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Hif1a (NM_010431) Mouse Untagged Clone
CAT＃: MC221924
Hif1a (untagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a), (10ug)
CNY 9,192.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA959795; bHLHe7; bHLHe78; HIF-1; HIF-1-alpha; HIF1; HIF1-alpha; HIF1alpha; MO; MOP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221924 representing NM_010431
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAAGATGAGTTCTGAACGTCGAAAAGAAAAGTCTAGAG ATGCAGCAAGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCC CCACAATGTGAGCTCACATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTATTTACGTGTGAGA AAACTTCTGGATGCCGGTGGTCTAGACAGTGAAGATGAGATGAAGGCACAGATGGACTGTTTTTATCTGA AAGCCCTAGATGGCTTTGTGATGGTGCTAACAGATGACGGCGACATGGTTTACATTTCTGATAACGTGAA CAAATACATGGGGTTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAAGGGAAAGAACTAAACACAC AGCGGAGCTTTTTTCTCAGAATGAAGTGCACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTCAGC AACGTGGAAGGTGCTTCACTGCACGGGCCATATTCATGTCTATGATACCAACAGTAACCAACCTCAGTGT GGGTACAAGAAACCACCCATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCGTCAAATATTG AAATTCCTTTAGATAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGATGA AAGAATTACTGAGTTGATGGGTTATGAGCCGGAAGAACTTTTGGGCCGCTCAATTTATGAATATTATCAT GCTTTGGATTCTGATCATCTGACCAAAACTCACCATGATATGTTTACTAAAGGACAAGTCACCACAGGAC AGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAATAC GAAGAACTCCCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGACTTG ATTTTCTCCCTTCAACAAACAGAATCTGTGCTCAAACCAGTTGAATCTTCAGATAAGAAGATGACTCAGC TGTTCACCAAAGTTGAATCAGAGGCTACAAGCTGCCTTTTTGATAAGCTTAAGAAGGAGCCTGATGCTCT CACTCTGCTGGCTCCAGCTGCCGGCGACACCATCATCTCTCTGGATTTTGGCAGCGATGACACAGAAACT GAAGATCAACAACTTGAAGATGTTCCATTATATAATGATGTAATGTTTCCCTCTTCTAATGAAAAATTAA ATATAAACCTGGCAATGTCTCCTTTACCTTCATCGGAAACTCCAAAGCCACTTCGAAGTAGTGCTGATCC TGCACTGAATCAAGAGGTTGCATTAAAATTAGAATCAAGTCCAGAGTCACTGGGACTTTCTTTTACCATG CCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGTTCACCTGAGAGACTTC TTCAGGAAAACGTAAACACTCCTAACTTTTCCCAGCCTAACAGTCCCAGTGAATATTGCTTTGATGTGGA TAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGAAAAACTGTTTGCTGAAGACACAGAGGCAAAG AATCCATTTTCAACTCAGGACACTGATTTAGACTTGGAGATGCTGGCTCCCTATATCCCAATGGATGATG ATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAGAGAGCAATTCTCCAAGCCCTCCAAGTATGAG CACAGTTACTGGGTTCCAGCAGACCCAGTTACAGAAACCTACCATCACTGCCACTGCCACCACAACTGCC ACCACTGATGAATCAAAAACAGAGACGAAGGACAATAAAGAAGATATTAAAATACTGATTGCATCTCCAT CTTCTACCCAAGTACCTCAAGAAACGACCACTGCTAAGGCATCAGCATACAGTGGCACTCACAGTCGGAC AGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAACAGACAGACAAAGCTCATCCAAGGAGCCTTAAC CTGTCTGCCACTTTGAATCAAAGAAATACTGTTCCTGAGGAAGAATTAAACCCAAAGACAATAGCTTCGC AGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCTCCCTTTTTCAAGCAGCAGGAATTGGAACATT ATTGCAGCAACCGGGTGACTGTGCACCTACTATGTCACTTTCCTGGAAACGAGTGAAAGGATTCATATCT AGTGAACAGAATGGAACGGAGCAAAAGACTATTATTTTAATACCCTCCGATTTAGCATGCAGACTGCTGG GGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCAGTTACGATTGTGAAGTTAATGCTCCCATACA AGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010431 |
| Insert Size | 2511 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026139, AAH26139 |
| RefSeq Size | 4775 bp |
| RefSeq ORF | 2511 bp |
| Locus ID | 15251 |
| UniProt ID | Q61221 |
| Gene Summary | This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210895 | Hif1a (tGFP-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 10,784.00 |
|
| MR210895 | Hif1a (Myc-DDK-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 9,184.00 |
|
| MR210895L1 | Lenti ORF clone of Hif1a (Myc-DDK-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 11,584.00 |
|
| MR210895L2 | Lenti ORF clone of Hif1a (mGFP-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 10,260.00 |
|
| MR210895L3 | Lenti ORF clone of Hif1a (Myc-DDK-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 10,260.00 |
|
| MR210895L4 | Lenti ORF clone of Hif1a (mGFP-tagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a) |
CNY 11,584.00 |
