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Braf (NM_139294) Mouse Untagged Clone
CAT＃: MC221774
Braf (untagged) - Mouse Braf transforming gene (Braf), (10ug)
CNY 5,896.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; Braf-2; Braf2; C87398; C230098H17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221774 representing NM_139294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGAGTGGCGGCGGTGGCAGCAGCAGCGGTGGCGGCGGCGGCGGTGGCGGCGGCGGTGGCG GTGGCGACGGCGGCGGCGGCGCCGAGCAGGGCCAGGCTCTGTTCAATGGCGACATGGAGCCGGAGGCCGG CGCTGGCGCCGCGGCCTCTTCGGCTGCGGACCCGGCCATTCCTGAAGAGGTATGGAATATCAAGCAAATG ATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGACAAATTTGGTGGAGAGCATAACCCACCATCAA TATACCTGGAGGCCTATGAAGAGTACACCAGCAAGCTAGATGCCCTTCAGCAAAGAGAACAGCAGCTTTT GGAATCCCTGGTTTTTCAAACTCCCACAGATGCATCACGGAACAACCCCAAGTCACCACAGAAACCTATC GTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCCGCAAGATGTGGTGTTACAGTTCGAGACA GTCTAAAGAAAGCACTGATGATGAGAGGTCTCATCCCAGAATGCTGTGCTGTTTACAGAATTCAGGATGG AGAGAAGAAACCAATTGGCTGGGACACGGACATTTCCTGGCTTACTGGAGAGGAGTTACATGTTGAAGTA CTGGAGAATGTCCCACTTACAACACACAACTTTGTACGGAAAACTTTTTTCACCTTAGCATTTTGTGACT TTTGCCGAAAGCTGCTTTTCCAGGGTTTCCGTTGTCAAACATGTGGTTATAAATTTCACCAGCGTTGTAG TACAGAGGTTCCACTGATGTGTGTAAATTATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAG CATCACCCAGTACCACAGGAGGAGGCCTCCTTCCCAGAGACTGCCCTTCCATCTGGATCCTCTTCCGCAC CCCCCTCAGACTCTACTGGGCCCCAAATCCTCACCAGTCCATCTCCTTCAAAATCCATTCCAATTCCACA GCCCTTCCGACCAGCAGATGAAGATCATCGCAATCAGTTTGGGCAACGAGACCGGTCCTCCTCAGCTCCC AATGTTCATATAAACACAATTGAGCCTGTGAATATCGATGAAAAATTCCCAGAAGTGGAATTACAGGATC AAAGGGATTTGATTAGAGACCAGGGGTTTCGTGGTGATGGAGCCCCCTTGAACCAACTGATGCGCTGTCT TCGGAAATACCAATCCCGGACTCCCAGCCCCCTCCTCCATTCTGTCCCCAGTGAAATAGTGTTTGATTTT GAGCCTGGCCCAGTGTTCAGAGGGTCAACCACAGGCTTGTCCGCCACCCCGCCTGCCTCATTACCTGGCT CACTCACTAACGTGAAAGCCTTACAGAAATCTCCAGGTCCTCAGCGGGAAAGGAAGTCATCTTCTTCCTC ATCCTCGGAGGACAGAAGTCGGATGAAAACACTTGGTAGAAGAGATTCAAGTGATGACTGGGAGATTCCT GATGGACAGATTACAGTGGGACAGAGAATTGGATCTGGGTCATTTGGAACTGTCTACAAGGGAAAGTGGC ATGGTGATGTGGCAGTGAAAATGTTGAATGTGACAGCACCCACACCTCAACAGCTACAGGCCTTCAAAAA TGAAGTAGGAGTGCTCAGGAAAACTCGACATGTGAATATCCTCCTTTTCATGGGCTATTCTACAAAGCCA CAACTGGCAATTGTTACACAGTGGTGTGAGGGCTCCAGCTTATATCACCATCTCCACATCATTGAGACCA AATTTGAGATGATCAAACTTATAGATATTGCTCGGCAGACTGCACAGGGCATGGATTACTTACACGCCAA GTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACGGTAAAAATAGGT GACTTTGGTCTAGCCACAGTGAAATCTCGGTGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCTA TTTTGTGGATGGCACCAGAAGTAATCAGAATGCAAGATAAAAACCCGTATAGCTTTCAGTCAGACGTGTA TGCGTTTGGGATTGTTCTGTACGAACTGATGACCGGCCAGCTACCTTATTCAAACATCAACAACAGGGAT CAGATAATTTTTATGGTGGGACGAGGATACCTATCTCCAGATCTCAGTAAGGTACGGAGTAACTGTCCAA AAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAGAAAAGAGACGAGAGACCACTCTTTCCCCAAAT TCTCGCCTCCATTGAGCTGCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCTTCCTTG AATCGGGCTGGTTTCCAAACAGAAGATTTTAGTCTGTATGCTTGTGCTTCTCCGAAAACACCCATCCAAG CAGGGGGATATGGAGAATTTGCAGCCTTCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_139294 |
| Insert Size | 2415 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139294.5, NP_647455.3 |
| RefSeq Size | 9728 bp |
| RefSeq ORF | 2415 bp |
| Locus ID | 109880 |
| UniProt ID | P28028 |
| Gene Summary | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223023 | Braf (tGFP-tagged) - Mouse Braf transforming gene (Braf), (10ug) |
CNY 7,890.00 |
|
| MR223023 | Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf) |
CNY 8,832.00 |
|
| MR223023L1 | Lenti ORF clone of Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf) |
CNY 11,232.00 |
|
| MR223023L2 | Lenti ORF clone of Braf (mGFP-tagged) - Mouse Braf transforming gene (Braf) |
CNY 9,030.00 |
|
| MR223023L3 | Lenti ORF clone of Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf) |
CNY 9,030.00 |
|
| MR223023L4 | Lenti ORF clone of Braf (mGFP-tagged) - Mouse Braf transforming gene (Braf) |
CNY 11,232.00 |
