Dspp (NM_010080) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D; Dmp; Dmp2; Dmp3; Dpp; Dsp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222823 representing NM_010080 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGAAGATAATTATATATATATGCATTTGGGCAACTGCCTGGGCCATTCCGGTTCCCCAGTTAG TACCACTGGAAAGAGACATTGTTGAAAACTCTGTGGCTGTGCCTCTTCTAACACATCCAGGAACTGCAGC ACAGAATGAGTTATCTATCAACAGCACCACTAGCAACAGCAACGACTCCCCAGATGGCAGTGAGATAGGA GAGCAGGTACTTAGCGAGGATGGTTACAAAAGAGATGGGAATGGCTCCGAGTCAATACATGTAGGAGGGA AGGATTTTCCTACTCAGCCCATTTTAGTAAACGAACAGGGGAACACTGCTGAAGAACACAATGACATAGA AACATACGGTCATGATGGGGTACATGCGAGAGGAGAGAACAGCACAGCAAATGGCATCAGGAGCCAGGTA GGCATCGTTGAAAATGCGGAGGAAGCAGAGAGCAGTGTCCACGGACAGGCTGGTCAGAATACAAAATCTG GAGGTGCTAGTGATGTAAGCCAGAATGGAGATGCGACCCTTGTCCAGGAAAATGAGCCTCCAGAAGCTAG CATCAAGAATAGCACCAACCATGAGGCTGGAATACACGGGAGTGGGGTTGCTACACATGAAACGACGCCT CAGAGAGAAGGGCTGGGGAGTGAGAACCAGGGGACTGAGGTGACACCAAGCATCGGGGAAGATGCAGGTT TGGATGATACTGATGGGAGTCCTAGCGGGAACGGGGTAGAGGAGGATGAAGATACAGGCTCTGGTGATGG TGAGGGTGCAGAAGCAGGAGATGGAAGGGAGAGCCATGATGGCACTAAGGGCCAGGGGGGCCAATCTCAT GGGGGAAACACTGACCACAGAGGTCAGAGTTCAGTTAGTACTGAAGATGATGATTCTAAAGAACAAGAAG GCTTCCCAAATGGACACAATGGAGACAACAGCAGTGAGGAAAACGGTGTTGAAGAAGGCGACAGTACCCA GGCAACGCAGGACAACCAAAAGCTCAGCCCCAAAGACACCCGAGATGCAGAGGGTGGGATCATCAGCCAG TCAGAAGCATGTCCTTCTGGGAAGAGCCAAGATCAGGGAATAGAAACTGAAGGTCCCAACAAAGGCAACA AAAGTATTATTACCAAAGAATCTGGGAAACTCAGTGGAAGTAAAGATAGCAATGGACACCAAGGAGTGGA GCTGGACAAAAGGAATAGCCCAAAGCAAGGGGAGTCTGACAAGCCTCAAGGCACTGCTGAGAAATCAGCT GCCCACAGTAACCTGGGACACAGCAGGATAGGTAGCAGCAGCAATAGTGATGGGCATGACAGTTACGAGT TCGATGACGAGTCCATGCAAGGAGATGATCCCAAGAGCAGCGACGAATCTAACGGAAGTGACGAAAGTGA CACTAACTCTGAAAGCGCCAATGAGAGTGGCAGCCGTGGAGATGCTTCTTACACATCTGATGAATCAAGT GATGATGACAATGACAGTGACTCACATGCGGGAGAAGACGATAGCAGTGATGACTCATCTGATACAGATG ACAGTGACAGTAATGGTGATGGTGACAGTGACAGTAATGGTGATGGTGACAGCGAGAGTGAGGACAAGGA CGAATCTGACAGCAGTGACCATGACAACAGCAGTGACAGTGAGAGCAAATCAGACAGCAGTGACAGTAGT GACGACAGCAGTGACAGCAGCGACAGTAGTGACAGCAGTGACAGCAGTGACAGTAGTGACAGTAGTGACA GCAGCGACAGCAGTGACAGCAGCGACAGCAACAGTAGTAGTGACAGCAGCGACAGCAGCAGTAGTAGTGA CAGCAGCGACAGCAGTGACAGCAGTGACAGCAGTGACAGCAGCGATAGCAGTGACAGCAGTGACAGCAGT GACAGCAGCGATAGCAGTGACAGCAGTGACAGTAGTGACAGCAGTGACAGCAGCGACAGCAGCAGTAGTA GTGACAGCAGCGACAGCAGCAGTAGTAGTGACAGCAGCGACAGCAGTGACAGCAGTGACAGCAGCGATAG CAGTGACAGCAGTGACAGCAGCAGCAGCGACAGCAGCAGCAGTAGCAACAGCAGTGACAGTAGTGACAGC AGTGACAGCAGCAGCAGCAGCGACAGCAGCAACAGCAGCGACAGCAGTGACAGTAGTGACAGCAGTGACA GTAGTGACAGCAGTGACAGCAGCAACAGTAGTGACAGCAGCGACAGCAGCAGTAGTAGTGACAGCAGCGA CAGCAGCAGTAGTAGTGACAGCAGTGACAGTAGTGACAGTAGTGACAGCAGTGAGAGCAGTGAGAGCAGC GACAGCAGCAACAGCAGTGACAGCAGCGACAGTAGTGACAGCAGTGACAGTAGCGACAGCAGCGACAGTA GTGACAGTAGCGACAGCAGTGACAGTAGCAACAGTAGCGACAGCAGTGACAGCAGTGACAGCAGCGACAG TAGTGACAGCAGCAACAGTAGTGACAGCAGTGACAGTAGCGACAGTAGTGACAGCAGTGACAGCAGTGAC AGCAGCGACAGTAGTGACAGCAGTGACAGTAGTGACAGCAGCGACAGTAGTGACAGCAGTGACAGCAGTG ACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAGCGACAGCAGCGACAGCAGTGACAGCAG CGACAGCAGCAACAGCAGTGACAGCAGTGACAGTGACAGCAAGGATAGCAGTTCTGACAGCAGTGATGGT GACAGCAAGTCTGGTAATGGCAACAGTGACAGCAACAGTGACAGCAACAGTGACAGTGACAGTGACAGTG AAGGCAGTGACAGTAACCACTCAACCAGTGATGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010080
Insert Size	2838 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010080.3, NP_034210.2
RefSeq Size	4440 bp
RefSeq ORF	2838 bp
Locus ID	666279
Gene Summary	This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mice lacking the encoded protein exhibit hypomineralization defects in dentin, similar to human dentinogenesis imperfecta. [provided by RefSeq, Feb 2016]

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