Atf6 (NM_001081304) Mouse Untagged Clone

CAT#: MC220203

Atf6 (untagged) - Mouse activating transcription factor 6 (Atf6), (10ug)



  "NM_001081304" in other vectors (4)

CNY 8,460.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-Atf6 Antibody
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9130025P16Rik; 9630036G24; AA789574; Atf6alpha; ESTM49
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220203 representing NM_001081304
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCGCCTTTTAGTCCGGTTCTTCCTCATGGACCAGATGAAGACTGGGAGTCGACGTTGTTTGCTG
AACTTGGCTATTTCACAGACACTGATGATGTGCACTTTGATGCAGCACATGAGGCTTATGAAAATAATTT
TGATCATCTTAATTTTGATTTGGATTTGATGCCTTGGGAGTCAGACCTATGGAGCCCCGGCAGCCACTTC
TGCTCAGACATGAAGGCAGAGCCCCAGCCTCTTTCTCCGGCTTCCTCCAGTTGCTCCATCTCCTCTCCTC
GGTCCACAGACTCGTGTTCTTCAACTCAGCACGTTCCTGAGGAGTTGGATTTGTTGTCTAGTTCTCAGTC
CCCCCTTTCCTTATATGGCGACAGCTGTAATAGCCCCTCCTCTGTAGAGCCACTGAAGGAAGAGAAGCCT
GTCACTGGTCCTGGAAACAAAACAGAACATGGACTGACTCCAAAGAAAAAAATTCAGATGAGTTCAAAAC
CTTCAGTTCAGCCCAAGCCTTTATTACTTCCAGCAGCGCCCAAGACTCAAACCAATGCCAGTGTCCCAGC
AAAAGCCATCATCATTCAGACACTACCAGCCCTTATGCCACTGGCAAAGCAGCAGTCGATTATCAGCATA
CAGCCTGCGCCCACCAAAGGCCAGACTGTTTTGCTCTCTCAGCCGACTGTGGTTCAACTTCAGAGCCCTG
CGGTTCTGTCGTCTGCTCAGCCGGTTCTTGCAGTCACTGGGGGAGCCGCACAGCTACCTAACCATGTGGT
GAATGTGCTGCCAGCCCCTGTGGTGAGCAGCCCGGTGAATGGAAAACTTTCCGTGACTAAACCTGTTCTA
CAAAGTGCCACCAGAAGTATGGGTTCGGATATCGCTGTGCTGAGGAGACAGCAGCGGATGATAAAGAACC
GAGAGTCTGCTTGTCAGTCGCGCAAGAAGAAGAAAGAGTATATGCTAGGACTGGAGGCCAGGCTCAAGGC
TGCCCTCTCAGAGAATGAGCAGCTGAAGAAGGAGAATGGCTCCCTGAAGCGACAGCTGGACGAGGTGGTG
TCAGAGAACCAGAGGCTCAAAGTCCCAAGTCCAAAGCGAAGAGCTGTCTGTGTGATGATAGTATTAGCAT
TTATAATGCTGAACTATGGGCCCATGAGCATGCTGGAGCAAGAATCCCGAAGAGTGAAACCTAGTGTGAG
CCCTGCCAATCAGAGGAGGCATCTCTTGGAATTTTCAGCAAAAGAAGTTAAAGACACATCAGATGGTGAC
AACCAGAAAGACAGTTACAGCTATGATCACTCTGTGTCCAATGACAAAGCTTTAATGGTGCTAAGTGAAG
AGCCATTGCTTTATATGCCTCCACCTCCATGTCAACCCCTGATTAACACAACAGAGTCTCTCAGGTTGAA
CCATGAACTTCGAGGCTGGGTTCATAGACATGAAGTGGAAAGGACCAAATCTAGAAGAATGACAAATAGC
CAACAGAAAGCCCGCATTCTCCAGGGTGCTCTGGAACAGGGCTCTAATTCTCAGCTGATGGCTGTCCAGT
ACACAGAAACCACTAGCATCAGTAGGAATTCTGGGAGTGAGCTGCAAGTGTATTACGCCTCCCCTGGAAG
TTACCAAGGCTTCTTTGACGCCATCCGCAGGAGGGGAGATACGTTTTACGTTGTCTCATTTCGAAGGGAT
CATCTGCTATTACCAGCTACCACCCACAACAAGACCACAAGACCAAAAATGTCAATTGTATTACCAGCAA
TAAACATAAATGATAATGTGATCAATGGGCAGGACTATGAAGTAATGATGCAGATTGACTGTCAGGTGAT
GGACACCAGGATCCTCCACATCAAAAGCTCCTCGGTTCCCCCTTATCTCCGGGATCATCAGCGGAACCAA
ACCAGCACCTTCTTTGGTTCCCCTCCAACAACCACAGAGACGACCCATGTGGTCAGCACCATCCCTGAGT
CGTTGCAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081304
Insert Size 1971 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081304.1, NP_001074773.1
RefSeq Size 7463 bp
RefSeq ORF 1971 bp
Locus ID 226641
UniProt ID F6VAN0
Gene Summary Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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