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Zc3hav1 (BC029090) Mouse Untagged Clone
CAT＃: MC220698
Zc3hav1 (untagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558), (10ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1200014N16, 2900058M19, ZAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC029090
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGATCCCGAGGTATTCTGTTTCATCACCAAGATCCTGTGCGCTCACGGGGGCCGCATGACCCTGG AGGAACTGCTGGGTGAGATCAGCCTCCCCGAAGCGCAACTCTACGAGCTGCTGAAGGCAGCGGGGCCCGA TCGCTTTGTGCTATTGGAGACTGGAGACCAGGCCGGGATCACTCGGTCGGTGGTGGCTACTACTCGAGCC CGCGTCTGCCGTCGCAAGTACTGCCAGAGACCCTGCGACAGCCTGCACCTTTGCAAGCTTAATCTGCTCG GCCGGTGCCACTATGCACAGTCCCAGCGGAACCTCTGCAAATATTCTCACGATGTTCTCTCGGAACAGAA CTTCCAGGTCCTGAAGAATCATGAGCTCTCCGGGCTTAACCAAGAGGAGCTGGCGGTCCTCCTGGTCCAA AGCGACCCTTTCTTCATGCCTGAGATATGCAAGAGTTACAAAGGAGAGGGCCGCAAACAGATCTGCGGGC AGCCGCAGCCCTGCGAGAGACTCCACATCTGTGAGCACTTCACCCGGGGCAACTGCAGTTACCTCAACTG TCTCAGGTCTCATAACCTGATGGACAGGAAGGTGTTGGCCATCATGAGGGAGCATGGGCTGAGTTCTGAC GTGGTCCAGAACATCCAGGACATCTGTAACAACAAACACACTCGGAGGAACCCCCCTAGCATGAGAGCTC CCCACCCACATCGCAGAGGCGGGGCACACAGGGACAGAAGCAAAAGCAGAGACCGCTTCCATCACAACAG TCTAGAGGTTCTCTCAACGGTCTCACCTCTGGGGTCTGGTCCCCCTAGCCCAGATGTCACCGGCTGTAAG GATCCCCTGGAGGATGTGTCTGCAGATGTCACCCAGAAGTTCAAGTACCTGGGGACTCAGGACCGTGCAC AGCTTTCCTCCGTCTCATCTAAGGCCGCTGGTGTCCGAGGACCCAGTCAAATGAGAGCAAGCCAGGAGTT TTTGGAGGATGGGGATCCAGATGGCTTGTTTTCTAGGAATCGTTCTGATTCGTCCACAAGTCGAACCTCT GCTGCTGGCTTTCCTCTCGTTGCGGCACAAAGAAATGAAGCTGGGGCCATGAAAATGGGCATGCCTTCAG GACACCACGTCGAGGTCAAGGGCAAGAACAAGGACATTGATCGCGTCCCGTTTTTAAATAGTTATATTGA TGGGGTAACAATGGAAGAAGCAACAGTTTCAGGAATTCTAGGTAAAAGGGCCACAGACAACGGTCTGGAA GAAATGATACTATCTAGCAACCATCAGAAGAGTGTGGCTAAGACCCAGGATCCCCAGACCACTGGCAGAA TCACTGACAGTGGCCAAGACACGGCATTCCTGCATAGTAAATATGAAGAAAACCCAGCGTGGCCAGATTC CCTATGCTACATCGTAAATCCTGTATCTCCTAGGATGGATGATCATGGCCTGAAGGAAATCTGTCTGGAT CATCTGTACAGGGGCTGTCAGCAGGTCAACTGCAACAAGAACCACTTCCATCTGCCCTACCGGTGGCAGC TGTTCATATTGCCCACTTGGATGGACTTTCAGGACATGGAGTATATCGAGCGGGCCTATTGTGATCCCCA AATTGAAATCATTGTGATAGAAAAACATCGGATCAATTTCAAGAAAATGACTTGTGATTCCTACCCCATC CGTCGCCTCTCCACTCCTTCATTTGTCGAAAAAACACTTAATTCTGTCTTCACCACCAAGTGGCTTTGGT ATTGGAGGAATGAATTGAATGAATATACTCAGTATGGGCATGAGAGCCCAGGCCATACCAGCTCCGAAAT TAATTCTGCATACCTGGAGTCTTTCTTCCACTCCTGTCCCAGGGGAGTTTTGCAGTTCCACGCTGGTTCA CAGAATTACGAGTTAAGCTTTCAAGGGATGATTCAGACGAATATAGCTTCCAAGACTCAAAGGCATGTTG TGAGAAGGCCAGTTTTTGTTTCTTCGAAGGATGTGGAGCAGAAGAGAAGAGGTCCAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC029090 |
| Insert Size | 2022 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029090, AAH29090 |
| RefSeq Size | 2980 bp |
| RefSeq ORF | 2021 bp |
| Locus ID | 78781 |
| Gene Summary | Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1) and moloney and murine leukemia virus (MoMLV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of DDX58/RIG-I signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209944 | Zc3hav1 (tGFP-tagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558) |
CNY 4,180.00 |
|
| MR209944 | Zc3hav1 (Myc-DDK-tagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558) |
CNY 4,928.00 |
|
| MR209944L3 | Lenti ORF clone of Zc3hav1 (Myc-DDK-tagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558) |
CNY 5,700.00 |
|
| MR209944L4 | Lenti ORF clone of Zc3hav1 (mGFP-tagged) - Mouse zinc finger CCCH type, antiviral 1 (cDNA clone MGC:27750 IMAGE:2651558) |
CNY 5,700.00 |
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