CD117 (NM_001122733) Mouse Untagged Clone

CAT#: MC222941

Kit (untagged) - Mouse kit oncogene (Kit), transcript variant 1, (10ug)



  "NM_001122733" in other vectors (4)

CNY 10,768.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
CD117 (c-kit, Receptor for Stem Cell Factor), rat anti mouse, clone ACK4
    • 200 ug

CNY 6,450.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms belly; Bs; c-KI; c-KIT; CD117; Fdc; Gsfs; Gsfsco1; Gsfsco5; Gsfsow3; SC; SCO1; SCO5; SO; SOW3; Ssm; Tr-k; Tr-kit; W
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222941 representing NM_001122733
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGGCGCTCGCGGCGCCTGGGATCTGCTCTGCGTCCTGTTGGTCCTGCTCCGTGGCCAGACAGCCA
CGTCTCAGCCATCTGCAAGTCCAGGGGAGCCGTCTCCGCCATCCATCCATCCAGCACAATCAGAGTTAAT
AGTTGAAGCTGGCGACACCCTCAGCCTGACGTGCATTGATCCCGACTTTGTCAGATGGACTTTCAAGACC
TATTTCAATGAAATGGTTGAGAATAAAAAAAATGAATGGATCCAGGAAAAAGCCGAGGCCACTCGCACGG
GCACATACACGTGCAGCAACAGCAATGGCCTCACGAGTTCTATTTACGTGTTTGTTAGAGATCCTGCCAA
ACTTTTCCTGGTTGGCCTTCCCTTGTTTGGCAAAGAAGACAGCGACGCGCTGGTCCGCTGCCCTCTGACA
GACCCACAGGTGTCCAATTATTCCCTCATCGAGTGTGATGGGAAATCTCTCCCCACGGACCTGACGTTTG
TCCCAAACCCCAAGGCTGGCATCACCATCAAAAACGTGAAGCGCGCCTACCACCGGCTCTGTGTCCGCTG
TGCTGCTCAGCGTGACGGTACATGGCTGCATTCTGACAAATTCACCCTCAAAGTGCGGGCAGCCATCAAG
GCTATCCCTGTTGTGTCTGTGCCTGAAACAAGTCACCTCCTTAAGAAAGGGGACACATTTACGGTGGTGT
GCACCATAAAAGATGTGTCTACATCCGTGAACTCCATGTGGCTAAAGATGAACCCTCAGCCTCAGCACAT
AGCCCAGGTAAAGCACAATAGCTGGCACCGGGGTGACTTCAATTATGAACGCCAGGAGACGCTGACTATC
AGCTCGGCAAGAGTTGACGATTCTGGAGTGTTCATGTGTTATGCCAATAATACTTTTGGATCAGCAAATG
TCACAACAACCTTGAAAGTAGTAGAAAAAGGATTCATCAACATCTCCCCTGTGAAGAACACTACAGTATT
TGTAACCGATGGAGAAAACGTAGATTTGGTTGTTGAATACGAGGCCTACCCCAAACCCGAGCACCAGCAG
TGGATATATATGAACAGGACCTCGGCTAACAAAGGGAAGGATTATGTCAAATCTGATAACAAAAGCAACA
TCAGATATGTGAACCAACTTCGCCTGACCAGATTAAAAGGCACAGAAGGAGGCACTTATACCTTTCTGGT
GTCCAACTCTGATGCCAGTGCTTCCGTGACATTCAACGTTTACGTGAACACAAAACCAGAAATCCTGACG
TACGACAGGCTCATAAATGGCATGCTCCAGTGTGTGGCAGAGGGATTCCCGGAGCCCACAATAGATTGGT
ATTTTTGTACAGGAGCAGAGCAAAGGTGTACCACTCCTGTCTCACCAGTGGACGTACAGGTCCAGAATGT
ATCTGTGTCACCATTTGGAAAACTGGTGGTTCAGAGTTCCATAGACTCCAGCGTCTTCCGGCACAACGGC
ACGGTGGAGTGTAAGGCCTCCAACGATGTGGGCAAGAGTTCCGCCTTCTTTAACTTTGCATTTAAAGGTA
ACAACAAAGAGCAAATCCAGGCCCACACTCTGTTCACGCCGCTGCTCATTGGCTTTGTGGTTGCAGCTGG
CGCGATGGGGATCATTGTGATGGTGCTCACCTACAAATATTTGCAGAAACCCATGTATGAAGTACAATGG
AAGGTTGTCGAGGAGATAAATGGAAACAATTATGTTTACATAGACCCGACGCAACTTCCTTATGATCACA
AATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGAAAGACATTGGGAGCTGGTGCCTTCGGGAAGGTCGT
TGAGGCCACTGCATATGGCTTGATTAAGTCGGATGCTGCCATGACAGTTGCCGTGAAGATGCTCAAACCA
AGTGCCCATTTAACAGAAAGAGAGGCCCTAATGTCGGAACTGAAGGTCCTGAGCTACCTGGGCAATCACA
TGAATATTGTGAACCTGCTTGGCGCATGCACGGTGGGAGGGCCCACCCTGGTCATTACAGAATATTGTTG
CTATGGTGATCTTTTGAATTTTTTGAGAAGGAAGCGTGACTCGTTTATTTTCTCAAAGCAAGAAGAGCAG
GCAGAAGCGGCACTTTATAAGAACCTTCTGCACTCAACGGAGCCTTCCTGTGACAGTTCAAATGAATATA
TGGACATGAAGCCTGGCGTTTCCTACGTGGTGCCAACCAAGACAGACAAGAGGAGATCCGCAAGAATAGA
CTCGTACATAGAAAGAGACGTGACTCCTGCCATCATGGAAGATGACGAGCTGGCTCTGGACCTGGATGAT
TTGCTGAGCTTCTCCTACCAGGTGGCCAAGGGCATGGCGTTCCTCGCCTCCAAGAATTGTATTCACAGAG
ATTTGGCAGCCAGGAATATCCTCCTCACTCACGGGCGGATCACAAAGATTTGCGATTTCGGGCTAGCCAG
AGACATCAGGAATGATTCGAATTACGTGGTCAAAGGAAATGCACGACTGCCCGTGAAGTGGATGGCACCA
GAGAGCATTTTCAGCTGCGTGTACACATTTGAAAGTGATGTCTGGTCCTATGGGATTTTCCTCTGGGAGC
TCTTCTCCTTAGGAAGCAGCCCCTACCCAGGGATGCCGGTCGACTCCAAGTTCTACAAGATGATCAAGGA
AGGCTTCCGGATGGTCAGCCCGGAGCACGCGCCTGCCGAAATGTATGACGTCATGAAGACTTGCTGGGAC
GCTGACCCCTTGAAAAGGCCAACATTCAAGCAGGTTGTCCAACTTATTGAGAAGCAGATCTCGGACAGCA
CCAAGCACATTTACTCCAACTTGGCAAACTGCAACCCCAACCCAGAGAACCCCGTGGTGGTGGACCATTC
CGTGAGGGTCAACTCGGTGGGCAGCAGCGCCTCTTCTACGCAGCCCCTGCTCGTGCACGAAGATGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001122733
Insert Size 2940 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001122733.1, NP_001116205.1
RefSeq Size 5205 bp
RefSeq ORF 2940 bp
Locus ID 16590
UniProt ID P05532
Gene Summary The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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