Axin1 (NM_009733) Mouse Untagged Clone

CAT#: MC222249

Axin1 (untagged) - Mouse axin 1 (Axin1), transcript variant 2, (10ug)



  "NM_009733" in other vectors (4)

CNY 11,120.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Axin1 Antibody - N-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI316800; Axin; Fu; fused; Kb; Ki; kinky; knobbly
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222249 representing NM_009733
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGTCCCAAAATGAATGTCCAGGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAG
ATGCCCCCCGGCCCCCAGTGCCTGGAGAAGAGGGAGAACTGGTATCTACTGATTCGAGGCCTGTCAACCA
CAGTTTCTGTTCTGGGAAAGGTACCAGCATTAAAAGTGAGACCTCAACAGCCACCCCAAGACGTTCAGAT
CTGGATCTGGGATATGAGCCCGAGGGCAGTGCCTCCCCCACCCCACCATATTTGAGGTGGGCTGAGTCAC
TGCATTCCTTACTGGATGACCAAGATGGGATCAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGC
TGACCTGCTGGACTTCTGGTTTGCCTGCAGTGGCTTCAGGAAGCTTGAGCCCTGTGACTCAAATGAGGAA
AAGAGGCTGAAGCTGGCAAGAGCCATCTACCGAAAGTACATCCTGGATAGCAATGGCATTGTGTCCAGAC
AAACCAAGCCAGCCACTAAGAGCTTCATAAAGGACTGTGTCATGAAGCAGCAGATAGATCCTGCCATGTT
TGACCAGGCACAGACAGAAATCCAGTCCACCATGGAGGAGAATACCTACCCTTCCTTTCTTAAGTCTGAC
ATTTATTTGGAGTACACAAGGACAGGCTCAGAGAGTCCGAAGGTCTGCAGTGACCAGAGCTCAGGGTCTG
GAACAGGGAAGGGCATGTCTGGATACCTGCCCACTTTGAATGAGGATGAAGAATGGAAATGTGACCAAGA
TGCAGATGAGGATGATGGCCGAGACCCTCTCCCCCCCAGCAGGCTCACCCAGAAGCTGCTATTGGAGACT
GCTGCCCCGAGGGCCCCCTCAAGTAGACGGTACAACGAAGGCAGAGAGCTCAGGTATGGATCTTGGAGGG
AGCCCGTCAACCCCTACTACGTCAACTCTGGCTATGCCCTGGCCCCAGCCACCAGTGCCAATGACAGTGA
GCAGCAGAGCCTGTCCAGTGATGCTGACACGCTATCCCTTACGGACAGTAGTGTGGATGGAATCCCCCCA
TACAGGATCCGTAAGCAGCACCGAAGGGAGATGCAGGAGAGTATCCAAGTCAATGGGCGGGTACCTCTAC
CTCACATTCCTCGCACTTACCGAATGCCAAAGGAGATCCGGGTAGAGCCACAGAAATTTGCTGAAGAGCT
TATTCACCGTCTAGAGGCTGTCCAGCGCACTCGAGAGGCTGAAGAAAAGTTGGAGGAACGGCTGAAGCGT
GTACGCATGGAGGAAGAAGGGGAGGATGGTGAAATGCCTTCTGGCCCCATGGCAAGTCACAAGCTGCCTT
CTGTCCCAGCTTGGCACCATTTCCCACCCCGCTATGTGGATATGGGCTGCTCTGGACTGCGGGATGCCCA
TGAGGAGAATCCTGAGAGCATCCTGGATGAGCACGTGCAAAGGGTCATGAGGACACCTGGCTGCCAGTCA
CCTGGCCCAGGCCACCGCTCTCCTGACAGTGGGCATGTGGCTAAGACTGCAGTGCTAGGGGGTACAGCCT
CCGGGCATGGGAAGCATGTTCCTAAGTTAGGGTTGAAGCTGGATACAGCTGGCCTGCACCATCATAGACA
TGTCCACCACCATGTTCACCATAATTCAGCTAGACCTAAGGAGCAAATGGAGGCTGAAGTTGCCCGCAGG
GTCCAGAGCAGCTTCTCGTGGGGCCCAGAAACACATGGTCATGCCAAGCCCCGGAGCTATTCCGAGAACG
CAGGCACCACCCTCAGTGCTGGGGATTTGGCCTTTGGTGGTAAAACTAGTGCACCTTCCAAAAGAAACAC
CAAGAAGGCTGAATCTGGGAAGAATGCCAATGCTGAGGTACCCAGTACCACAGAGGACGCTGAGAAGAAC
CAGAAGATCATGCAGTGGATCATTGAGGGAGAGAAGGAGATCAGTAGACACCGGAAGGCAGGCCATGGGT
CTTCTGGGTTGAGGAAGCAGCAGGCCCATGAAAGCTCCAGGCCCTTGTCCATCGAGCGTCCTGGGGCCGT
GCACCCCTGGGTCAGCGCTCAGCTTCGGAATTCTGTCCAGCCTTCTCATCTTTTCATCCAAGATCCCACA
ATGCCACCCAATCCAGCCCCTAATCCCCTGACCCAGCTGGAAGAGGCCCGCAGGCGTTTGGAAGAAGAAG
AAAAGAGAGCAAACAAACTGCCCTCCAAGCAGAGGTATGTGCAGGCAGTCATGCAGCGGGGACGCACCTG
TGTCAGGCCAGCTTGTGCACCGGTGCTGAGTGTGGTACCAGCCGTGTCGGACTTGGAACTCTCCGAGACA
GAGACAAAATCACAAAGAAAGGCAGGTGGCGGGAGTGCACCACCATGTGACAGCATTGTTGTGGCCTACT
ATTTCTGTGGGGAACCCATCCCCTACCGGACCCTGGTGAGGGGCCGTGCTGTCACCCTGGGCCAGTTCAA
GGAGCTGCTAACCAAGAAGGGGAGCTACAGATACTACTTTAAGAAAGTGAGTGATGAGTTTGACTGTGGT
GTGGTATTTGAGGAAGTACGGGAGGATGAGGCCGTCTTGCCTGTCTTTGAAGAAAAGATCATCGGCAAGG
TGGAAAAGGTGGACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009733
Insert Size 2607 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009733.2, NP_033863.2
RefSeq Size 3809 bp
RefSeq ORF 2607 bp
Locus ID 12005
UniProt ID O35625
Gene Summary Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant contains an alternate in-frame exon in the 3' coding region compared to variant 1, resulting in a longer protein (isoform 2) compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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