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Rag1 (NM_009019) Mouse Untagged Clone
CAT＃: MC223320
Rag1 (untagged) - Mouse recombination activating gene 1 (Rag1), (10ug)
CNY 13,400.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Rag-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223320 representing NM_009019
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCCTTGCCGTCTACCCTGAGCTTCAGTTCTGCACCCGATGAAATTCAACACCCACAAATCA AATTTTCCGAGTGGAAATTTAAGCTGTTTAGGGTGAGATCCTTTGAAAAGGCACCCGAAGAAGCACAGAA GGAGAAGGATTCCTCAGAGGGGAAACCTTACCTAGAACAGTCTCCAGTAGTTCCAGAGAAGCCTGGTGGT CAGAACTCAATTCTGACTCAACGAGCACTGAAACTCCATCCTAAATTTTCAAAGAAATTCCATGCTGATG GGAAGTCAAGCGACAAAGCAGTTCACCAAGCCAGGCTTAGACACTTCTGCCGCATCTGTGGGAATCGTTT CAAGAGTGACGGGCACAGCCGGAGATACCCAGTCCACGGGCCCGTGGACGCTAAAACCCAAAGTCTTTTC CGAAAGAAGGAAAAAAGAGTCACTTCCTGGCCAGACCTCATTGCCAGGATTTTCCGGATCGACGTGAAGG CAGATGTTGACTCCATCCACCCGACGGAATTCTGCCATGACTGTTGGAGCATCATGCACAGAAAGTTCAG CAGTTCCCACAGTCAGGTCTACTTCCCAAGGAAAGTGACCGTGGAGTGGCACCCCCACACACCGTCCTGT GACATCTGTTTTACTGCCCATCGGGGACTCAAGAGGAAGAGACATCAGCCCAATGTGCAGCTCAGCAAGA AACTAAAAACTGTGCTCAACCACGCGAGACGGGACCGTCGCAAGAGAACTCAGGCTAGGGTCAGCAGCAA GGAAGTCCTGAAGAAGATCTCCAACTGCAGTAAGATTCATCTCAGTACCAAGCTTCTTGCCGTGGACTTC CCAGCACACTTTGTGAAATCCATCTCCTGCCAGATATGCGAACACATTCTGGCTGATCCCGTGGAGACCA GCTGCAAGCATCTATTCTGTAGGATCTGCATTCTCAGATGTCTCAAAGTCATGGGCAGCTATTGTCCCTC TTGCCGATATCCGTGCTTCCCTACTGACCTGGAGAGCCCAGTGAAGTCCTTTCTGAACATCTTGAATTCT CTCATGGTCAAGTGTCCCGCGCAAGATTGCAATGAGGAAGTGAGTCTGGAAAAATATAACCACCATGTGT CAAGCCACAAAGAATCTAAAGAGACTTTGGTGCATATCAATAAAGGGGGACGGCCTCGCCAGCATCTCCT GTCACTGACGAGAAGGGCGCAGAAACATCGGCTGAGGGAGCTCAAGATTCAAGTCAAAGAATTTGCTGAC AAAGAAGAAGGTGGAGATGTGAAGGCTGTCTGCTTGACATTGTTTCTCCTGGCACTGAGGGCGAGGAATG AGCACAGGCAAGCTGATGAATTAGAGGCCATCATGCAAGGCAGGGGCTCCGGGCTTCAACCAGCTGTTTG CTTGGCCATCCGTGTCAATACCTTCCTCAGCTGTAGCCAATACCATAAGATGTACAGGACTGTGAAAGCT ATCACTGGGAGGCAGATTTTTCAACCTTTGCATGCTCTTCGGAATGCCGAGAAAGTCCTTCTGCCAGGCT ACCATCCCTTTGAGTGGCAGCCCCCACTGAAGAATGTGTCCTCCAGAACTGATGTTGGAATTATTGATGG GCTGTCTGGACTTGCCTCCTCTGTGGATGAGTACCCAGTAGATACCATTGCGAAGAGGTTCCGCTACGAC TCTGCTTTGGTGTCTGCTTTGATGGACATGGAAGAAGACATCTTGGAAGGCATGAGATCCCAAGATCTTG ATGACTACCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGA GAAGCACGGGAGTGGGCCCGCAGTTCCAGAAAAGGCCGTTCGTTTCTCTTTCACAGTCATGAGAATTACG ATAGAGCATGGTTCACAGAACGTGAAGGTGTTTGAGGAACCCAAGCCCAATTCTGAACTGTGTTGCAAGC CGTTGTGTCTTATGCTGGCAGATGAGTCTGACCATGAGACCCTTACTGCTATTCTAAGCCCCCTCATTGC CGAGAGGGAGGCCATGAAGAGCAGTGAATTAACGCTGGAGATGGGAGGCATCCCCAGGACTTTTAAATTC ATCTTCAGGGGCACTGGATACGATGAAAAACTTGTCCGGGAAGTAGAAGGCTTGGAAGCTTCTGGCTCAG TCTACATCTGTACACTCTGTGACACCACCCGTTTGGAAGCCTCTCAGAATCTTGTCTTCCACTCCATAAC CAGAAGCCACGCCGAGAACCTGCAGCGCTATGAGGTCTGGCGGTCCAATCCGTATCATGAGTCCGTGGAA GAGCTCCGGGACCGGGTGAAAGGGGTCTCTGCCAAACCTTTCATCGAGACAGTCCCTTCCATAGATGCGC TTCACTGTGACATTGGCAATGCAGCTGAATTCTATAAGATTTTCCAGCTGGAGATAGGGGAAGTGTATAA ACATCCCAATGCCTCTAAAGAGGAAAGGAAGAGATGGCAGGCCACGCTGGACAAACATCTCCGGAAAAGG ATGAACTTAAAACCAATCATGAGGATGAATGGCAACTTTGCCCGGAAGCTTATGACCCAAGAGACTGTAG ACGCAGTTTGTGAGTTAATTCCTTCTGAGGAGAGGCATGAAGCTCTCAGGGAGCTCATGGACCTTTACCT GAAGATGAAACCCGTGTGGCGCTCTTCATGTCCCGCTAAAGAGTGTCCAGAGTCCCTCTGTCAGTACAGT TTCAACTCACAGCGTTTCGCGGAACTCCTCTCCACCAAGTTCAAATATAGATACGAGGGCAAAATCACCA ATTACTTTCACAAAACCTTGGCACATGTCCCTGAAATTATTGAAAGGGATGGCTCTATCGGGGCCTGGGC AAGTGAGGGAAATGAATCGGGTAACAAGCTGTTTAGACGGTTTCGGAAAATGAATGCCAGGCAGTCCAAG TGCTATGAGATGGAAGATGTCCTGAAACATCACTGGCTGTATACTTCAAAATACCTCCAGAAGTTTATGA ATGCTCATAACGCGTTAAAAAGCTCTGGGTTTACCATGAACTCAAAGGAGACCTTAGGGGACCCTTTGGG CATTGAGGACTCTCTGGAAAGCCAAGATTCAATGGAGTTTTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_009019 |
| Insert Size | 3123 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009019.2, NP_033045.2 |
| RefSeq Size | 6669 bp |
| RefSeq ORF | 3123 bp |
| Locus ID | 19373 |
| UniProt ID | P15919 |
| Gene Summary | Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Physiological significance of recombination-activating gene 1 in neuronal death, especially optic neuropathy.
,null,
The FEBS journal
,PubMed ID 25312244
[Rag1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227208 | Rag1 (tGFP-tagged) - Mouse recombination activating gene 1 (Rag1), (10ug) |
CNY 9,220.00 |
|
| MR227208 | Rag1 (Myc-DDK-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 7,240.00 |
|
| MR227208L3 | Lenti ORF clone of Rag1 (Myc-DDK-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 10,260.00 |
|
| MR227208L4 | Lenti ORF clone of Rag1 (mGFP-tagged) - Mouse recombination activating gene 1 (Rag1) |
CNY 10,260.00 |
