CD117 Rat Monoclonal Antibody [Clone ID: ACK4]

Specifications

Product Data
Clone Name	ACK4
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.4µg/ml (1:1000) Paraffin sections: does not react on routinely processed paraffin sections. Suggested positive control: Mouse spleen and tongue
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	IL-3 dependent mast cells derived from WB+/+ mice.
Specificity	Mouse: receptor for stem cell factor Other species: not tested Epitope: The antigen is a 145kD membrane protein (reduced or unreduced) that belongs to the immunoglobulin supergene family. The epitope has not been characterized. Distribution: Isolated Cells: The antigen is described to be present on 5-10% of total adult bone marrow cells. Tissue Sections: The antigen is expressed on CD117 positive cells in various tissues e.g. in mouse spleen and tongue. There is a staining on cells in the red pulp of spleen, whereas there are only few cells with strong staining in tongue.
Formulation	Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.09% sodium azide as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	kit oncogene
Database Link	Entrez Gene 16590 Mouse UniProt ID P05532
Background	Monoclonal antibody ACK4 recognizes the receptor for Stem Cell Factor (SCF) / Steel Factor. The cellular function of CD117 is that of a growth factor receptor. The ligand exists in both soluble and mambrane form. The interaction between SCF and c-kit is essential for the development of hematopoietic, gonadal and pigment stem cells. c-kit positive cells are a subset of CD34+ hematopoietic precursor cells, expression of CD117 can be found on 5-10% of total adult bone marrow cells.
Synonyms	C-Kit; CD117; PBT; SCFR
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.