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Ptchd1 (NM_001093750) Mouse Untagged Clone
CAT＃: MC222439
Ptchd1 (untagged) - Mouse patched domain containing 1 (Ptchd1), (10ug)
CNY 11,500.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9630036J22Rik; AI848149; Gm387 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222439 representing NM_001093750
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGGCAGGTTCTGCACAGGGGCTTGAGGACGTGTTTCTCCCGGCTTGGCCACTTCATTGCCAGTC ACCCGGTCTTCTTTGCTTCGGCGCCGGTGCTCATCTCCATCCTGCTCGGCGCCAGCTTCAGCCGCTACCA GGTCGAAGAGAGCGTGGAGCACCTGCTGGCGCCCCAGCACAGCCTAGCCAAGATCGAGCGCAACCTAGTC AACAGCCTCTTCCCGGTCAACCGCTCCAAGCACCGGCTCTACTCGGACCTGCAGACCCCTGGGCGCTACG GCCGGGTCATTGTCACCTCCTACCAGAAAGCCAACATGCTAGACCAACATCACACGGACCTGATCTTAAA GTTGCATACTGCAGTGACCAAGATCCAGGTTCCAAGGCCTGGCTTTAACTATACATTTGCTCACATATGT GTCCTGAATAATGATAAGACTTGCATTGTGGATGACATAGTACACGTCCTGGAAGAGCTAAAGAATGCTC GGGCCACCAATCGGACCAATTTTGCTATCACATATCCGATCACTCACTTAAAAGATGGAAGGGCTGTGTA TAATGGGCACCAACTCGGAGGTGTCACTGTGCACAGCAAGGATCGGGTGAAATCTGCAGAGGCCATTCAG CTTACCTACTATCTGCAATCAATCAACAGTCTCAATGACATGGTAGCTGAGAGGTGGGAGTCCAGCTTCT GTGACACTGTCAAACTATTCCAGAAATCCAACAGCAAAGTCAAAATATACCCGTACACATCCTCATCACT GAGGGAAGATTTCCAGAAGACCAGCCGTGTATCAGAACGTTACCTGGTCACCAGCCTGATCCTGGTAGTC ACCATGGCTATTCTGTGCTGCTCTATGCAGGACTGTGTCCGCAGCAAACCCTGGTTAGGCCTACTTGGGT TGGTGACCATAAGCCTAGCCACTCTCACTGCAGCCGGAATCATCAATCTCACTGGTGGAAAATACAACTC CACCTTCCTGGGAGTCCCTTTCGTCATGCTAGGTCATGGATTATACGGGACTTTTGAAATGTTATCCTCG TGGAGGAAAACTAGAGAGGACCAACATGTTAAAGAGCGAACTGCAGAAGTCTATGCAGACTCCATGCTCT CCTTCTCTCTCACCACTGCCATGTACTTGGTCACCTTCGGCATCGGGGCCAGCCCTTTCACAAACATTGA GGCAGCCAGGATTTTCTGCTGCAACTCCTGTATTGCAATCCTCTTCAACTACCTCTATGTACTCTCATTT TATGGTTCCAGCCTTGTGTTCACTGGCTACATAGAAAACAATTACCAGCATAGTATATTCTGTAGAAAAG TTCCGAAGCCTGATGTATTACAGGAGAAGCCAGCATGGTATAGGTTTCTTCTGACGGCTAGATTCAGTGA GGAAACAGCTGAAGGTGAGGAAGCAAACACTTATGAGAGTCACTTATTGGTATGTTTCCTCAAACGCTAT TACTGTGACTGGATAACCAACACCTATGTCAAGCCTTTTGTAGTCCTCTTTTACCTTATTTATATTTCCT TTGCCTTAATGGGCTATCTGCAGGTCAGTGAAGGGTCAGACCTTAGTAACATCGTAGCAACTGCAACACA AACCATTGAGTACACTACTGCCCACCAAAAGTACTTTAACAACTACAGTCCTGTTATTGGGTTTTACATA TATGAGTCCATAGAATATTGGAACTCTAGTGTCCAAGAAGATGTTCTAGAGTACACTAAGGGTTTTGTGC GGATATCCTGGTTTGAAAGCTATTTAAATTATCTTCGGAAACTCAATGTGTCCACTGACTTGCCTAAGAA AAATTTCACAGACATGTTGAGGAATTCCTTCCTGAAAACTCCTCAATTTTCACATTTTCAAGAGGACATC ATCTTCTCTAAAAAGTACAATGATGAGGTTGATGTAGTGGCCTCTAGAATGTTTTTGGTGGCCAAGACTA TGGAAACAAACAGAGAAGAACTTTATGATCTCTTGGAGACTCTGAGGAGGCTTTCAGTCACCTCCAAGGT GAAGTTCATTGTCTTTAATCCGTCTTTTGTGTACATGGATCGATATGCCTCCTCTCTGGGAGCCCCCCTG CACAATTCCTGCATCAGTGCTTTGTTCCTGCTCTTCTTCTCGGCATTCCTGGTAGCAGATTCGCTGATCA ACGTCTGGATCACTCTCACGGTTGTGTCTGTGGAGTTTGGAGTGATAGGCTTCATGACATTATGGAAAGT CGAACTGGATTGCATTTCTGTGCTATGCTTAATTTATGGAATTAACTACACAATTGACAACTGTGCTCCA CTGTTATCCACATTTGTTCTAGGCAAGGATTTTACAAGAACTAAATGGGTTAAAAATGCCCTGGAAGTGC ATGGGGTAGCTATTTTACAGAGTTACCTCTGTTATATTGTTGGTCTATTTCCTCTTGCAGCTGTGCCTTC AAATCTGACCTGTACACTGTTCAGGTGCTTGTTTTTAATAGCATTTGTCACCTTCTTTCACTGCTTTGCC ATTTTACCTGTGATACTTACTTTCCTGCCACCCTCTAAGAAAAAAAGGAAAGAGAAGAAAAATCCTGAAA ACCGGGAGGAAATTGAGTGTGTAGAAATGGTAGATATTGATAGTACTCGAGTGGTTGACCAAATAACCAC AGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001093750 |
| Insert Size | 2667 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001093750.1, NP_001087219.1 |
| RefSeq Size | 6489 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 211612 |
| UniProt ID | Q14B62 |
| Gene Summary | Required for the development and function of the thalamic reticular nucleus (TRN), a part of the thalamus that is critical for thalamocortical transmission, generation of sleep rhythms, sensorimotor processing and attention.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219209 | Ptchd1 (tGFP-tagged) - Mouse patched domain containing 1 (Ptchd1), (10ug) |
CNY 9,690.00 |
|
| MR219209 | Ptchd1 (Myc-DDK-tagged) - Mouse patched domain containing 1 (Ptchd1) |
CNY 9,752.00 |
|
| MR219209L3 | Lenti ORF clone of Ptchd1 (Myc-DDK-tagged) - Mouse patched domain containing 1 (Ptchd1) |
CNY 10,740.00 |
|
| MR219209L4 | Lenti ORF clone of Ptchd1 (mGFP-tagged) - Mouse patched domain containing 1 (Ptchd1) |
CNY 10,740.00 |
