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Chaf1a (NM_013733) Mouse Untagged Clone
CAT＃: MC222524
Chaf1a (untagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a), (10ug)
CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AL023013; AL024058; Cac1p; CAF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222524 representing NM_013733
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGAGGAGCCGGAGGCCGCGACGCGGACAGCCGCGGCCGTGGATTGCAAAGACAGACCCGGGTTCC CAGTGAAGAGGTTAATACAAGCCCGCTTGCCCTTCAAACGCCTGAACCTTGTTCCCAAGGAGAAAGTTGA GGAAGACACATCACCAAAAGCAGCTGTGGAAAGCAAAGTCCCGGATCTACAATTGTCTTTGGGCACTTTT GAAAGCCAGTGTCACACAGGCTCTCACGTAGGCTTGAGTACAAAGTTGGTCGGTGGCCAGGGCCCCATTG ATAGCTTCTTGAGGGCAACAATCAAGCCAGTACCGAGTGTGGTCATTATCGACCTGACGGAGAACTGCAG TGACATTCCAGACAGCCCTGAGGGCCACAGTGAACTAAGTCCTGACACTGCTGGTGTGGTCACCACAGTG GAAGGAGCTGCCAAGCAGCAGGAGCACTCTGCAGCAGAGCTGTGTCTCCTAGAGACCCCTTCAGACATCA CTTGTCACATGGAAGAGGAGCCTGGGAGTCCAGGAGACCCAAAGAGGACGGGTGACTGTCAGGCTGGTTC ACTACAGAGCTGTCCGGAGCTGACCCCAGGTTCTAGGACATGCCCCACCAAGGAGTTATCAAGCTGGAGC AAGGCAGGGGATCTCCTATTTATAGAGAAAGTGCCTGTGGTGGTGCTAGAGGACATCCTGGCCACTAAGC CCTCCATTGCCTCTCTTCCCATGATGTCCCTGGATAGGAGTGTCACTTCAGAGAGTGAAATACTGGAGTC CTGCCCCGAGGATGACTCCATACTGAGCCACTCCTCCACCAACTCCTCTTCTCCAACCAGCTCTCCTGAG GGACCATCCACACCCCCAGAACATCGGGGTGGGAGAAGCTCCCCCTCCACCCCAGCCTGCAGAGTTGCCA AGAACTTTGTCAAAGGCTCCACAGAGAAGGGCAGGAGCAAACTGCACAGAGATAGAGAACAGCAGAGGGA AGAGAAGGAAAAGCTGAGGGAAGAGATACGACGAGCCAAGGAGGAGGCACGGAAGAAGAAGGAGGAGGAG AAGGAGCTGAAGGAAAAGGAGCGGAGGGAAAAGCGGGAGAAAGATGAGAAGGAGAAGGCGGAGAAGCAGC GGCTAAAGGAGGAAAAACGCAAGGAGCGGCAGGAAGCTCTGGAGGCTAAACTGGAGGAGAAGAGGAAGAA GGAGGAAGAGAAGCGGTTACGGGAGGAAGAGAAGCGGTTACGGGAAGAAGAGAAGCGCATTAAGGCAGAA AAGGCAGAGATCACTCGATTCTTCCAGAAACCGAAGACTCCGCAGGCTCCCAAGACCCTGGCCGGTTCCT GTGGTAAGTTTGCCCCCTTTGAAATCAAAGAACACATGGTGCTGGCTCCACGGTGCCGAGCTGCCTTGGA TCAAGACCTGTGCGATCAGCTGGACCAGCTCCTGCAGCAGCAGAGTGTGGCAAGCACCTTCCTCAGTGAC CTAAAAAGCCGGCTGCCCTTGAGATCGGGGCCCACCCGGGTCTGTGGCCATGACACAGACATCATGAACA GGGATGTGGTCATTGTGGAGAGCAGCAAGGTGGACGGTGTGTCTGAAAGGAAGAAGTTTGGCCGCATGAA GCTTCTGCAGTTCTCAGAGAACCACCGGCCAGCATACTGGGGGACGTGGAACAAGAAGACAGCCATCATC CGCCCCCGGAACCCCTGGGCCCAGGACAAAGACCTTCTTGACTATGAGGTAGACAGTGACGATGAGTGGG AAGAGGAGGAACCTGGGGAGTCTTTGTCCCACAGTGAGGGGGATGAGGATGACGATGTGGGGGAAGATGA GGATGAGGACGATGGCTTCTTTGTGCCCCATGGGTACTTATCTGAAGATGAAGGCGTGACTGAGGAGTGT GCAGACCCTGAGAACCACAAAGTACACCAGAAGCTGAAGGCCAAGGAGTGGGATGAGCTCCTAGCCAAGG GCAAGCGCTTTCGTGTGCTGCAGCCTGTGCACGTGGGATGTGTATGGGCATCAGAGGCAGCCAACTGCAC GAGTTCCGACTTGAAGCTGCTGCAGCAGTTCACTGCATGCCTGCTGGATGTGGCATCCCCTGATGAGCCA GAACCCGGGGCCTCCAGGAGGGAAAAGAGGGACCAGCATATCCTGGCCCAGTTGCTTCCGCTGCTACATG GCAATGTGAATGGGAGCAAGGTGATCATCCATGAATTCCAGGAGCAATGCCGCAGGGGCCTGCTGACCCT TCCAAGTCCCACCCCACACCTGCAGATGCCCAACTTGGAGGATGCTGTTGCTGTGCCATCCAAGGCCAGG CTGAAGCGCCTTATCTCTGAGAACTCTGCCTATGAGAAGCGGCCCAACTTCCGTATGTGCTGGTATGTGC ACCCAGAGGTGCTCAAGAGCTTTGGCCAGGAGTGCCTGCCAGTGCCCTGCCAGTGGACCTACATCACCAC CATGCCCTCAGCACCCAGGGAAGACAGTGGCAGTGCTTCTACTGAGGGGCCTGGCCAGAGCACACCTATG CTACTCAAGCGGAAGCCAGCGGCCACCATGTGCATCACGCAGTTTATGAAGAAGCGGCGCTATGATGGAC AGGTTGGGTCTGGGGACATGGATGGCTTCCAGGCAGACACAGAAGAGGATGAAGAGGATGACACAGACTG TATGATCATAGATGTGCCAGATGTTGGGAGTGACGTGTCAGAAGCTCCCATACCTGCTCCCACACTTTGC AAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013733 |
| Insert Size | 2736 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013733.3, NP_038761.1 |
| RefSeq Size | 3308 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 27221 |
| UniProt ID | Q9QWF0 |
| Gene Summary | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211155 | Chaf1a (tGFP-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 7,890.00 |
|
| MR211155 | Chaf1a (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 6,672.00 |
|
| MR211155L3 | Lenti ORF clone of Chaf1a (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 9,030.00 |
|
| MR211155L4 | Lenti ORF clone of Chaf1a (mGFP-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 9,030.00 |
