Grin1 (NM_001177657) Mouse Untagged Clone
CAT#: MC220724
Grin1 (untagged) - Mouse glutamate receptor, ionotropic, NMDA1 (zeta 1) (Grin1), transcript variant 3, (10ug)
CNY 9,120.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | GluN1; GluRdelta1; GluRzeta1; M100174; NMD-R1; Nmdar; NMDAR1; NR1; Rgsc174 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC220724 representing NM_001177657
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTCTCCTGCTCCTTCGCCCGCGCTGCCTGCGACC CCAAGATTGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCACGAGCAGATGTTCCGCGAGGCAGTAAA CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGTC ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC CGTCCTGGGGCTGACTACCCGAATGTCCATCTACTCTGACAAGAGCATCCACCTGAGCTTCCTTCGCACC GTACCACCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGCGTCTACAACTGGAACCATATCA TCCTGCTGGTCAGCGATGACCACGAGGGCCGGGCAGCGCAGAAGCGCCTGGAGACGTTGCTGGAGGAGCG TGAGTCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAA GCCCGGGACCTGGAAGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACCGTATACCGCG CAGCCGCGATGCTGAACATGACTGGCTCTGGGTACGTGTGGCTCGTCGGGGAGCGCGAGATCTCTGGGAA TGCCCTGCGCTACGCTCCTGACGGCATCATCGGACTTCAGCTAATCAACGGCAAGAACGAGTCGGCCCAC ATCAGTGACGCTGTGGGCGTGGTGGCACAGGCAGTCCACGAGCTCCTAGAAAAGGAGAACATCACTGATC CACCGCGGGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCACTGTTCAAGAGGGTGCTGATGTC TTCCAAGTATGCAGATGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAAC TATAGTATCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCC CAAATGACAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAGCCTCGAGGATACCAGATGTCCACCAG ACTAAAGATAGTGACAATCCACCAAGAACCCTTCGTGTATGTCAAGCCCACAATGAGTGATGGCACATGC AAAGAGGAGTTCACAGTCAATGGTGACCCTGTCAAGAAGGTGATCTGTACGGGGCCTAATGACACATCCC CAGGAAGCCCACGTCACACAGTGCCCCAGTGCTGTTATGGCTTCTGCGTTGACCTGCTCATCAAGCTGGC ACGGACCATGAATTTTACCTACGAGGTGCACCTTGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTA AACAACAGCAACAAAAAGGAGTGGAACGGAATGATGGGAGAGCTGCTCAGTGGTCAAGCAGACATGATCG TGGCTCCACTGACCATTAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGG CCTGACCATTCTGGTCAAGAAGGAGATCCCTCGGAGCACACTGGACTCATTCATGCAGCCCTTTCAGAGC ACACTGTGGCTGCTGGTGGGGCTGTCAGTTCATGTGGTGGCCGTGATGCTGTACCTGCTGGACCGCTTCA GTCCCTTTGGCCGATTTAAGGTGAACAGCGAGGAGGAGGAGGAGGATGCACTGACCCTGTCCTCTGCCAT GTGGTTTTCCTGGGGCGTCCTGCTCAACTCTGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCTCGT ATCCTAGGCATGGTGTGGGCTGGTTTTGCCATGATCATCGTGGCTTCCTACACTGCCAACCTGGCAGCCT TCCTGGTGCTGGATAGGCCTGAGGAGCGCATCACAGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGA CAAGTTCATCTATGCAACTGTAAAACAGAGCTCTGTGGATATCTACTTCCGGAGGCAGGTGGAGTTGAGC ACCATGTACCGGCACATGGAGAAGCACAATTATGAGAGTGCAGCTGAGGCCATCCAGGCTGTGCGGGACA ACAAGCTCCATGCCTTCATCTGGGACTCAGCTGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGT GACCACGGGTGAGCTGTTCTTCCGCTCCGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAA AATGTGTCCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGACCTGGATAAGACATGGGTTC GGTATCAAGAATGTGACTCCCGCAGCAATGCCCCTGCCACCCTCACTTTTGAGAACATGGCAGGGGTCTT CATGCTGGTGGCTGGAGGCATCGTAGCTGGGATCTTCCTCATTTTCATCGAGATCGCCTACAAGCGACAC AAGGATGCCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGC AGTACCATCCCACTGATATCACGGGCCCGCTCAACCTCTCAGATCCCTCGGTCAGCACCGTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001177657 |
Insert Size | 2658 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001177657.2, NP_001171128.1 |
RefSeq Size | 3859 bp |
RefSeq ORF | 2658 bp |
Locus ID | 14810 |
UniProt ID | P35438 |
Gene Summary | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1532151, PubMed:8060614, PubMed:12008020). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:12008020).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an exon and contains an alternate 3' terminal exon, resulting in an alternate 3' coding region, compared to variant 1. The encoded isoform (3) has distinct C-terminus and is shorter than isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR211057 | Grin1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA1 (zeta 1) (Grin1), transcript variant 3 |
CNY 8,840.00 |
|
MR211057L3 | Lenti ORF clone of Grin1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA1 (zeta 1) (Grin1), transcript variant 3 |
CNY 10,740.00 |
|
MR211057L4 | Lenti ORF clone of Grin1 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, NMDA1 (zeta 1) (Grin1), transcript variant 3 |
CNY 10,740.00 |