Lcmt2 (NM_177846) Mouse Untagged Clone

CAT#: MC220477

Lcmt2 (untagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2), (10ug)



  "NM_177846" in other vectors (4)

CNY 8,740.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms D330024M17; Tyw4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220477 representing NM_177846
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCGCGAGGCCGCCAGCGCCGGGCGGGAACGGTCCAGAGCACCAACGACAGCAGCTCCCTCAGCA
AACGATCGCTTGCCGCACACGGATACGTGCGCGACCCCTTTGCGGCGCTGCTGGTCCCGGGACCCGTGCG
GCGCACGCCGCTTATCCACCGCGGCTATTACGTGCGCGCGCGCGCCGTGCGTCACTGCGTGCGCGCCTTC
CTCGAACTCACTAGCGCGCTCCCGTCCCGGACCAGAGCCCAGATCCTGTCCTTGGGCTCAGGCTCCGACT
CGCTTTATTTTCGTCTGAAAGCGGCGGGCCTCCTGGCCCGAGCTGCCGTCTGGGAGGTGGACTTCCCGGA
CGTGTCTCGGCTCAAGGCGGAGAGGATCGAGGAGACCCCGGAGCTGCGTGCGCAGACCGGTCCTTTCAAG
ATCGGGGACTCGGCGTCATCTCTGTGCTTCGAGAGCGCGGACTACCGCATCCTGGGCGCGGACCTGCGGG
AGCTCCAGCGATTAGGAGAGGCCCTGGATGGCGCCGGCCTGGACGCCACCTCTCCCACGCTGCTCCTGGC
CGAGGCGGTGCTGACCTACTTGGAGCCCTCCAGTGCCACAGCCCTCATTGCCTGGGCCGCCCAGCGTTTC
CCCGACGCCCTTTTCGTGATCTATGAGCAGATGCAGCCGGGGGATGCCTTTGGGCAGATCATGCTGCAGC
ACTTTCAGCGGTTGCATTCACCCTTGCATGGCCTGGAGCTGTTTCCCGTCGTGAAGGCCCAGCGCCAGCG
CTTCCTTCAAGCTGGCTGGACTGCCTGCAGCGCCCTGGACCTGAACGAGTTCTATCGCCGCCTTCTCTCG
GCAGAAGAGCGCCAGCGGGTTGAGACGCTTGAACCCTTTGATGAATATGAGGAGTGGCATCTGAAGTGTT
CACATTATTTCATCCTGGCAGCATCTAGGGGAGACATTCTGTCAGAAACTCCAGTGTTTGAGCCCTCAGA
GGCTTCTTTTCAGATAGATCCTGCCTCGCCTTCAGGGTTTCTTTCTGCCAGAGTAGTCACTAGTGACCAC
CAGCACTCAAGCCTGAAGCGATATGGCCACGCCTCTGCCCTCTTGAGCCCTGGCGTTATTTTCAGTGCAG
GAGGCTTTGGAGAACAAGAGGGACGACACTGCCGAGTGAGCAGGTTTCACGTGCTCTCCAGATCCTGTGA
CTCGGAATGGGAAGGCTGCCAAATAAGTACCTTGGGGACTGAAGGCCAGTGGGATGGACGCCTTTATCAC
ACCATGACAAGGCTTTCTGATACTCGGGTTCTGGTTCTTGGAGGAAGACTCTCCCCAGTAAGTCCAGCCT
CTGGGGCTCTCCAACTTGATTTATACAAAAGTAAGGATAATTGCTCTGAGGGCCAAAATGTAACAGTAAC
GAAGGCTGCCTTGGAAGAAGGTTCAGTGTTGTCATGTTGGCGGCATTCAACAACGGAAGTATACTATCAG
AATCAAAGATACTTATTTGTGTATGGTGGCCGAAGTGTGGCTGAACCTGTACTAAGTGACTGTCGTTTTC
TACATGTAGAGACAATGGCTTGGGTCAGAATCCCAGTACAAGGCGCATCACCCGAAGGTCGGCATTCCCA
TAGCGCCTGCAGTTGGCAAGGGGGAGCACTTATCGCTGGAGGTCTTGGGGCTTCTGAGGAACTGTTGAGC
TCTGTACTCTTTCTCAAACCAGTGTCCTCTGGATTCCTCTGGGAATCAATAGATATCCAGCCCTCCATTA
CCCCAAGGTACTCTCACACGGCTCATGTGTTTAATGGAAAGCTGCTTCTGGTTGGAGGGGTCTGGATTCA
TTCCTCCTCAGTTCCTGGAGTGACTGTTATCTGTTTGACTACAGGGTTGAGCTCTGAATATCAGATTGAC
ACAGCGTCTGTGCCATGGCCATTAATGCTGCACAACCACAGCAGCGCCCTCCTTCCTGAAGAGCAGCAGC
TCCTGCTTATTGGAGGTGGAGGGAACTGCTTTTCCTTTGGTACTTATTTCAACCCCCACACAGTGGCATT
AGACCTTTCTTCCTTACGTTCAGGGCAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_177846
Insert Size 2061 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_177846.3, NP_808514.2
RefSeq Size 3407 bp
RefSeq ORF 2061 bp
Locus ID 329504
UniProt ID Q8BYR1
Gene Summary Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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