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Ephb1 (NM_001168296) Mouse Untagged Clone
CAT＃: MC222760
Ephb1 (untagged) - Mouse Eph receptor B1 (Ephb1), transcript variant 2, (10ug)
CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9330129L11; AW488255; C130099E04Rik; Cek6; Elk; Elkh; ENSMUSG00000074119; Hek6; Net |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222760 representing NM_001168296
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGATTGCTTGCTGCTCTTCCTCCTGGCATCTGCAGTGGCCGCGATGGAAGAGACATTGATGG ACACAAGGACAGCCACTGCAGAGTTGGGATGGACGGCCAACCCTGCCTCTGGGTGGGAAGAAGTCAGTGG CTATGATGAAAACCTGAACACCATCCGTACTTACCAAGTGTGCAACGTCTTCGAACCCAACCAGAACAAC TGGCTGCTTACCACCTTTATCAACAGAAGGGGCGCCCATCGCATCTATACAGAGATGCGCTTCACTGTGA GGGACTGCAGCAGCCTTCCAAATGTCCCAGGCTCCTGCAAGGAGACCTTCAACTTGTACTACTATGAGAC TGACTCTGTGATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAAGCCCCCTACCTCAAAGTGGACACCATT GCTGCAGATGAGAGCTTCTCCCAGGTGGATTTTGGGGGAAGGTTGATGAAAGTCAACACGGAAGTCAGGA GCTTTGGGCCTCTTACTAGGAACGGTTTTTACCTCGCTTTCCAGGATTATGGAGCCTGTATGTCTCTCCT TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATCGTGCAGAATTTTGCAGTGTTCCCAGAAACCATG ACAGGAGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCAAATGCGGAAGAAGTGGATG TGCCCATAAAACTCTACTGCAACGGAGATGGAGAGTGGATGGTGCCCATTGGGCGCTGTACCTGTAAGCC TGGCTATGAGCCTGAGAACAGCGTGGCCTGCAAGGCCTGTCCTGCGGGGACCTTCAAGGCCAGCCAGGAA GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGTCGCTCCCCTTCAGAGGCGTCTCCCATCTGCACCTGCC GGACTGGCTATTACCGAGCTGACTTTGATCCACCAGAGGTGGCGTGTACTAGTGTCCCATCGGGTCCTCG AAATGTCATCTCCATCGTGAATGAGACATCTATCATTCTAGAGTGGCACCCTCCAAGAGAGACTGGTGGG AGAGATGACGTGACGTACAACATCATCTGCAAGAAGTGCCGAGCAGACCGCCGCAGCTGCTCCCGCTGCG ATGACAATGTGGAGTTTGTCCCCAGGCAACTGGGCTTGACTGAGTGTCGTGTCTCCATCAGTAGCCTATG GGCCCACACCCCGTACACCTTTGATATCCAGGCCATCAATGGAGTCTCTAGCAAGAGTCCCTTTCCCCCA CAGCACGTCTCTGTCAACATCACCACAAACCAAGCTGCCCCCTCCACTGTTCCTATCATGCACCAGGTCA GTGCCACCATGAGGAGCATCACCTTGTCATGGCCTCAGCCGGAGCAACCCAATGGCATAATCCTGGACTA TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCTTCCATGGCCAGGAGCCAGACCAACACA GCACGTATCGATGGGCTACGGCCTGGCATGGTATACGTGGTCCAGGTGCGAGCTCGAACCGTGGCTGGCT ATGGCAAGTTCAGTGGCAAGATGTGTTTCCAGACTCTGACAGATGATGATTACAAGTCGGAGCTGAGAGA GCAGCTACCCCTGATTGCTGGCTCGGCAGCAGCTGGAGTCGTATTTGTTGTGTCTCTGGTGGCCATCTCT ATTGTCTGCAGCAGGAAACGAGCTTACAGCAAAGAGGCTGCGTACAGTGATAAGCTTCAACATTACAGCA CAGGCCGAGGGGAGTTCGGCGAGGTGTACAAGGGCCGTTTGAAGCTGCCAGGCAAGAGGGAAATCTATGT GGCCATCAAGACCCTGAAGGCTGGGTACTCAGAGAAACAGCGTCGGGATTTTCTGAGCGAGGCGAGCATC ATGGGCCAGTTTGACCATCCCAACATCATTCGCCTGGAGGGTGTCGTCACCAAGAGCCGGCCTGTCATGA TCATTACGGAGTTCATGGAGAACGGCGCTTTAGACTCTTTCCTCCGGCAAAATGATGGACAGTTCACCGT GATCCAGCTTGTGGGGATGCTGAGGGGCATCGCTGCTGGCATGAAGTACCTATCTGAGATGAATTATGTG CACCGGGACCTGGCTGCTAGAAACATTCTGGTCAACAGCAACCTGGTGTGCAAAGTTTCTGACTTTGGTC TCTCTCGCTACCTCCAGGATGACACCTCAGACCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGT GAGATGGACAGCTCCAGAGGCCATCGCCTACCGCAAGTTTACGTCAGCCAGCGATGTCTGGAGCTATGGG ATTGTCATGTGGGAAGTGATGTCATTTGGAGAGAGACCTTACTGGGATATGTCCAATCAAGATGTCATCA ATGCCATTGAGCAGGATTACCGGCTGCCTCCTCCTATGGACTGCCCAGCTGCCCTGCACCAGCTCATGCT GGACTGTTGGCAGAAGGATCGCAATAGCCGGCCCCGTTTTGCAGAGATCGTCAACACCCTGGACAAGATG ATCCGGAACCCAGCTAGTCTCAAGACTGTGGCAACCATCACCGCTGTGCCTTCCCAACCCCTGCTTGACC GCTCTATCCCAGACTTCACGGCCTTTACCACCGTGGATGACTGGCTAAGTGCCATCAAAATGGTCCAGTA CAGGGACAGCTTCCTCACCGCAGGCTTCACCTCCCTTCAGCTGGTCACCCAGATGACATCAGAAGACCTC CTGAGAATAGGGGTAACCTTGGCAGGCCATCAGAAGAAGATTCTGAGTAGCATTCACTCAATGAGGGTCC AGATGAACCAGTCACCATCGGTAATGGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001168296 |
| Insert Size | 2832 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001168296.1, NP_001161768.1 |
| RefSeq Size | 4563 bp |
| RefSeq ORF | 2832 bp |
| Locus ID | 270190 |
| UniProt ID | Q8CBF3 |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (PubMed:27446912).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a coding exon compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226017 | Ephb1 (tGFP-tagged) - Mouse Eph receptor B1 (Ephb1) transcript variant 2, (10ug) |
CNY 9,120.00 |
|
| MR226017 | Ephb1 (Myc-DDK-tagged) - Mouse Eph receptor B1 (Ephb1), transcript variant 2 |
CNY 6,904.00 |
|
| MR226017L3 | Lenti ORF clone of Ephb1 (Myc-DDK-tagged) - Mouse Eph receptor B1 (Ephb1), transcript variant 2 |
CNY 10,170.00 |
|
| MR226017L4 | Lenti ORF clone of Ephb1 (mGFP-tagged) - Mouse Eph receptor B1 (Ephb1), transcript variant 2 |
CNY 10,170.00 |
