Mlh1 (NM_026810) Mouse Untagged Clone

CAT#: MC221356

Mlh1 (untagged) - Mouse mutL homolog 1 (E. coli) (Mlh1), (10ug)



  "NM_026810" in other vectors (4)

CNY 9,790.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110035C23Rik; AI317206; AI325952; AI561766
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221356 representing NM_026810
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTTGTAGCAGGAGTTATTCGGCGTCTGGACGAGACGGTAGTGAACCGCATAGCGGCGGGGGAAG
TCATTCAGCGGCCGGCCAATGCTATCAAAGAGATGATAGAAAACTGTTTAGATGCAAAATCTACAAATAT
TCAAGTGGTTGTTAAGGAAGGTGGCCTGAAGCTAATTCAGATCCAAGACAATGGCACTGGAATCAGGAAG
GAAGATCTGGATATTGTGTGTGAGAGGTTCACTACGAGTAAACTGCAGACTTTTGAGGATTTAGCCAGTA
TTTCTACCTATGGCTTTCGTGGTGAGGCATTGGCAAGCATAAGCCATGTGGCCCATGTCACTATTACAAC
CAAAACAGCTGATGGGAAATGTGCGTACAGAGCAAGTTACTCAGATGGAAAGCTGCAAGCCCCTCCTAAA
CCCTGTGCAGGCAACCAGGGCACCCTGATCACGGTGGAAGACCTTTTTTACAACATAATCACAAGGAGGA
AAGCTTTAAAAAATCCAAGTGAAGAGTACGGAAAAATTTTGGAAGTTGTTGGCAGGTATTCAATACACAA
TTCAGGCATTAGTTTCTCAGTTAAAAAACAAGGTGAGACAGTATCTGATGTCAGAACACTGCCCAATGCC
ACAACCGTGGACAACATTCGCTCCATCTTTGGAAATGCGGTTAGTCGAGAACTGATAGAAGTTGGGTGTG
AGGATAAAACCCTAGCTTTCAAAATGAATGGCTATATATCGAATGCAAACTATTCAGTGAAGAAGTGCAT
TTTCCTACTCTTCATCAACCACCGTCTGGTAGAATCAGCTGCCTTGAGAAAAGCCATTGAAACTGTATAT
GCAGCATACTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTGGAAATCAGCCCTCAGAACGTGG
ACGTCAATGTACACCCCACCAAGCACGAAGTTCACTTTCTGCACGAGGAGAGCATTCTGCAGCGTGTGCA
GCAGCACATTGAGAGCAAGCTGCTGGGCTCCAATTCCTCCAGGATGTATTTCACCCAGACCTTGCTTCCA
GGACTTGCTGGGCCTTCTGGGGAGGCAGCTAGACCCACGACAGGGGTGGCTTCCTCATCCACTAGTGGAA
GTGGCGACAAGGTCTACGCTTACCAGATGGTCCGTACGGACTCCCGGGAGCAGAAGCTTGACGCCTTTCT
GCAGCCTGTAAGCAGCCTTGGGCCCAGCCAGCCCCAGGACCCTGCCCCTGTCCGAGGGGCCAGGACGGAG
GGATCTCCTGAAAGGGCCACGCGGGAGGACGAGGAGATGCTTGCTCTCCCAGCCCCCGCTGAAGCAGCTG
CTGAGAGTGAGAACTTGGAGAGGGAATCACTAATGGAGACTTCAGACGCAGCCCAGAAGGCGGCACCCAC
TTCCAGTCCAGGAAGCTCCAGAAAGAGACATCGGGAGGACTCTGATGTGGAAATGGTGGAAAATGCTTCC
GGGAAGGAAATGACAGCTGCTTGCTACCCCAGGAGGAGGATCATTAACCTCACCAGCGTCTTGAGTCTCC
AGGAAGAGATTAGTGAGCGGTGCCATGAGACTCTCCGGGAGATGCTCCGTAACCATTCCTTTGTGGGCTG
TGTGAATCCTCAGTGGGCCTTGGCACAGCACCAGACCAAGCTCTACCTCCTCAACACTACCAAGCTCAGT
GAAGAGCTGTTCTACCAGATACTCATTTATGATTTTGCCAACTTTGGTGTTCTGAGGTTATCGGAACCAG
CGCCACTCTTCGACCTGGCCATGCTGGCCTTAGACAGTCCTGAAAGTGGCTGGACAGAGGACGACGGCCC
GAAGGAAGGGCTTGCAGAGTACATTGTTGAGTTTCTGAAGAAGAAAGCGGAGATGCTTGCAGACTATTTC
TCTGTGGAGATCGATGAGGAAGGGAACCTGATTGGATTACCTCTTCTGATTGACAGCTATGTGCCACCTT
TGGAGGGACTGCCTATCTTCATTCTTCGACTGGCCACTGAGGTGAATTGGGATGAAGAAAAGGAGTGTTT
TGAAAGTCTCAGTAAAGAATGTGCTATGTTTTACTCCATTCGGAAGCAGTATATACTGGAGGAGTCGACC
CTCTCAGGCCAGCAGAGTGACATGCCTGGCTCCACGTCAAAGCCCTGGAAGTGGACTGTGGAGCACATTA
TCTATAAAGCCTTCCGCTCACACCTCCTACCTCCGAAGCATTTCACAGAAGATGGCAATGTCCTGCAGCT
TGCCAACCTGCCAGATCTATACAAAGTCTTTGAGCGGTGTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_026810
Insert Size 2283 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_026810.2, NP_081086.2
RefSeq Size 2598 bp
RefSeq ORF 2283 bp
Locus ID 17350
UniProt ID Q9JK91
Gene Summary Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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