Mcm6 (NM_008567) Mouse Untagged Clone

CAT#: MC221835

Mcm6 (untagged) - Mouse minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), (10ug)



  "NM_008567" in other vectors (4)

CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms ASP-l1; D1Wsu22e; Mcmd6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221835 representing NM_008567
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTCGCAGCGGCCGCGGAGCCCGGCGCCGGAAGCCAGCACCCGGAGGTGCGCGACGAGGTGGCCG
AGAAATGCCAGAAGCTGTTCCTAGACTTCCTGGAAGAGTTCCAGGGTAGTGATGGAGAAATTAAATACTT
GCAGTTTGCAGAGGAGCTAATTCGTCCTGAGAGAAACACGCTGGTTGTGAGTTTTGCAGACCTGGAACAA
TTTAACCAACAACTTTCTACCACCATTCAGGAAGAGTTCTATAGAGTCTACCCTTACCTGTGTCGAGCCT
TGAAGACCTTTGTCAAAGACCGAAAGGAGATCCCTTTTGCTAAGGATTTTTATGTTGCATTCCAAGACCT
ACCTACTAGACACAAGATTCGTGAGCTCACTTCATCCAGGATTGGGCTGCTCACCCGAATCAGTGGCCAG
GTGGTGCGCACTCACCCAGTTCACCCTGAGCTTGTGAGTGGAACTTTCCTGTGCCTTGACTGTCAAACAG
TGATTAAGGACGTAGAGCAGCAGTTCAAATACACACAGCCGAATATCTGCCGAAATCCAGTGTGTGCCAA
CAGGAAGAGGTTCCTTCTAGACACTAATAAATCAAGATTTGTTGATTTTCAAAAGGTTCGCATTCAAGAG
ACTCAAGCGGAACTTCCCCGGGGAAGTATTCCCCGGAGTTTAGAGGTTATCCTGAGAGCTGAAGCTGTGG
AGTCAGCCCAAGCTGGTGACAGATGTGATTTCACAGGGGCACTGATTGTTGTGCCTGATGTCTCCAAGCT
TAGCACACCAGGAGCACGTGCAGAGACTAACTCCCGAGTCAGTGGAGCTGATGGGTATGAGACAGAAGGC
ATTCGAGGGCTCCGGGCCCTTGGTGTCAGAGATCTGTCATACAGGCTGGTCTTCCTTGCCTGCCATGTTG
CACCAACCAACCCAAGGTTTGGAGGGAAAGAACTCAGAGATGAGGAACAGACCGCTGAGAGCATTAAGAA
CCAAATGACGGTGAAGGAATGGGAAAAGGTGTTTGAAATGAGTCAGGACAAAAACCTGTACCACAATCTC
TGCACGAGCCTCTTCCCTACTATACATGGCAATGATGAAGTAAAACGCGGTGTCTTACTGATGTTGTTCG
GTGGTGTTCCTAAGACAACAGGGGAAGGAACCTCTCTTCGTGGGGATATAAATGTTTGCATTGTTGGTGA
TCCAAGTACAGCTAAGAGCCAATTTCTCAAGCACGTGGACGAGTTCAGTCCCAGAGCTGTCTATACCAGT
GGCAAAGCCTCCAGCGCTGCCGGCTTAACAGCAGCTGTGGTGAGAGATGAAGAATCTCACGAATTTGTCA
TTGAGGCTGGAGCGTTGATGCTGGCTGATAATGGTGTCTGTTGTATTGATGAATTTGATAAGATGGACAT
GCGGGACCAAGTTGCTATTCACGAAGCTATGGAGCAGCAGACCATCTCTATCACGAAAGCAGGAGTGAAG
GCAACTCTGAATGCCAGGACATCCATTTTAGCTGCAGCAAACCCTGTCAGCGGACACTATGACAGATCAA
AATCTTTGAAACAGAACATCAATCTGTCAGCGCCCATCATGTCCCGCTTTGATCTCTTCTTCATTCTGGT
GGATGAATGCAATGAGGTGACAGATTATGCTATTGCCAGGCGCATAGTAGACTTGCATTCAAGAATTGAA
GAATCGATCGATCGAGTTTATTCCCTTGATGATATAAGAAGGTACCTTCTCTTTGCCCGACAGTTTAAAC
CCAAGATTTCCAAAGAGTCAGAAGACTTCATTGTGGAGCAGTACAAACGTCTCCGCCAGAGGGATGGCTC
TGGGGTCACCAAGTCCTCGTGGAGAATCACCGTGCGACAGCTTGAGAGCATGATCCGACTCTCAGAATCA
ATGGCCCGCATGCACTGCTGTGACGAGGTTCAGCCTAAACATGTGAAGGAAGCTTTTCGATTACTGAATA
AATCAATCATCCGTGTAGAGACGCCTGATGTCAATCTAGATCAAGAGGAAGAGATCCAGATGGAGACGGA
TGAGGGACAAGGAGGCGTCAATGGTCATGCCGACAGCCCTGCCCCTGTGAATAGGTTCAACGGCTCCAGT
GAAGATGCCAGCCAGGAGACTGTTTCCAAGCCCTCCCTGAGGCTGGGCTTTGCTGAATACTGCCGAATCT
CGAACCTCATCGTGCTCCACCTCAGGAAAATGGAAGAAGAGGAGGACGAGTCGGCACTAAAGAGAAGCGA
GCTTGTCAACTGGTACCTGAAGGAAATCGAATCAGAGATAGACTCTGAAGAAGAGCTCATAAATAAAAAG
ACGATCATTGAGAAGGTTGTTCATCGTCTCACACACTACGATCACGTTCTGATTGAGCTCACCCAGGCTG
GGCTGAAAGGCTCCAGTGAAGGAAGCGAGAGCTATGAGGAAGACCCCTACCTGGTAGTCAACCCTAACTA
CTTGCTTGAAGATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_008567
Insert Size 2466 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008567.2, NP_032593.1
RefSeq Size 2966 bp
RefSeq ORF 2466 bp
Locus ID 17219
UniProt ID P97311
Gene Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...