCATCCTTGCTATGCGGGATGAGCAGAGCAATCCTGCCCCCCAAGTACAGAAACCACGTGCCAAACCTC
CCCCGATCCCTGCCAAGAAGCCCTCCTCTGTGTCCCTGTGGTCCCTGGAACAGCCATTCTCCATTGAGCT
GATCGAGGGCCGAAAAGTGAATGCTGACGAGCGGATGAAGCTGGTTGTTCAGGCCGGGCTCTTCCATGGC
AATGAGATGCTGTGCAAGACTGTGTCAAGCTCGGAGGTGAATGTATGCTCAGAGCCCGTGTGGAAGCAGC
GACTGGAGTTCGATATCAGCGTCTGTGACCTCCCGCGCATGGCTCGACTCTGTTTTGCTCTCTATGCCGT
CGTGGAGAAGGCTAAGAAGGCACGCTCCACAAAGAAGAAGTCTAAGAAGGCGGACTGCCCCATCGCTTGG
GCCAACCTCATGCTATTCGACTACAAAGATCAGCTCAAGACGGGGGAGCGCTGCCTCTACATGTGGCCCT
CTGTCCCAGATGAGAAGGGAGAGCTGCTGAATCCTGCGGGTACAGTGCGCGGGAACCCCAACACGGAGAG
TGCCGCTGCCCTGGTCATCTACCTGCCTGAGGTGGCCCCCCACCCTGTGTACTTCCCCGCTCTGGAGAAG
ATCCTGGAGCTGGGGCGTCACGGGGAGCGTGGGCGCATCACGGAGGAGGAGCTGCAGCTGCGGGAGATCC
TGGAACGGCGGGGATCCGGGGAACTGTACGAACATGAGAAGGACCTGGTGTGGAAGATGCGCCACGAAGT
CCAGGAGCATTTCCCAGAGGCGCTGGCCCGCCTGCTGCTGGTCACCAAGTGGAATAAACACGAGGATGTG
GCCCAGCTGTCCCAGATGCTCTATTTGCTGTGCTCCTGGCCCGAGCTGCCTGTGCTGAGCGCCCTGGAAC
TTCTGGACTTTAGCTTTCCCGACTGCTACGTGGGCTCCTTCGCCATCAAGTCCCTTCGGAAGCTGACGGA
CGATGAGCTCTTCCAGTACCTTCTGCAGCTGGTGCAAGTGCTCAAATATGAGTCCTACCTGGACTGCGAG
CTGACCAAATTCTTGCTGGGCCGAGCCCTGGCTAACCGCAAGATCGGACACTTCCTGTTCTGGCACCTCC
GCTCTGAGATGCACGTACCATCAGTGGCTCTGCGGTTTGGTCTCATCATGGAAGCCTACTGCAGAGGCAG
CACCCACCACATGAAGGTGCTGATGAAGCAGGGGGAAGCACTGAGCAAGCTTAAGGCACTGAATGACTTT
GTGAAGGTGAGTTCCCAGAAGACCACCAAGCCCCAAACCAAGGAGATGATGCATATGTGCATGCGCCAGG
AGACCTACATGGAGGCCCTGTCCCACCTGCAGTCTCCACTCGACCCCAGCACCCTGCTGGAGGAAGTCTG
TGTGGAGCAGTGCACCTTCATGGACTCCAAAATGAAGCCCCTGTGGATCATGTACAGCAGCGAGGAGGCG
GGCAGTGCTGGCAACGTGGGCATCATCTTTAAGAACGGGGATGACCTCCGCCAGGACATGCTGACTCTGC
AGATGATCCAGCTCATGGACGTCCTGTGGAAGCAGGAGGGCCTGGACCTGAGGATGACGCCCTACGGCTG
CCTCCCCACCGGGGACCGCACAGGTCTCATCGAGGTGGTCCTCCACTCGGACACCATCGCCAACATCCAG
CTGAACAAAAGCAACATGGCGGCCACAGCTGCCTTCAACAAGGACGCCCTGCTCAACTGGCTCAAGTCCA
AGAACCCTGGGGAGGCCCTGGATCGGGCCATTGAGGAATTCACCCTCTCCTGTGCTGGCTACTGTGTGGC
CACATATGTTCTGGGCATCGGTGACCGGCACAGCGACAACATCATGATCAGAGAGAGTGGGCAGCTCTTC
CACATTGATTTTGGCCACTTTCTGGGGAACTTCAAGACCAAGTTTGGAATCAACCGAGAGCGCGTCCCCT
TCATTCTCACCTACGACTTTGTCCACGTGATCCAGCAGGGGAAGACTAACAACAGTGAGAAGTTTGAAAG
GTTCCGCGGCTACTGTGAACGAGCCTATACCATCCTGCGGCGCCACGGGCTGCTTTTCCTCCATCTCTTC
GCCCTGATGCGGGCCGCAGGTCTGCCTGAGCTTAGCTGCTCCAAAGATATCCAGTATCTCAAGGACTCTC
TGGCACTGGGGAAGACGGAGGAAGAGGCGCTAAAGCACTTCCGGGTGAAGTTCAACGAAGCTCTCCGAGA
AAGCTGGAAAACCAAAGTCAACTGGCTGGCGCACAATGTGTCCAAGGATAACCGACAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001164051
Insert Size 3141 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001164051.1, NP_001157523.1
RefSeq Size 4942 bp
RefSeq ORF 3141 bp
Locus ID 18707
Gene Summary Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (6) represents use of an alternate promoter and 5' UTR, and uses two alternate in-frame splice sites in the central coding region, compared to variant 4. The resulting isoform (e) lacks a 1-aa internal segment and includes an additional 3-aa internal segments, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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