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Fign (NM_021716) Mouse Untagged Clone
CAT＃: MC221381
Fign (untagged) - Mouse fidgetin (Fign), (10ug)
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | fi; fidget |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221381 representing NM_021716
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGTAGCACCAGTGTTTATGGCTTGAAGATGCAGTGGACGCCGGAGCATGCCCAGTGGCCAGAAC AGCACTTTGACATCACCTCGACCACTCGGTCTCCCGCCCACAAAGTTGAAGCCTATAGAGGTCATTTACA GCGCACCTACCAGTATGCCTGGGCGAATGATGACATATCTGCTCTGACTGCGTCCAACCTTCTGAAAAAA TATGCAGAGAAGTATTCTGGCATTTTGGAAGGTCCCGTAGACCGACCTGTACTCAGCAATTATTCAGACA CACCATCAGGACTCGTGAATGGTCGGAAAAATGATAGCGAACCCTGGCAGCCTTCCTTGAATTCAGAAGC CGTTTATCCTATGAACTGTGTCCCGGATGTCATCACTGCCAGCAAAGCTGGTGTCAGCTCAGCCCTCCCT CCAGTAGACGTCTCGGCAAGTATAGGGAGCTCCCCTGGGGTGGCCAGCAACCTGACAGAGCCAAGTTACT CAAGTAGTACCTGTGGAAGCCACACAGTACCTAGTCTTCATGCGGGGCTCCCATCTCAGGAATATGCTCC GGGATACAACGGCTCATATTTGCATTCTACCTACAGCAGCCAGGCCACACCTGCACTTCCTTCGCCTCAT CCGTCCCCGTTGCATAGTTCTGGGCTTTTGCAGCCACCACCCCCTCCTCCTCCCCCACCAGCCCTGGTCC CAGGCTACAACGGGACTTCCAATCTCTCCAGTTACAGTTATCCCTCTGCTAGCTATCCTCCTCAGACTGC CGTGGGGTCTGGGTACAGCCCTGGCGGAGCACCCCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCT GCTCCCACCCCTCTGCCCCCCACTACCGTTCCTGGCTACACCTACCAAGGTCATGGTTTGACACCCATTG CACCCTCTGCTCTGACCAACAATTCAGCAAGTTCTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGG AGACATGGACTCCAGTTACGGAAATTACAGCTATGGCCAACAGAGATCGACACAGAGTCCTATGTACCGA ATGCCCGACAACAGCATTTCAAACTCGAATCGGGGGAATGGCTTTGACAGAAATGCTGAAACATCATCCT TAGCATTTAAGCCAACAAAGCAGCTGATGCCCTCTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAG GGCTCTGACCCCTCCTTCCTATAGTACTGCTAAAAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGG AAGTATACGTCGCCAGTCATGAGTGAGCACGGAGACGACCATCGGCAGCTCCTCGCTCATCCAATACAAG GTCCCGGACTCCGTGCAGCTACCTCATCCAACCACTCTGTGGACGAGCAACTGAAGAATACTGACACACA CCTCATTGACCTGGTCACCAATGAGATCATCACCCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGT CTTGACCTGGTGAAGGCGGTTATTAAGGAGGAGGTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTG GGCTGACCGCCTTACCTAGAAGCATCCTTCTCTTCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAG ATGCATAGCTAGTCAGCTAGGGGCCACGTTTTTCAAAATTGCCGGTTCCGGACTCGTTGCAAAGTGGATA GGAGAAGCGGAGAAAATCATCCACGCCTCTTTTCTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTG TTAGTGACATTGACATGCTTCTCTCCTCTCAAGTGAGCGAGGAACACAGTCCAGTCAGTCGGATGAGAAC CGAATTTCTGATGCAGCTGGACACTGTACTGACTTCGGCTGAGGACCAAATCGTAGTCATTTGTGCCACC AGTAAACCAGAAGAAATAGATGAATCTCTTCGGAGGTACTTCATGAAAAGACTTTTAATCCCACTTCCTG ACAGCACAGCGAGGCACCAGATAATAGTACAGCTGCTCACACAGCACAATTACTGTCTCAATGACAAGGA GTTTGCACTGCTTGTCCAGCGCACAGAAGGCTTTTCTGGACTAGACGTGGCTCATTTGTGTCAGGAAGCA GCAGTGGGACCTCTCCATGCTATGCCAGCTACAGACCTTTCAGCCATTATGCCCAGCCAGTTAAGGCCCG TTACATATCAAGACTTTGAAAATGCTTTCTGCAAGATTCAGCCTAGCATATCTCAAAAAGAGCTTGATAT GTATGTTGAATGGAACAAAATGTTTGGTTGCAGTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_021716 |
| Insert Size | 2280 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021716.5, NP_068362.1 |
| RefSeq Size | 9759 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 60344 |
| UniProt ID | Q9ERZ6 |
| Gene Summary | ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, suppressing microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG212262 | Fign (tGFP-tagged) - Mouse fidgetin (Fign), (10ug) |
CNY 6,370.00 |
|
| MR212262 | Fign (Myc-DDK-tagged) - Mouse fidgetin (Fign) |
CNY 5,560.00 |
|
| MR212262L3 | Lenti ORF clone of Fign (Myc-DDK-tagged) - Mouse fidgetin (Fign) |
CNY 7,700.00 |
|
| MR212262L4 | Lenti ORF clone of Fign (mGFP-tagged) - Mouse fidgetin (Fign) |
CNY 7,700.00 |
