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Ube3a (NM_011668) Mouse Untagged Clone
CAT＃: MC222317
Ube3a (untagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 2, (10ug)
CNY 11,210.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a; mKIAA4216 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222317 representing NM_011668
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGCTTGTAAAAGATCACCAGGAGAATCCCAGTCTGAGGACATTGAAGCTAGCCGAATGAAGC GAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATGAGGCCTG CACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTATTAAAGCC CTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTT ACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGGAAAAGATTT TAAAGATGTGATTTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGAGAGAGTGAGGAT TATTCCCCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCTGAGCTTTCGGA AAGTCAAACAGCACACAAAGGAGGAATTGAAATCTCTTCAAGAAAAGGATGAAGACAAGGATGAAGATGA AAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAGGATGGGT GATAGTTCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATTGATGCTA TTAGAAGGGTCTACAGCAGTTTGCTCGCTAATGAAAAATTAGAAACTGCCTTCCTGAATGCACTTGTATA TCTGTCACCTAACGTGGAATGTGATTTGACATATCATAATGTGTATACTCGAGATCCTAATTATCTCAAT TTGTTCATTATTGTAATGGAGAATAGTAATCTCCACAGTCCTGAATATCTGGAAATGGCGTTGCCATTAT TTTGCAAAGCTATGTGTAAGCTACCCCTTGAAGCTCAAGGAAAACTGATTAGGCTGTGGTCTAAATACAG TGCTGACCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAGCAATGAA TTTAATAGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAAATGGTTT ACTATGCAAATGTAGTGGGAGGGGATGTGGACACAAATCATAATGAGGAAGATGATGAAGAACCCATACC TGAGTCCAGCGAATTAACACTTCAGGAGCTTCTGGGAGATGAAAGAAGAAATAAGAAAGGTCCTCGAGTG GATCCACTAGAAACCGAACTTGGCGTTAAAACTCTAGACTGTCGAAAACCACTTATCTCCTTTGAAGAAT TCATTAATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACCTTTTTCAAAGTTGAAACAGA GAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATTATATTAT GACAATAGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGACAGCAGT TGAATCCGTATTTGAGACTCAAAGTCAGACGTGACCATATTATAGATGATGCACTGGTCCGGCTAGAGAT GATTGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTA GATGAGGGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATTTTTAATCCAGATATTGGTA TGTTCACATATGATGAAGCTACGAAATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTT TACTCTGATTGGCATAGTCCTGGGTCTGGCTATTTACAATAATTGTATACTGGATGTCCATTTTCCCATG GTTGTATACAGGAAGCTAATGGGGAAAAAAGGAACCTTTCGTGACTTGGGAGACTCTCACCCAGTTTTAT ATCAGAGTTTAAAGGATTTATTGGAATATGAAGGGAGTGTGGAAGATGATATGATGATCACTTTCCAGAT ATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAATTCCAATT ACAAATGAAAACAGGAAGGAATTTGTCAATCTCTATTCAGACTACATTCTCAATAAATCTGTAGAAAAAC AATTCAAGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCGCCCTTAAAATACTTATTCAGACC AGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAGGCACTAGAAGAAACTACAGAG TATGACGGTGGCTATACGAGGGAATCTGTTGTGATTAGGGAGTTCTGGGAAATTGTTCATTCGTTTACAG ATGAACAGAAAAGACTCTTTCTGCAGTTTACAACAGGCACAGACAGAGCACCTGTTGGAGGACTAGGAAA ATTGAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAAT GTCCTTTTACTTCCGGAATATTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACATATG CCAAAGGATTTGGCATGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011668 |
| Insert Size | 2613 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011668.2, NP_035798.2 |
| RefSeq Size | 5097 bp |
| RefSeq ORF | 2613 bp |
| Locus ID | 22215 |
| UniProt ID | O08759 |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:20211139, PubMed:24728990). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo (By similarity). Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (PubMed:20211139). Synergizes with WBP2 in enhancing PGR activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A coding-independent function of an alternative Ube3a transcript during neuronal development
,Valluy, J;Bicker, S;Aksoy-Aksel, A;Lackinger, M;Sumer, S;Fiore, R;Wüst, T;Seffer, D;Metge, F;Dieterich, C;Wöhr, M;Schwarting, R;Schratt, G;,
Nat. Neurosci.
,PubMed ID 25867122
[Ube3a]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224630 | Ube3a (tGFP-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a) transcript variant 2, (10ug) |
CNY 7,976.00 |
|
| MR224630 | Ube3a (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 2 |
CNY 6,376.00 |
|
| MR224630L3 | Lenti ORF clone of Ube3a (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 2 |
CNY 9,030.00 |
|
| MR224630L4 | Lenti ORF clone of Ube3a (mGFP-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 2 |
CNY 8,776.00 |
