Pik3r1 (NM_001077495) Mouse Untagged Clone

CAT#: MC221084

Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2, (10ug)



  "NM_001077495" in other vectors (4)

CNY 9,310.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
Pik3r1 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms p50alpha; p55alpha; p85alpha; PI3K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221084 representing NM_001077495
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCAGAGGGCTACCAGTACAGAGCACTGTACGACTACAAGAAGGAGCGAGAGGAAGACATTGACC
TACACCTGGGGGACATACTGACTGTGAATAAAGGCTCCTTAGTGGCACTTGGATTCAGTGATGGCCAGGA
AGCCCGGCCTGAAGATATTGGCTGGTTAAATGGCTACAATGAAACCACTGGGGAGAGGGGAGACTTTCCA
GGAACTTACGTTGAATACATTGGAAGGAAAAGAATTTCACCCCCTACTCCCAAGCCTCGGCCCCCTCGAC
CGCTTCCTGTTGCTCCGGGTTCTTCAAAAACTGAAGCTGACACGGAGCAGCAAGCGTTGCCCCTTCCTGA
CCTGGCCGAGCAGTTTGCCCCTCCTGATGTTGCCCCGCCTCTCCTTATAAAGCTCCTGGAAGCCATTGAG
AAGAAAGGACTGGAATGTTCGACTCTATACAGAACACAAAGCTCCAGCAACCCTGCAGAATTACGACAGC
TTCTTGATTGTGATGCCGCGTCAGTGGACTTGGAGATGATCGACGTACACGTCTTAGCAGATGCTTTCAA
ACGCTATCTCGCCGACTTACCAAATCCTGTCATTCCTGTAGCTGTTTACAATGAGATGATGTCTTTAGCC
CAAGAACTACAGAGCCCTGAAGACTGCATCCAGCTGTTGAAGAAGCTCATTAGATTGCCTAATATACCTC
ATCAGTGTTGGCTTACGCTTCAGTATTTGCTCAAGCATTTTTTCAAGCTCTCTCAAGCCTCCAGCAAAAA
CCTTTTGAATGCAAGAGTCCTCTCTGAGATTTTCAGCCCCGTGCTTTTCAGATTTCCAGCCGCCAGCTCT
GATAATACTGAACACCTCATAAAAGCGATAGAGATTTTAATCTCAACGGAATGGAATGAGAGACAGCCAG
CACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGTAGCCAACAACAGCATGAACAACAATATGTC
CTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAACTCCGAGACACT
GCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAATGCACGGCGATTACACTCTTACACTAAGGA
AAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTAAC
CTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTTAGCTCAGTACAACCCCAAGCTG
GATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGGATCAAGTTGTCAAAGAAGATAATATTGAAG
CTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGATAGATTATA
TGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAAAGAACGGCTATCGAAGCATTTAATGAAACC
ATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAAAC
GCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATAAGCTGAAGTCGCGTATCAGTGA
GATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTACCGAGAGATCGAC
AAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAATACTTGATGTGGC
TGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTGGCTGGGGAATGAAAATACCGAAGATCAATA
CTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCAAC
CGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTCCTTGTCCGGGAGAGCAGTAAGC
AGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGTCAAGCATTGCGTCATTAACAAGACTGCCAC
CGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTACATTATCAACAC
ACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACACTAGCATACCCAGTATATGCACAACAGAGGC
GATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001077495
Insert Size 2175 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001077495.2, NP_001070963.1
RefSeq Size 6928 bp
RefSeq ORF 2175 bp
Locus ID 18708
UniProt ID P26450
Gene Summary Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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