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Epha4 (NM_007936) Mouse Untagged Clone
CAT＃: MC222938
Epha4 (untagged) - Mouse Eph receptor A4 (Epha4), (10ug)
CNY 12,640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2900005C20Rik; AI385584; Cek8; Hek8; rb; Sek; Sek1; Tyro1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222938 representing NM_007936
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGATTTTCTATTTCATCCTCTTTTCGTTTCTCTTTGGAATTTGCGACGCTGTCACCGGTTCTA GGGTATACCCCGCGAATGAAGTTACTTTATTGGATTCCAGATCTGTTCAGGGAGAGCTTGGGTGGATAGC AAGCCCTCTGGAAGGAGGGTGGGAGGAAGTGAGCATTATGGATGAGAAAAATACACCGATCCGAACCTAC CAGGTGTGCAACGTGATGGAAGCCAGCCAGAACAACTGGCTGCGAACTGACTGGATCACCCGCGAAGGGG CGCAGAGGGTGTACATTGAAATTAAGTTCACTCTAAGGGACTGCAACAGTCTTCCGGGCGTCATGGGGAC TTGCAAGGAGACGTTTAACCTCTACTACTATGAGTCGGACAACGACAAGGAGCGCTTCATCAGAGAGAGC CAGTTTGGCAAGATCGACACCATCGCAGCTGATGAGAGCTTCACGCAGGTGGACATTGGTGACCGAATCA TGAAACTCAACACTGAGATCCGGGATGTAGGGCCACTGAGCAAGAAAGGGTTTTACCTGGCTTTTCAGGA TGTGGGTGCTTGCATCGCCCTGGTGTCTGTCCGTGTGTTCTACAAGAAGTGTCCACTCACCGTTCGAAAT CTAGCCCAGTTTCCTGATACCATTACTGGGGCTGATACCTCTTCCCTGGTGGAAGTTCGAGGCTCCTGTG TCAACAACTCAGAAGAGAAGGATGTGCCAAAAATGTACTGTGGGGCAGATGGTGAATGGCTGGTACCCAT TGGCAACTGCCTATGCAACGCTGGGCACGAGGAGCAGAATGGTGAATGCCAAGCTTGCAAAATTGGATAT TACAAAGCTCTTTCCACGGACGCCTCCTGTGCCAAGTGTCCACCGCACAGCTACTCTGTCTGGGAAGGAG CCACGTCCTGCACCTGTGACCGAGGCTTTTTCAGAGCTGACAACGATGCAGCCTCCATGCCCTGCACCCG CCCACCATCTGCTCCCCTCAACTTGATTTCTAATGTCAACGAGACATCGGTGAACTTGGAATGGAGCAGC CCTCAGAACACAGGTGGCCGTCAGGACATTTCTTACAACGTGGTCTGCAAGAAATGTGGAGCTGGTGATC CCAGCAAGTGCCGGCCCTGTGGAAGTGGAGTCCACTACACACCACAGCAGAACGGACTCAAGACGACCAG AGTCTCCATCACTGACCTCCTAGCACACACCAATTACACGTTTGAGATCTGGGCAGTGAATGGAGTGTCT AAGTATAACCCTAGCCCGGACCAGTCCGTGTCTGTCACAGTGACAACCAACCAAGCAGCACCATCATCCA TTGCTTTGGTCCAGGCTAAAGAAGTTACAAGATATAGCGTAGCTCTGGCTTGGCTGGAACCAGATCGACC AAATGGAGTCATCTTAGAATATGAAGTCAAATATTATGAAAAGGATCAGAATGAACGCAGCTATCGCATA GTCCGGACAGCTGCCAGGAACACAGATATCAAAGGCCTGAACCCTCTGACTTCCTATGTTTTTCACGTGC GAGCCAGGACCGCTGCTGGCTACGGAGACTTCAGCGAGCCCCTGGAAGTCACTACTAATACAGTGCCTTC CCGCATCATTGGCGATGGAGCCAACTCCACTGTCCTGCTGGTCTCCGTCTCTGGCAGTGTGGTCCTCGTG GTCATTCTCATTGCAGCTTTTGTGATCAGCCGAAGACGGAGTAAGTACAGCAAAGCGAAACAAGAAGCAG ATGAAGAGAAACATTTGAATCAAGGTGTTAGAACTTATGTGGATCCCTTTACATACGAAGACCCCAACCA GGCAGTTCGAGAATTTGCCAAAGAAATCGATGCATCCTGCATTAAAATTGAAAAGGTCATAGGAGTTGGT GAATTTGGAGAGGTCTGCAGTGGGCGTCTCAAAGTGCCCGGCAAGAGAGAGATCTGTGTGGCCATCAAGA CTCTGAAAGCTGGCTATACTGACAAGCAGAGGAGAGACTTCCTGAGTGAGGCCAGCATCATGGGACAGTT TGACCACCCAAACATAATCCACCTGGAAGGCGTTGTCACCAAATGTAAACCGGTAATGATCATAACGGAG TACATGGAGAACGGCTCCTTGGATGCTTTCCTCAGGAAGAATGATGGCCGCTTTACAGTCATTCAGCTGG TGGGCATGCTCCGGGGCATTGGCTCGGGGATGAAGTACTTATCTGATATGAGCTATGTGCATCGAGACCT GGCTGCCAGGAACATCCTGGTAAACAGCAACTTGGTCTGCAAGGTGTCTGACTTTGGCATGTCCAGGGTG CTGGAGGATGACCCCGAAGCAGCCTACACTACCAGGGGTGGCAAGATTCCTATCCGGTGGACTGCGCCAG AAGCAATTGCGTATCGTAAATTTACCTCAGCCAGTGATGTCTGGAGCTACGGAATCGTTATGTGGGAAGT GATGTCATATGGAGAGAGACCGTACTGGGATATGTCCAATCAAGATGTGATCAAAGCCATCGAGGAAGGG TACCGGCTACCCCCTCCAATGGACTGCCCCATTGCCCTCCATCAGTTAATGCTGGACTGCTGGCAGAAAG AGAGAAGTGACAGGCCTAAATTTGGGCAGATCGTCAACATGCTGGACAAACTCATCCGCAATCCCAACAG CCTGAAGAGGACAGGGTCGGAGAGTTCCAGACCAAACACAGCCTTGTTAGATCCCAGCTCCCCTGAATTC TCTGCTGTAGTATCAGTGGGCGACTGGCTGCAGGCCATCAAAATGGACCGGTATAAGGACAACTTCACGG CCGCCGGGTACACGACACTAGAAGCCGTGGTTCACATGAGCCAGGACGACCTGGCGAGAATTGGCATCAC CGCCATCACGCACCAGAATAAGATTTTGAGCAGCGTCCAGGCGATGCGAACCCAGATGCAGCAGATGCAT GGCAGGATGGTTCCTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007936 |
| Insert Size | 2961 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007936.3, NP_031962.2 |
| RefSeq Size | 6328 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 13838 |
| UniProt ID | Q03137 |
| Gene Summary | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211389 | Epha4 (tGFP-tagged) - Mouse Eph receptor A4 (Epha4) |
CNY 7,890.00 |
|
| MR211389 | Epha4 (Myc-DDK-tagged) - Mouse Eph receptor A4 (Epha4) |
CNY 7,224.00 |
|
| MR211389L3 | Lenti ORF clone of Epha4 (Myc-DDK-tagged) - Mouse Eph receptor A4 (Epha4) |
CNY 9,030.00 |
|
| MR211389L4 | Lenti ORF clone of Epha4 (mGFP-tagged) - Mouse Eph receptor A4 (Epha4) |
CNY 9,030.00 |
