Ndst2 (NM_010811) Mouse Untagged Clone

CAT#: MC222370

Ndst2 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (Ndst2), (10ug)



  "NM_010811" in other vectors (4)

CNY 11,310.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Mndns; NDST-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222370 representing NM_010811
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCAGCTGTGGAAGGTGGTGCGCCCTGCTCGGCAACTGGAACTGCACCGCCTCATACTGCTGCTGA
TTGGTTTCAGTCTTGTCTCCATGGGATTCCTGGCTTACTACGTGTCCACCAGCCCCAAGGCCAAGGAACC
CTTGCCTCTGCCCTTGGGAGACTGTAGTAGCAGTGGGGCAGCTGGCCCTGGCCCTGCACGGCCACCAGTC
CCACCTCGACCCCAAAGGCCTCCAGAAACAACTCGAACGGAACCTGTGGTCCTTGTGTTTGTGGAAAGTG
CATACTCACAACTGGGGCAGGAGATTGTGGCCATCTTGGAATCTAGTCGCTTTCGTTATAGCACTGAGCT
AGCACCTGGCCGAGGGGATATGCCCACACTGACTGATCATACCCATGGCCGCTATGTCTTAGTCATCTAT
GAGAACCTTCTAAAGTATGTGAACTTAGATGCCTGGAGCCGGGAACTGCTGGACCGGTACTGTGTGGAGT
ATGGCGTGGGTATCATTGGCTTTTTCCGAGCCCGCGAGCACAGCCTGCTGAGTGCCCAGCTCAAGGGCTT
TCCCCTTTTCCTACACTCAAACCTGGGGCTCCGGGACTACCAAGTGAATCCTTCCGCCCCTCTACTACAT
CTCACCCGCCCTAGCCGCCTGGAGCCAGGACCACTGCCTGGTGATGACTGGACCATCTTCCAGTCCAATC
ACAGCACTTATGAGCCCGTGCTTATTGCCAGCCATCGGCCAGCCGAGCTCTCCATGCCAGGACCAGTTCT
TCGGCGGGCCCGGCTCCCCACCGTGGTGCAGGACTTGGGGCTTCATGATGGCATACAGCGGGTGCTGTTT
GGACATGGCCTTTCTTTTTGGCTTCACAAACTTGTTTTTGTTGATGCTGTTGCATACCTCACCGGGAAGC
GCCTCTGCCTGGACCTTGACCGCTACATCTTAGTAGATATCGATGACATCTTTGTGGGCAAAGAAGGTAC
TCGCATGAAGGTGGCTGATGTTGAGGCTTTGTTGACCACTCAGAACAAACTCAGGACCTTAGTCCCCAAC
TTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACCGGGACAGAAGAGGAGGATGCTGGGGACG
ACATGCTGCTGAAGCACCGCAGGGAGTTCTGGTGGTTCCCTCACATGTGGAGCCACATGCAGCCACACCT
CTTCCACAACCGCTCTGTGCTAGCTGACCAGATGAGGCTCAACAAACAGTTTGCTTTGGAGCATGGGATT
CCTACGGATCTGGGGTATGCTGTGGCCCCCCACCACTCTGGCGTGTACCCCATCCACTCGCAGCTCTATG
AGGCCTGGAAATCTGTCTGGGGCATCCAGGTGACCAGCACTGAGGAGTACCCCCATCTGCGTCCTGCTCG
CTACCGCCGTGGCTTCATTCACAATGGCATCATGGTGCTCCCCCGGCAAACATGTGGCCTCTTCACCCAC
ACGATCTTCTATAATGAGTATCCTGGAGGTTCTCGTGAACTAGATCGAAGCATCCGAGGTGGAGAGCTCT
TTCTGACAGTGCTGCTTAATCCGATCAGTGTCTTTATGACCCATCTGTCAAACTATGGAAATGACCGGCT
GGGCCTGTACACCTTTGAGAGCCTGGTGCGCTTCCTCCAGTGCTGGACACGACTGCGCCTACAGACCCTT
CCTCCTGTCCCTCTTGCACAAAAGTACTTTGAACTTTTTCCTCAGGAACGAAGCCCCCTGTGGCAGAATC
CCTGTGATGACAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGACTCCCCAAGTTCCT
CATTGTGGGACCACAGAAGACAGGCACCACGGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTCACA
AGCAGTTTTCCCAGCCCCAGCACCTTTGAGGAGATTCAGTTCTTCAATGGCCCTAATTACCACAAGGGGA
TCGACTGGTACATGGACTTCTTCCCTGTTCCTTCCAATGCCAGCACTGACTTCCTGTTTGAAAAAAGTGC
CACCTACTTTGACTCGGAGGTCGTACCACGGCGGGGGGCTGCCCTTCTGCCAAGAGCCAAGATAATCACT
GTGCTTATCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCACCAGCGAGCACATGGAGACCCCATTG
CTCTGAACTACACCTTCTACCAAGTGATTTCAGCTTCTTCTCAGGCCCCTCTGCTACTTCGCTCCTTGCA
GAACCGTTGTCTTGTTCCTGGCTACTATTCTACCCATCTTCAACGCTGGCTGACTTACTATCCCTCTGGA
CAGTTGCTGATTATGGATGGGCAGGAGCTTCGTGTCAACCCGGCAGCCTCAATGGAGATCATCCAGAAGT
TCCTGGGTATCACCCCCTTTCTGAACTACACGAGGACCCTCAGGTTTGATGAAGATAAAGGATTTTGGTG
CCAGGGCCTTGAAGGTGGTAAGACTCGCTGTCTTGGGAGAAGCAAAGGCCGGAGGTACCCAGACATGGAC
ATGGAGTCTCGCCTTTTCCTTACGGATTTTTTCCGGAACCATAATTTGGAGCTATCAAAGCTGCTGAGTC
GGCTTGGCCAGCCAGCCCCCTTGTGGCTTCGGGAAGAATTGCAACATTCCAGTGTGGGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010811
Insert Size 2652 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010811.2, NP_034941.2
RefSeq Size 3907 bp
RefSeq ORF 2652 bp
Locus ID 17423
UniProt ID P52850
Gene Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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