Chaf1a (NM_013733) Mouse Untagged Clone

CAT#: MC222524

Chaf1a (untagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a), (10ug)



  "NM_013733" in other vectors (4)

CNY 11,690.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AL023013; AL024058; Cac1p; CAF-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222524 representing NM_013733
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGAGGAGCCGGAGGCCGCGACGCGGACAGCCGCGGCCGTGGATTGCAAAGACAGACCCGGGTTCC
CAGTGAAGAGGTTAATACAAGCCCGCTTGCCCTTCAAACGCCTGAACCTTGTTCCCAAGGAGAAAGTTGA
GGAAGACACATCACCAAAAGCAGCTGTGGAAAGCAAAGTCCCGGATCTACAATTGTCTTTGGGCACTTTT
GAAAGCCAGTGTCACACAGGCTCTCACGTAGGCTTGAGTACAAAGTTGGTCGGTGGCCAGGGCCCCATTG
ATAGCTTCTTGAGGGCAACAATCAAGCCAGTACCGAGTGTGGTCATTATCGACCTGACGGAGAACTGCAG
TGACATTCCAGACAGCCCTGAGGGCCACAGTGAACTAAGTCCTGACACTGCTGGTGTGGTCACCACAGTG
GAAGGAGCTGCCAAGCAGCAGGAGCACTCTGCAGCAGAGCTGTGTCTCCTAGAGACCCCTTCAGACATCA
CTTGTCACATGGAAGAGGAGCCTGGGAGTCCAGGAGACCCAAAGAGGACGGGTGACTGTCAGGCTGGTTC
ACTACAGAGCTGTCCGGAGCTGACCCCAGGTTCTAGGACATGCCCCACCAAGGAGTTATCAAGCTGGAGC
AAGGCAGGGGATCTCCTATTTATAGAGAAAGTGCCTGTGGTGGTGCTAGAGGACATCCTGGCCACTAAGC
CCTCCATTGCCTCTCTTCCCATGATGTCCCTGGATAGGAGTGTCACTTCAGAGAGTGAAATACTGGAGTC
CTGCCCCGAGGATGACTCCATACTGAGCCACTCCTCCACCAACTCCTCTTCTCCAACCAGCTCTCCTGAG
GGACCATCCACACCCCCAGAACATCGGGGTGGGAGAAGCTCCCCCTCCACCCCAGCCTGCAGAGTTGCCA
AGAACTTTGTCAAAGGCTCCACAGAGAAGGGCAGGAGCAAACTGCACAGAGATAGAGAACAGCAGAGGGA
AGAGAAGGAAAAGCTGAGGGAAGAGATACGACGAGCCAAGGAGGAGGCACGGAAGAAGAAGGAGGAGGAG
AAGGAGCTGAAGGAAAAGGAGCGGAGGGAAAAGCGGGAGAAAGATGAGAAGGAGAAGGCGGAGAAGCAGC
GGCTAAAGGAGGAAAAACGCAAGGAGCGGCAGGAAGCTCTGGAGGCTAAACTGGAGGAGAAGAGGAAGAA
GGAGGAAGAGAAGCGGTTACGGGAGGAAGAGAAGCGGTTACGGGAAGAAGAGAAGCGCATTAAGGCAGAA
AAGGCAGAGATCACTCGATTCTTCCAGAAACCGAAGACTCCGCAGGCTCCCAAGACCCTGGCCGGTTCCT
GTGGTAAGTTTGCCCCCTTTGAAATCAAAGAACACATGGTGCTGGCTCCACGGTGCCGAGCTGCCTTGGA
TCAAGACCTGTGCGATCAGCTGGACCAGCTCCTGCAGCAGCAGAGTGTGGCAAGCACCTTCCTCAGTGAC
CTAAAAAGCCGGCTGCCCTTGAGATCGGGGCCCACCCGGGTCTGTGGCCATGACACAGACATCATGAACA
GGGATGTGGTCATTGTGGAGAGCAGCAAGGTGGACGGTGTGTCTGAAAGGAAGAAGTTTGGCCGCATGAA
GCTTCTGCAGTTCTCAGAGAACCACCGGCCAGCATACTGGGGGACGTGGAACAAGAAGACAGCCATCATC
CGCCCCCGGAACCCCTGGGCCCAGGACAAAGACCTTCTTGACTATGAGGTAGACAGTGACGATGAGTGGG
AAGAGGAGGAACCTGGGGAGTCTTTGTCCCACAGTGAGGGGGATGAGGATGACGATGTGGGGGAAGATGA
GGATGAGGACGATGGCTTCTTTGTGCCCCATGGGTACTTATCTGAAGATGAAGGCGTGACTGAGGAGTGT
GCAGACCCTGAGAACCACAAAGTACACCAGAAGCTGAAGGCCAAGGAGTGGGATGAGCTCCTAGCCAAGG
GCAAGCGCTTTCGTGTGCTGCAGCCTGTGCACGTGGGATGTGTATGGGCATCAGAGGCAGCCAACTGCAC
GAGTTCCGACTTGAAGCTGCTGCAGCAGTTCACTGCATGCCTGCTGGATGTGGCATCCCCTGATGAGCCA
GAACCCGGGGCCTCCAGGAGGGAAAAGAGGGACCAGCATATCCTGGCCCAGTTGCTTCCGCTGCTACATG
GCAATGTGAATGGGAGCAAGGTGATCATCCATGAATTCCAGGAGCAATGCCGCAGGGGCCTGCTGACCCT
TCCAAGTCCCACCCCACACCTGCAGATGCCCAACTTGGAGGATGCTGTTGCTGTGCCATCCAAGGCCAGG
CTGAAGCGCCTTATCTCTGAGAACTCTGCCTATGAGAAGCGGCCCAACTTCCGTATGTGCTGGTATGTGC
ACCCAGAGGTGCTCAAGAGCTTTGGCCAGGAGTGCCTGCCAGTGCCCTGCCAGTGGACCTACATCACCAC
CATGCCCTCAGCACCCAGGGAAGACAGTGGCAGTGCTTCTACTGAGGGGCCTGGCCAGAGCACACCTATG
CTACTCAAGCGGAAGCCAGCGGCCACCATGTGCATCACGCAGTTTATGAAGAAGCGGCGCTATGATGGAC
AGGTTGGGTCTGGGGACATGGATGGCTTCCAGGCAGACACAGAAGAGGATGAAGAGGATGACACAGACTG
TATGATCATAGATGTGCCAGATGTTGGGAGTGACGTGTCAGAAGCTCCCATACCTGCTCCCACACTTTGC
AAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_013733
Insert Size 2736 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013733.3, NP_038761.1
RefSeq Size 3308 bp
RefSeq ORF 2736 bp
Locus ID 27221
UniProt ID Q9QWF0
Gene Summary Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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