Itga4 (NM_010576) Mouse Untagged Clone

CAT#: MC223264

Itga4 (untagged) - Mouse integrin alpha 4 (Itga4), (10ug)



  "NM_010576" in other vectors (6)

CNY 13,300.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CD49D; Itga4B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223264 representing NM_010576
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGAAGCGAGGTGCAGACCGAGGTCCCGAGGGATCGCCCTCCGGGAAGCGGTGATGCTGTTGT
TGTACTTCGGGGTGCCAACCGGGCACTCCTACAACCTGGACCCGGAGAATGCACTGCTGTACCAGGGCCC
CTCCGGCACGCTGTTTGGCTACTCGGTGGTGCTGCACAGCCACGGGTCGAAGCGCTGGCTCATCGTGGGG
GCTCCCACTGCCAGCTGGCTCTCTAATGCCTCAGTGGTCAATCCTGGGGCGATTTACAGATGCGGGATCA
GAAAGAATCCAAACCAGACCTGCGAACAGCTCCAGCTGGGTAGCCCCAGTGGAGAGCCTTGTGGGAAGAC
ATGCCTGGAGGAGAGGGATAACCAGTGGCTGGGGGTCACCCTTTCCAGACAGCCTGGAGAAAATGGCTCT
ATCGTGACTTGTGGGCACAGGTGGAAAAATATTTTTTACATGAAGAGCGATAACAAACTCCCCACTGGCA
TTTGCTACGTCATGCCTTCTGATTTGCGGACAGAACTGAGTAAAAGGATGGCCCCGTGTTACAAAGATTA
TACGAGAAAATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCTAGTTTTTACACACAGGATTTA
ATTGTGATGGGGGCCCCGGGATCATCGTACTGGACTGGCACCGTCTTTGTCTACAATATAACTACAAACC
AATACAAAGCATTTGTAGACAGACAGAACCAAGTAAAATTTGGAAGCTACTTAGGCTACTCAGTTGGAGC
TGGACATTTTCGAAGTCCACATACTACCGAAGTCGTGGGAGGAGCCCCTCAACACGAACAGATAGGAAAA
GCATATATATTTAGCATTGATGAAAACGAACTGAACATCGTATATGAAATGAAAGGTAAAAAGCTTGGCT
CATACTTTGGAGCTTCTGTCTGCGCTGTGGACCTCAATGCAGATGGCTTCTCAGATCTCCTTGTTGGAGC
TCCCATGCAGAGCACCATCAGGGAGGAAGGAAGAGTATTCGTGTACATCAACTCTGGCATGGGAGCTGTG
ATGGTTGAAATGGAAAGGGTCCTTGTCGGAAGTGACAAATATGCTGCAAGATTTGGGGAGTCTATAGCGA
ATCTTGGCGACATTGACAATGACGGCTTTGAAGATATTGCTATTGGTGCACCACAAGAAGACGACTTGAG
AGGTGCTGTCTACATTTACAATGGCCGAGTCGATGGAATCTCCTCCACCTACTCACAGAGAATTGAAGGA
CAGCAAATCAGCAAATCATTAAGGATGTTTGGACAATCTATCTCAGGACAAATTGATGCAGACAACAATG
GATATGTTGATGTAGCCGTTGGTGCATTTCAATCTGATTCTGCAGTGTTGCTAAGGACAAGGCCTGTAGT
GATTGTTGAAGCATCTTTAAGCCATCCTGAGTCTGTAAATAGGACAAAGTTTGACTGTACTGAAAATGGA
CTTCCATCTGTGTGCATGCATCTTACACTGTGTTTCTCATATAAAGGCAAAGAGGTCCCAGGCTACATCG
TTTTGTTTTACAATGTGAGCTTGGATGTGCACAGGAAGGCAGAGTCTCCGTCAAGATTTTATTTCTTCTC
TAATGGGACTTCTGACGTGATTACAGGAAGCATACGAGTTTCAAGCAGTGGAGAGAAATGTAGGACACAC
CAGGCATTCATGCGGAAAGACGTGCGAGACATCCTTACCCCCATTCATGTAGAGGCCACATACCACCTTG
GGCATCATGTGATCACCAAACGAAACACTGAGGAATTTCCACCACTCCAGCCGATCCTTCAGCAGAAGAA
AGAAAAAGACGTTATTAGAAAAATGATAAACTTTGCAAGGTTTTGTGCCTATGAAAATTGCTCTGCTGAT
CTCCAAGTTTCTGCAAAAGTTGGATTTTTGAAGCCATATGAAAATAAAACCTATCTTGCTGTTGGGAGCA
TGAAGACCATAATGCTAAACGTGTCCTTGTTCAACGCTGGCGATGATGCTTACGAAACCACTCTGAATGT
CCAACTCCCCACAGGCCTTTATTTCATTAAGATCTTAGACCTGGAAGAGAAACAAATAAACTGCGAAGTG
ACTGAGAGCTCAGGCATAGTGAAGCTTGCCTGCAGCCTAGGTTACATATATGTGGATCGCCTCTCAAGGA
TAGACATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGGGCACATGAGGACCTCAGCATCAGTGTGCA
TGCCTCCTGTGAAAACGAGGGCGAATTGGACCAAGTGAGGGACAACAGAGTGACCTTAACGATACCTCTA
AGGTATGAGGTTATGCTGACTGTTCATGGGCTTGTGAACCCAACTTCATTTGTGTATGGATCTAGCGAAG
AAAACGAGCCAGAAACATGCATGGCCGAGAAGCTGAACCTCACTTTCCATGTTATAAACACTGGGATTAG
CATGGCTCCAAATGTTAGTGTGAAAATAATGGTACCAAATTCTTTTCTCCCTCAAGATGATAAGTTGTTC
AACGTTTTGGATGTCCAGACAACTACAGGGCAATGCCATTTTAAACACTATGGAAGAGAGTGTACATTTG
CACAGCAAAAAGGCATAGCGGGGACGTTGACCGATATAGTCAAATTCCTATCAAAGACTGATAAGAGACT
CCTGTATTGCATGAAAGCTGATCAACACTGTTTAGATTTCTTATGCAATTTCGGAAAAATGGAAAGTGGG
AAGGAAGCCAGCGTTCATATTCAGCTGGAGGGCAGGCCATCCATCTTGGAAATGGATGAGACCTCATCAC
TCAAGTTTGAAATAAAAGCAACAGCTTTTCCAGAGCCACACCCAAAAGTTATTGAACTAAATAAAGATGA
GAACGTGGCCCATGTTTTCTTGGAAGGGCTCCATCATCAAAGACCCAAACGACATTTCACCATCATTATT
ATTACCATCAGCTTGCTACTTGGACTTATTGTACTTTTATTAATTTCATGTGTTATGTGGAAGGCTGGAT
TCTTTAAAAGACAGTACAAATCTATCCTACAAGAAGAAAACAGGAGAGACAGCTGGAGTTATGTCAACAG
CAAAAGCAATGATGACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010576
Insert Size 3099 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010576.3, NP_034706.3
RefSeq Size 9833 bp
RefSeq ORF 3099 bp
Locus ID 16401
UniProt ID Q00651
Gene Summary Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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