Def6 (NM_027185) Mouse Untagged Clone

CAT#: MC219909

Def6 (untagged) - Mouse differentially expressed in FDCP 6 (Def6), (10ug)



  "NM_027185" in other vectors (6)

CNY 8,080.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410003F05Rik; 6430538D02Rik; AV094905; Ibp; Slat; Slat2; Slat6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219909 representing NM_027185
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGCGCAAGGAGCTGCTCAAGTCTATCTGGTACGCCTTCACTGCACTGGACGTGGAGAAGAGCG
GCAAGGTCTCCAAGTCCCAACTCAAGGTGCTGTCCCACAACCTGTACACTGTCCTGAACATCCCCCATGA
CCCCGTGGCCCTGGAGGAGCACTTCCGGGATGACGATGATGGCCCGGTGTCCAGTCAAGGTTACATGCCC
TACCTCAACAAGTACATCCTAGACAAGGTGGAGGAGGGAGCTTTCGTTAAGGAGCACTTCGATGAGTTGT
GCTGGACCCTGACTGCCAAGAAGAACTACAGGGCAGATGGCATCGGGAGCAGCCCACTCTCTAATCAGGA
TGCTTTCCGTCTATGGTGCCTCTTCAACTTCCTGTCTGAGGACAAGTACCCGCTGATCATGGTTCCCGAT
GAGGTAGAGTATCTGCTGAAGAAGCTCCTTGGCAGCCTGAGTTTGGAGATGGGCCTGGGCAAGCTGGAGG
AGCTGCTGGCCCAGGACGCCCAGTCAGCCCAGACCGCCGTGGGGCTCAGCGTCTGGCAGTTTCTGGAACT
CTTCAACTCAGGCCGCTGCCTTCGGGGTGTGGGGCGCGACTCCCTCAGCATGGCCATCCAAGAAGTCTAC
CAGGAGCTCATCCAAGACGTCCTGAAGCAGGGCTATCTGTGGAAGCGAGGGCACCTGCGGAGGAACTGGG
CCGAGCGCTGGTTCCAGCTGCAACCCAGCAGCCTCTGCTACTTTGGGAGTGAGGAATGCAAGGAGAAACG
AGGCACCATTCCCCTGGATGCTCACTGCTGTGTGGAGGTGCTTCCGGACCGCGAAGGAAAGCGCTGCATG
TTTTGTGTGAAGACTGCCAGCCGCACCTATGAGATGAGCGCCTCAGACACCCGCCAGCGCCAGGAGTGGA
CGGCCGCCATCCAGACTGCGATCCGGCTGCAGGCGGAGGGGAAGACGTCGCTGCACAAGGACCTGAAACA
GAAGCGGCGGGAGCAGCGGGAACAGCGCGAGCGACGCCGGGCAGCCAAGGAGGAGGAGCTGCTGCGACTG
CAGCAGCTGCAGGAGGAGAAGGAGAGGAAGCTGCAAGAACTGGAGCTGCTGCAGGAGGCTCAGCGGCAGG
CCGAGCGGCTGCTGCAGGAAGAGGAGGAGCGCCGCCGTAGCCAGCACAAGGAGCTGCAGCAGGCTCTGGA
GGGCCAGCTGCGGGAGGCGGAGCAGGCCCGGGCCTCTATGCAGGCTGAGATGGAGCTGAAGAAGGAAGAG
GCGGCCCGGCAACGGCAGCGCATCGCTGAACTGGAGGAGATGCAGGAGCGGCTGCAGGAAGCTCTGCAAC
TAGAGGTGAAAGCTAGGCGGGATGAGGAGGCCGTGCGCCTCGCCCAGACCAGGCTGCTGGAGGAGGAGGA
GGAGAAGCTGAAGCAGCTGATGCATCTGAAGGAGGAGCAAGAGCGCTACATCGAGCGAGCGCAGCAGGAG
AAGCAGGAGCTCCAGCAGGAGATGGCGCTGCAGAGCCGTTCCCTGCAGCACGCCCAGCAGCAGCTGGAGG
AAGTGCGGCAGAACCGGCAGAGGGCAGACGAGGACGTGGAGGCTGCCCAGAGGAAGTTGCGCCAGGCCAG
CACCAACGTGAAACACTGGAATGTCCAGATGAACAGGCTCATGCATCCGATCGAGCCAGGAGATAAGCGA
CCCACCACCAGCAGCTCCTTCACGGGCTTCCAGCCCCCTCCGCTTGCCCGCCGGGACTCCTCTCTAAAGC
GCCTGACCCGCTGGGGTTCCCAAGGTAACAGAACCCTCTCAGTCAATAGCAGCGAACAGAAGTCCCTCAA
TGGTGGAGATGAGACTCCCATCCTAGCTTTGGCCTCTCAGGAAGAAAAACTGGATCCAGCACCAGGGAAT
TAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027185
Insert Size 1893 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027185.3, NP_081461.2
RefSeq Size 2294 bp
RefSeq ORF 1893 bp
Locus ID 23853
UniProt ID Q8C2K1
Gene Summary Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling. Required for optimal T-cell effector function, lymphocyte homeostasis and the prevention of systemic autoimmunity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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