Ache (NM_009599) Mouse Untagged Clone

CAT#: MC219690

Ache (untagged) - Mouse acetylcholinesterase (Ache), (10ug)



  "NM_009599" in other vectors (4)

CNY 6,864.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms mE1a; mE1b; mE1c; mE1c-long; mE1d; mE1d'; mE1e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219690 representing NM_009599
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCCTCCCTGGTATCCCCTGCATACACCTTCCCTGGCTTTTCCACTCCTCTTCCTCCTCCTCTCCC
TCCTGGGAGGAGGGGCAAGGGCTGAGGGCCGGGAAGACCCGCAGCTGCTGGTGAGGGTTCGAGGGGGCCA
GCTGAGGGGCATCCGCCTGAAGGCCCCTGGAGGCCCAGTCTCAGCTTTTCTGGGCATCCCCTTTGCAGAG
CCACCTGTGGGCTCACGTAGATTTATGCCACCAGAGCCCAAGCGGCCCTGGTCAGGAGTGTTGGATGCTA
CCACCTTCCAAAATGTCTGCTACCAGTACGTGGACACCCTGTACCCTGGGTTTGAGGGTACTGAGATGTG
GAACCCCAACCGAGAGTTGAGTGAAGACTGCCTGTATCTTAATGTGTGGACACCATACCCCAGACCTGCT
TCTCCCACACCTGTCCTCATCTGGATCTATGGGGGTGGTTTCTACAGCGGAGCGGCCTCCTTGGATGTGT
ATGACGGCCGTTTCCTGGCCCAGGTTGAGGGAGCTGTGTTGGTATCTATGAACTACCGAGTGGGAACCTT
TGGCTTCTTGGCCCTACCAGGAAGCAGAGAAGCCCCTGGCAATGTAGGTCTGCTGGATCAACGGCTTGCC
TTGCAATGGGTGCAAGAAAATATTGCAGCCTTTGGGGGCGACCCGATGTCAGTGACTCTGTTTGGGGAGA
GTGCGGGTGCAGCCTCCGTGGGCATGCACATACTGTCCCTGCCCAGCAGGAGCCTCTTCCACAGGGCTGT
CCTCCAGAGTGGCACACCCAATGGGCCCTGGGCCACTGTGAGTGCTGGAGAGGCCAGGCGCAGGGCCACA
CTGCTGGCCCGCCTTGTGGGCTGTCCCCCAGGTGGCGCTGGTGGCAATGACACCGAGCTGATAGCCTGCT
TGAGGACAAGGCCCGCTCAGGACCTGGTGGACCACGAGTGGCACGTCCTGCCTCAAGAAAGTATCTTCCG
ATTTTCCTTCGTGCCTGTGGTAGACGGGGACTTCCTCAGTGACACACCGGAGGCTCTCATCAATACTGGA
GATTTTCAAGACCTGCAGGTGCTGGTGGGTGTGGTGAAGGACGAGGGCTCCTACTTTCTGGTTTACGGGG
TCCCAGGCTTCAGCAAAGACAATGAATCTCTCATCAGCCGGGCCCAGTTCCTGGCTGGGGTGCGGATCGG
TGTACCCCAAGCAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATTACACAGACTGGCTGCACCCTGAG
GACCCTACTCACCTGAGAGATGCCATGAGTGCAGTGGTAGGCGACCACAACGTTGTGTGCCCTGTGGCCC
AGCTGGCTGGGCGACTGGCTGCCCAAGGGGCCCGGGTCTATGCCTACATCTTTGAACACCGTGCCTCCAC
ACTGACTTGGCCCCTCTGGATGGGGGTGCCCCATGGCTATGAAATCGAGTTCATCTTTGGGCTCCCCCTG
GATCCCTCGCTGAACTACACCACGGAGGAGAGGATCTTTGCTCAGCGACTTATGAAATACTGGACCAATT
TTGCCCGCACAGGGGACCCCAATGACCCTCGAGACTCCAAATCTCCACAGTGGCCACCGTACACCACTGC
CGCGCAGCAATATGTGAGCCTGAACCTGAAGCCCTTAGAGGTGCGGCGGGGACTGCGCGCCCAGACCTGC
GCCTTCTGGAATCGCTTTCTCCCCAAATTGCTCAGCGCCACCGATACTCTGGACGAGGCGGAGCGCCAGT
GGAAGGCCGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTATAGCAA
GCAGGAGCGCTGCTCAGACCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_009599
Insert Size 1845 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009599.3, NP_033729.1
RefSeq Size 2224 bp
RefSeq ORF 1845 bp
Locus ID 11423
UniProt ID P21836
Gene Summary Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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