Adamts5 (NM_011782) Mouse Untagged Clone

CAT#: MC222688

Adamts5 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), (10ug)



  "NM_011782" in other vectors (4)

CNY 10,224.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9530092O11Rik; ADAM-; ADAM-TS5; ADAMTS1; ADAMTS11; ADMP-2; AI481094; ASMP-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222688 representing NM_011782
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCGAGTGGGCGCCCTTGTTGCTGCTACTGCTGCTGCTGAGCGCGTCCTGCCTGTCCCTGGCCG
CTGACAGCCCCGCCGCGGCACCTGCCCAGGATAAAACCAGGCAGCCTCAGGCTGCAGCAGCGGCCGCCGA
GCCGGACCAGCCGCAGGGGGAGGAAACACGGGAGCGAGGCCATTTACAACCCTTGGCCGGGCAGCGCAGG
AGCGGCGGGCTGGTCCAGAATATAGACCAACTCTACTCTGGCGGTGGCAAAGTGGGCTACCTTGTCTACG
CGGGCGGCCGGAGGTTCCTGCTGGACCTGGAGAGAGATGACACAGTGGGTGCTGCTGGTAGCATCGTTAC
TGCAGGAGGAGGGCTGAGCGCATCCTCTGGCCACCGGGGTCACTGTTTCTACAGAGGCACCGTGGACGGC
AGCCCTCGATCCCTAGCTGTCTTTGACCTCTGCGGGGGTCTCGATGGCTTCTTTGCAGTCAAGCATGCGC
GCTACACTCTAAAGCCACTCCTGCGTGGGTCCTGGGCAGAGTATGAACGAATTTATGGGGATGGATCTTC
CCGCATCCTGCATGTCTACAACCGCGAGGGCTTTAGCTTCGAGGCCCTGCCGCCACGCGCCAGTTGCGAG
ACTCCTGCATCCCCATCTGGGCCCCAAGAGAGCCCCTCGGTGCACAGTAGATCTAGGAGACGCTCAGCGC
TGGCCCCGCAGCTGCTGGACCACTCAGCTTTCTCGCCATCTGGGAACGCGGGACCTCAGACTTGGTGGAG
GCGTAGGCGCCGTTCCATCTCCAGGGCCCGCCAGGTGGAGCTCCTCTTGGTGGCTGACTCGTCCATGGCC
AGGATGTATGGGCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAACAGGCTGTACAGTC
ATGCAAGCATTGAGAACCACATCCGCCTGGCGGTGGTGAAGGTGGTGGTGCTGACGGACAAGGACACGAG
TCTGGAGGTGAGCAAGAATGCGGCCACGACCCTCAAGAACTTTTGCAAATGGCAGCACCAACATAACCAG
CTAGGGGATGATCACGAAGAGCACTACGATGCAGCCATCCTGTTCACCCGAGAGGATTTATGTGGGCATC
ATTCATGTGACACCCTGGGAATGGCAGACGTTGGGACCATATGTTCTCCGGAGCGCAGCTGTGCAGTGAT
TGAAGATGATGGCCTCCATGCAGCCTTCACTGTGGCTCATGAAATTGGGCATCTACTTGGCCTTTCTCAT
GACGATTCCAAATTCTGTGAAGAGAACTTCGGTACTACAGAAGACAAGCGTTTAATGTCTTCAATCCTTA
CCAGCATCGATGCATCCAAGCCCTGGTCCAAATGCACGTCAGCCACCATCACAGAATTCCTGGATGATGG
TCATGGTAATTGTTTGCTAGACCTACCACGGAAGCAGATTTTGGGTCCCGAGGAACTCCCAGGACAGACC
TACGATGCCACCCAGCAGTGCAACTTGACATTTGGGCCTGAGTACTCGGTGTGCCCTGGCATGGATGTCT
GTGCGCGGCTGTGGTGTGCTGTGGTGCGCCAAGGCCAAATGGTGTGTCTGACCAAGAAGCTGCCGGCTGT
GGAGGGCACTCCCTGTGGGAAGGGAAGAGTCTGCCTTCAAGGCAAATGTGTGGACAAAACTAAGAAAAAA
TATTACTCGACATCAAGCCATGGAAATTGGGGGTCCTGGGGCCCCTGGGGTCAGTGTTCTCGCTCATGCG
GGGGAGGAGTGCAGTTTGCCTACCGCCATTGTAATAACCCTGCACCTCGAAACAGTGGCCGCTACTGCAC
AGGGAAGAGGGCCATATACCGTTCCTGCAGTGTTACACCCTGCCCACCCAATGGTAAATCTTTTCGCCAT
GAGCAGTGTGAAGCCAAAAATGGCTATCAGTCTGATGCAAAAGGAGTCAAAACATTTGTAGAATGGGTTC
CCAAATATGCAGGTGTCCTGCCGGCAGATGTGTGCAAGCTTACCTGCAGAGCTAAGGGCACAGGCTACTA
TGTGGTCTTTTCTCCAAAGGTTACGGATGGGACTGAATGCAGGCCGTACAGCAACTCTGTGTGTGTCCGA
GGACGGTGTGTGAGAACTGGATGTGACGGCATTATTGGCTCAAAGCTACAATATGACAAGTGTGGAGTGT
GCGGAGGGGATAACTCCAGTTGTACAAAGATTATCGGAACCTTCAATAAAAAAAGCAAGGGTTATACTGA
CGTTGTGAGGATCCCTGAAGGAGCAACCCACATAAAAGTCCGACAGTTCAAAGCCAAAGACCAGACTAGA
TTCACTGCCTACTTAGCCCTGAAGAAGAAAACTGGCGAGTACCTTATCAATGGCAAGTACATGATTTCCA
CTTCAGAGACCATCATCGACATCAATGGTACCGTCATGAACTACAGTGGATGGAGCCACAGAGATGATTT
TTTACATGGGATGGGCTATTCAGCCACAAAAGAAATCCTGATCGTGCAGATCCTTGCCACAGACCCAACT
AAAGCGCTAGACGTCCGTTACAGCTTTTTTGTTCCCAAGAAGACCACTCAAAAAGTAAACTCTGTCATCA
GCCATGGCAGCAACAAGGTGGGACCACACTCTACACAGCTGCAGTGGGTGACAGGTCCATGGCTGGCCTG
CTCCAGGACCTGTGACACAGGCTGGCACACTAGGACCGTGCAGTGCCAGGATGGAAACAGGAAATTAGCT
AAAGGATGCCTTCTCTCTCAGAGGCCTTCTGCATTTAAGCAATGTCTGCTGAAGAAATGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_011782
Insert Size 2793 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011782.2, NP_035912.2
RefSeq Size 8104 bp
RefSeq ORF 2793 bp
Locus ID 23794
UniProt ID Q9R001
Gene Summary This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. Mice lacking the encoded protein are protected from surgery-induced osteoarthritis and antigen-induced arthritis. [provided by RefSeq, May 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...